Effect of long-term exposure to copper on survival and development of two successive generations of Culex pipiens (Diptera, Culicidae)

Nermeen R. Amer, Sharon P. Lawler, Nawal M. Zohdy, Aly Younes, Wael M. ElSayed, Richard E. Connon

Research output: Contribution to journalArticlepeer-review

Abstract

Aquatic invertebrates can be exposed to copper from various sources, including agricultural applications. For example, concentrations up to 1000 µg L−1 are found within rice fields, where copper-containing formulations are used as fungicides and algaecides. We conducted toxicity tests to study lethal and sublethal effects of copper sulfate pentahydrate on all immature stages across two generations of Culex pipiens mosquitoes as our model organism. Mortality was dose-dependent at concentrations of 500 µg L−1 and above in the first generation, and 125 µg L−1 and above in the second generation. The median lethal concentrations (LC50) of copper sulfate pentahydrate for larval Cx. pipiens were 476 ± 30.60 µg L−1 and 348.67 ± 23.20 µg L−1 for the first and second generations, respectively. Generation one pupation decreased from 96% in controls to 48% at 500 µg L−1, while the second-generation pupation decreased from 96% in controls to 17.5% at 500 µg L−1. Mortality during the pupal stage varied from 2 to 10% at 500 µg L−1 of first and second generations, respectively. Higher levels also delayed development to adulthood in both generations. The duration of the immature period was 14.8 days in controls in both generations, but when exposed at 500 µg L−1 it increased to 18.8 days in the first generation and to 20.5 days in the second generation. The chronic, multi-generation exposures in this study showed greater toxicity than reported for shorter exposures of Cx. pipiens, and confamilial taxa like Culex hortensis and Anopheles hispaniola.

Original languageEnglish (US)
Pages (from-to)351-360
Number of pages10
JournalEcotoxicology
Volume30
Issue number2
DOIs
StatePublished - Mar 2021
Externally publishedYes

Keywords

  • Aquatic insects
  • Chronic effects
  • Copper sulfate
  • Cross-generation effects
  • Mosquito

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

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