Abstract
The aim of the study was to investigate the effects of long-term exposure (45 days) to growth promoters: clenbuterol (CB, 1mgkg-1bw) and/or dexamethasone (DEX, 0.1mgkg-1bw), in ovarian morphology, and the possibility of recovery after the withdrawal of drug treatment. Animals were sacrificed at different days of withdrawal (W0, W5, W10, W15 and W20). Ovaries of CB treatment showed typical features of long-term administration of β-agonists at W0 with antral and atretic follicles, and the absence of corpora lutea, indicating disrupted cyclicity of rats. However, at W20 ovaries showed cyclic features with corpora lutea, antral follicles, and even recently ovulated follicles. Ovaries of CB+DEX and DEX treatments showed features of disrupted cyclicity at W0 also with cystic follicles, and fewer healthy antral follicles than those of treatment I, these same features also being observed at W20. Immunohistochemistry (IHC) of ovaries denoted that neither positive staining nor localization of steroidogenic enzymes were affected by treatments. We can conclude that treatments can disrupt the reproductive function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-240 |
| Number of pages | 8 |
| Journal | Analytica Chimica Acta |
| Volume | 483 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Apr 25 2003 |
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Keywords
- Clenbuterol
- Dexamethasone
- Growth promoters
- Immunohistochemistry
- Ovarian morphology
- Rats
- Steroidogenic enzymes
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Effect of long-term exposure to combinations of growth promoters in Long Evans rats : Part 2: Ovarian morphology. / Illera, J. C.; Silván, G.; Martínez-Mateos, M. M.; Conley, Alan J; Corbin, J.; Blass, A.; Lorenzo, P. L.; Illera, M.
In: Analytica Chimica Acta, Vol. 483, No. 1-2, 25.04.2003, p. 233-240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of long-term exposure to combinations of growth promoters in Long Evans rats
T2 - Part 2: Ovarian morphology
AU - Illera, J. C.
AU - Silván, G.
AU - Martínez-Mateos, M. M.
AU - Conley, Alan J
AU - Corbin, J.
AU - Blass, A.
AU - Lorenzo, P. L.
AU - Illera, M.
PY - 2003/4/25
Y1 - 2003/4/25
N2 - The aim of the study was to investigate the effects of long-term exposure (45 days) to growth promoters: clenbuterol (CB, 1mgkg-1bw) and/or dexamethasone (DEX, 0.1mgkg-1bw), in ovarian morphology, and the possibility of recovery after the withdrawal of drug treatment. Animals were sacrificed at different days of withdrawal (W0, W5, W10, W15 and W20). Ovaries of CB treatment showed typical features of long-term administration of β-agonists at W0 with antral and atretic follicles, and the absence of corpora lutea, indicating disrupted cyclicity of rats. However, at W20 ovaries showed cyclic features with corpora lutea, antral follicles, and even recently ovulated follicles. Ovaries of CB+DEX and DEX treatments showed features of disrupted cyclicity at W0 also with cystic follicles, and fewer healthy antral follicles than those of treatment I, these same features also being observed at W20. Immunohistochemistry (IHC) of ovaries denoted that neither positive staining nor localization of steroidogenic enzymes were affected by treatments. We can conclude that treatments can disrupt the reproductive function.
AB - The aim of the study was to investigate the effects of long-term exposure (45 days) to growth promoters: clenbuterol (CB, 1mgkg-1bw) and/or dexamethasone (DEX, 0.1mgkg-1bw), in ovarian morphology, and the possibility of recovery after the withdrawal of drug treatment. Animals were sacrificed at different days of withdrawal (W0, W5, W10, W15 and W20). Ovaries of CB treatment showed typical features of long-term administration of β-agonists at W0 with antral and atretic follicles, and the absence of corpora lutea, indicating disrupted cyclicity of rats. However, at W20 ovaries showed cyclic features with corpora lutea, antral follicles, and even recently ovulated follicles. Ovaries of CB+DEX and DEX treatments showed features of disrupted cyclicity at W0 also with cystic follicles, and fewer healthy antral follicles than those of treatment I, these same features also being observed at W20. Immunohistochemistry (IHC) of ovaries denoted that neither positive staining nor localization of steroidogenic enzymes were affected by treatments. We can conclude that treatments can disrupt the reproductive function.
KW - Clenbuterol
KW - Dexamethasone
KW - Growth promoters
KW - Immunohistochemistry
KW - Ovarian morphology
KW - Rats
KW - Steroidogenic enzymes
UR - http://www.scopus.com/inward/record.url?scp=0037466548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037466548&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(02)01406-X
DO - 10.1016/S0003-2670(02)01406-X
M3 - Article
AN - SCOPUS:0037466548
VL - 483
SP - 233
EP - 240
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1-2
ER -