Effect of milk—borne egf on igf-i mrna production in the small intestine of suckling rats

B. Dvorak, Chun Hwal, C. S. Williams, D. L. McWi11iam, J. A. Dominguez, Anthony F Philipps, O. Koldovsky, R. S. McCuskey

Research output: Contribution to journalArticle

Abstract

Aim: Epidermal growth factor (EGF) is known to be present inmilk, but its physiological function in neonatal digestivetract remains unclear. Insulin-like growth factors (IGFs) arepostulated to be important regulators of somatic growth during the perinatal period. The aim of this study was to evaluate the effect of milk-borne EGF on the synthesis of IGF-I mRNA in the small intestine and liver of the suckling rats. Method:Eight-day old suckling rats underwent intragastric cannulation were machine-fed either a cow milk-based growth factor free ratmilk substrate (RMS) or RMS supplemented with 500ng/ml of EGF(RMS+EGF) for 4 days. Mother-fed litter mates were served asa control. After 4 days of machine feeding, rats were sacrificed and the small intestine (duodenum, jejunum and ileum) and liver were collected. Then, rat IGF-I mRNA levels were determined by using RT-competitive PCR. Results: There were no significant differences in the body weight gain of three experimental groups of rats. In the duodenum, IGF-I mRNA levels weresignificantly lower in RMS+EGF group compared to those in RMSgroup. In the jejunum and ileum, there were no significantdiferrences in IGF-I mRNA levels between experimental groups.ln the liver, IGF-I mRNA levels were lower in RMS+EGF group compared to those in RMS and mother-fed control groups.Conclusion: We have shown that EGF administrated enterally decreased IGF-I mRNA level in the duodenum and liver. These results suggests that EGF in the milk administered enterally affects endogenous production of IGF-I in the small intestine and liver in developing rats.

Original languageEnglish (US)
Pages (from-to)A493-A502
JournalJapanese Journal of Gastroenterology
Volume96
DOIs
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Gastroenterology

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    Dvorak, B., Hwal, C., Williams, C. S., McWi11iam, D. L., Dominguez, J. A., Philipps, A. F., Koldovsky, O., & McCuskey, R. S. (1999). Effect of milk—borne egf on igf-i mrna production in the small intestine of suckling rats. Japanese Journal of Gastroenterology, 96, A493-A502. https://doi.org/10.11405/nisshoshi1964.96.41taikai_A493