Effect of lipopolysaccharide infusion on gene expression of inflammatory cytokines in normal horses in vivo

Jorge Nieto, M. H. MacDonald, A. E. Poulin Braim, Monica R Aleman

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Horses are exquisitely sensitive to bacterial endotoxin and endotoxaemia is common in colic cases. In this study, gene expression of inflammatory cytokines was characterised in the blood of healthy horses following i.v. administration of lipopolysaccharide (LPS). Six horses received an LPS infusion and 6 controls received an equivalent volume of saline. Gene expression of genes encoding interleukin (IL)-1α, IL-1β, IL-6, IL-8, and tumour necrosis factor-α (TNF-α) was quantified by real-time PCR. Gene expression of all inflammatory cytokines was upregulated following administration of LPS. Interleukin-1α, IL-1β, IL-8 and TNF-α gene expression peaked at 60 min, while IL-6 expression peaked at 90 min post LPS infusion. Interleukin-1β and IL-6 messenger RNA expression levels were above the baseline values 3 h post LPS infusion, whereas IL-1α, IL-8 and TNF-α expression levels returned to baseline values by 3 h after LPS infusion. It was concluded that LPS infusion upregulated gene expression of inflammatory cytokines in the blood of healthy horses.

Original languageEnglish (US)
Pages (from-to)717-719
Number of pages3
JournalEquine Veterinary Journal
Volume41
Issue number7
DOIs
StatePublished - Jul 2009

Keywords

  • Cytokines
  • Endotoxin
  • Equine
  • Gene expression
  • Horse
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Equine

Fingerprint Dive into the research topics of 'Effect of lipopolysaccharide infusion on gene expression of inflammatory cytokines in normal horses in vivo'. Together they form a unique fingerprint.

  • Cite this