Effect of microcystin-LR on human placental villous trophoblast differentiation in vitro

Gordon C Douglas, Twanda L. Thirkill, Priyadarsini Kumar, Minerva Loi, Elizabeth D. Hilborn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Microcystin-LR is a cyanobacterial toxin found in surface and recreational waters that inhibits protein phosphatases and may disrupt the cytoskeleton. Microcystins induce apoptosis in hepatocytes at ≤2.0 μM. Nothing is known about the effects of microcystins on human placental trophoblast differentiation and function. The differentiation of villous trophoblasts to form syncytiotrophoblast occurs throughout pregnancy and is essential for normal placental and fetal development. To investigate the effects of microcystin, villous cytotrophoblasts were isolated from term placentas using an established method and exposed to microcystin-LR. Microcystin-LR below the cytotoxic dose of 25 μM did not cause cell rounding or detachment, had no effect on apoptosis, and no effect on the morphological differentiation of mononucleated cytotrophoblasts to multinucleated syncytiotrophoblast. However, secretion of human chorionic gonadotropin (hCG) increased in a microcystin-LR dose-dependent manner. When incubated with l-buthionine sulphoximine (BSO) to deplete glutathione levels, trophoblast morphological differentiation proceeded normally in the presence of microcystin-LR. Microcystin-LR did not disrupt the trophoblast microtubule cytoskeleton, which is known to play a role in trophoblast differentiation. Immunofluorescence studies showed that trophoblasts express organic anion transport protein 1B3 (OATP1B3), a known microcystin transport protein. In comparison to hepatocytes, trophoblasts appear to be more resistant to the toxic effects of microcystin-LR. The physiological implications of increased hCG secretion in response to microcystin-LR exposure remain to be determined.

Original languageEnglish (US)
Pages (from-to)427-439
Number of pages13
JournalEnvironmental Toxicology
Volume31
Issue number4
DOIs
StatePublished - Apr 1 2016

Fingerprint

Trophoblasts
apoptosis
secretion
Microcystins
protein
Chorionic Gonadotropin
pregnancy
phosphatase
toxin
Cytoskeleton
Organic Anion Transporters
Apoptosis
anion
Buthionine Sulfoximine
Hepatocytes
In Vitro Techniques
cyanoginosin LR
effect
Poisons
Phosphoprotein Phosphatases

Keywords

  • Human
  • Human chorionic gonadotropin
  • in vitro
  • Microcystin
  • Placenta

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

Effect of microcystin-LR on human placental villous trophoblast differentiation in vitro. / Douglas, Gordon C; Thirkill, Twanda L.; Kumar, Priyadarsini; Loi, Minerva; Hilborn, Elizabeth D.

In: Environmental Toxicology, Vol. 31, No. 4, 01.04.2016, p. 427-439.

Research output: Contribution to journalArticle

Douglas, Gordon C ; Thirkill, Twanda L. ; Kumar, Priyadarsini ; Loi, Minerva ; Hilborn, Elizabeth D. / Effect of microcystin-LR on human placental villous trophoblast differentiation in vitro. In: Environmental Toxicology. 2016 ; Vol. 31, No. 4. pp. 427-439.
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