Effect of milk—borne egf on igf-i mrna production in the small intestine of suckling rats

B. Dvorak, Chun Hwal, C. S. Williams, D. L. McWi11iam, J. A. Dominguez, Anthony F Philipps, O. Koldovsky, R. S. McCuskey

Research output: Contribution to journalArticle

Abstract

Aim: Epidermal growth factor (EGF) is known to be present inmilk, but its physiological function in neonatal digestivetract remains unclear. Insulin-like growth factors (IGFs) arepostulated to be important regulators of somatic growth during the perinatal period. The aim of this study was to evaluate the effect of milk-borne EGF on the synthesis of IGF-I mRNA in the small intestine and liver of the suckling rats. Method:Eight-day old suckling rats underwent intragastric cannulation were machine-fed either a cow milk-based growth factor free ratmilk substrate (RMS) or RMS supplemented with 500ng/ml of EGF(RMS+EGF) for 4 days. Mother-fed litter mates were served asa control. After 4 days of machine feeding, rats were sacrificed and the small intestine (duodenum, jejunum and ileum) and liver were collected. Then, rat IGF-I mRNA levels were determined by using RT-competitive PCR. Results: There were no significant differences in the body weight gain of three experimental groups of rats. In the duodenum, IGF-I mRNA levels weresignificantly lower in RMS+EGF group compared to those in RMSgroup. In the jejunum and ileum, there were no significantdiferrences in IGF-I mRNA levels between experimental groups.ln the liver, IGF-I mRNA levels were lower in RMS+EGF group compared to those in RMS and mother-fed control groups.Conclusion: We have shown that EGF administrated enterally decreased IGF-I mRNA level in the duodenum and liver. These results suggests that EGF in the milk administered enterally affects endogenous production of IGF-I in the small intestine and liver in developing rats.

Original languageEnglish (US)
Pages (from-to)A493-A502
JournalJapanese Journal of Gastroenterology
Volume96
DOIs
StatePublished - 1999
Externally publishedYes

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Epidermal Growth Factor
Small Intestine
Insulin-Like Growth Factor I
Messenger RNA
Duodenum
Liver
Jejunum
Ileum
Milk
Somatomedins
Catheterization
Transforming Growth Factor beta
Weight Gain
Body Weight
Polymerase Chain Reaction
Control Groups
Growth

ASJC Scopus subject areas

  • Gastroenterology

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Effect of milk—borne egf on igf-i mrna production in the small intestine of suckling rats. / Dvorak, B.; Hwal, Chun; Williams, C. S.; McWi11iam, D. L.; Dominguez, J. A.; Philipps, Anthony F; Koldovsky, O.; McCuskey, R. S.

In: Japanese Journal of Gastroenterology, Vol. 96, 1999, p. A493-A502.

Research output: Contribution to journalArticle

Dvorak, B. ; Hwal, Chun ; Williams, C. S. ; McWi11iam, D. L. ; Dominguez, J. A. ; Philipps, Anthony F ; Koldovsky, O. ; McCuskey, R. S. / Effect of milk—borne egf on igf-i mrna production in the small intestine of suckling rats. In: Japanese Journal of Gastroenterology. 1999 ; Vol. 96. pp. A493-A502.
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abstract = "Aim: Epidermal growth factor (EGF) is known to be present inmilk, but its physiological function in neonatal digestivetract remains unclear. Insulin-like growth factors (IGFs) arepostulated to be important regulators of somatic growth during the perinatal period. The aim of this study was to evaluate the effect of milk-borne EGF on the synthesis of IGF-I mRNA in the small intestine and liver of the suckling rats. Method:Eight-day old suckling rats underwent intragastric cannulation were machine-fed either a cow milk-based growth factor free ratmilk substrate (RMS) or RMS supplemented with 500ng/ml of EGF(RMS+EGF) for 4 days. Mother-fed litter mates were served asa control. After 4 days of machine feeding, rats were sacrificed and the small intestine (duodenum, jejunum and ileum) and liver were collected. Then, rat IGF-I mRNA levels were determined by using RT-competitive PCR. Results: There were no significant differences in the body weight gain of three experimental groups of rats. In the duodenum, IGF-I mRNA levels weresignificantly lower in RMS+EGF group compared to those in RMSgroup. In the jejunum and ileum, there were no significantdiferrences in IGF-I mRNA levels between experimental groups.ln the liver, IGF-I mRNA levels were lower in RMS+EGF group compared to those in RMS and mother-fed control groups.Conclusion: We have shown that EGF administrated enterally decreased IGF-I mRNA level in the duodenum and liver. These results suggests that EGF in the milk administered enterally affects endogenous production of IGF-I in the small intestine and liver in developing rats.",
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T1 - Effect of milk—borne egf on igf-i mrna production in the small intestine of suckling rats

AU - Dvorak, B.

AU - Hwal, Chun

AU - Williams, C. S.

AU - McWi11iam, D. L.

AU - Dominguez, J. A.

AU - Philipps, Anthony F

AU - Koldovsky, O.

AU - McCuskey, R. S.

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Y1 - 1999

N2 - Aim: Epidermal growth factor (EGF) is known to be present inmilk, but its physiological function in neonatal digestivetract remains unclear. Insulin-like growth factors (IGFs) arepostulated to be important regulators of somatic growth during the perinatal period. The aim of this study was to evaluate the effect of milk-borne EGF on the synthesis of IGF-I mRNA in the small intestine and liver of the suckling rats. Method:Eight-day old suckling rats underwent intragastric cannulation were machine-fed either a cow milk-based growth factor free ratmilk substrate (RMS) or RMS supplemented with 500ng/ml of EGF(RMS+EGF) for 4 days. Mother-fed litter mates were served asa control. After 4 days of machine feeding, rats were sacrificed and the small intestine (duodenum, jejunum and ileum) and liver were collected. Then, rat IGF-I mRNA levels were determined by using RT-competitive PCR. Results: There were no significant differences in the body weight gain of three experimental groups of rats. In the duodenum, IGF-I mRNA levels weresignificantly lower in RMS+EGF group compared to those in RMSgroup. In the jejunum and ileum, there were no significantdiferrences in IGF-I mRNA levels between experimental groups.ln the liver, IGF-I mRNA levels were lower in RMS+EGF group compared to those in RMS and mother-fed control groups.Conclusion: We have shown that EGF administrated enterally decreased IGF-I mRNA level in the duodenum and liver. These results suggests that EGF in the milk administered enterally affects endogenous production of IGF-I in the small intestine and liver in developing rats.

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