Effect of n-3 fatty acids on the key enzymes involved in cholesterol and triglyceride turnover in rat liver

Ayman Al-Shurbaji, Carin Larsson-Backström, Lars Berglund, Gösta Eggertsen, Ingemar Bjökhem

Research output: Contribution to journalArticle

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Abstract

The effect of long-chain n-3 fatty acids on hepatic key enzymes of cholesterol metabolism and triglyceride biosynthesis was investigated in two rat models. In the first model, rats were intravenously infused for two weeks with a fat emulsion containing 20% of triglycerides in which either n-6 or n-3 fatty acids predominated. The treatment with n-3 fatty acids led to a reduction primarily of serum cholesterol (45%), but also of serum triglycerides (18%). HMG-CoA reductase activity and cholesterol 7α-hydroxylase activity were reduced by 45% and 36%, respectively. There were no significant effects on diacylglycerol acyltransferase (DGAT) or phosphatidate phosphohydrolase (PAP) activities. In the second model, rats were fed a diet enriched with sucrose, coconut oil and either sunflower oil (n-6 fatty acids) or fish oil (long-chain n-3 fatty acid ethyl esters). The treatment with n-3 fatty acids decreased serum triglycerides (41%) and, to a lesser extent, serum cholesterol (17%). Neither glycerol 3-phosphate acyltransferase (GPAT) or DGAT were affected by n-3 fatty acids. In contrast, PAP activity was reduced by 26%. HMG-CoA reductase was not significantly affected, whereas cholesterol 7α-hydroxylase activity was reduced by 36%. The results indicate that part of the TG-lowering effect of long-chain n-3 fatty acids may be mediated by inhibition of the soluble phosphatidate phosphohydrolase. The effect on serum cholesterol may be partly due to inhibition of HMG-CoA reductase.

Original languageEnglish (US)
Pages (from-to)385-389
Number of pages5
JournalLipids
Volume26
Issue number5
DOIs
StatePublished - May 1991
Externally publishedYes

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Omega-3 Fatty Acids
omega-3 fatty acids
Liver
Rats
Triglycerides
triacylglycerols
Cholesterol
cholesterol
phosphatidate phosphatase
Phosphatidate Phosphatase
liver
Hydroxymethylglutaryl CoA Reductases
rats
Enzymes
enzymes
long chain fatty acids
Diacylglycerol O-Acyltransferase
Cholesterol 7-alpha-Hydroxylase
diacylglycerol acyltransferase
Serum

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Organic Chemistry
  • Cell Biology

Cite this

Effect of n-3 fatty acids on the key enzymes involved in cholesterol and triglyceride turnover in rat liver. / Al-Shurbaji, Ayman; Larsson-Backström, Carin; Berglund, Lars; Eggertsen, Gösta; Bjökhem, Ingemar.

In: Lipids, Vol. 26, No. 5, 05.1991, p. 385-389.

Research output: Contribution to journalArticle

Al-Shurbaji, A, Larsson-Backström, C, Berglund, L, Eggertsen, G & Bjökhem, I 1991, 'Effect of n-3 fatty acids on the key enzymes involved in cholesterol and triglyceride turnover in rat liver', Lipids, vol. 26, no. 5, pp. 385-389. https://doi.org/10.1007/BF02537204
Al-Shurbaji, Ayman ; Larsson-Backström, Carin ; Berglund, Lars ; Eggertsen, Gösta ; Bjökhem, Ingemar. / Effect of n-3 fatty acids on the key enzymes involved in cholesterol and triglyceride turnover in rat liver. In: Lipids. 1991 ; Vol. 26, No. 5. pp. 385-389.
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