Abstract
The leucine, isoleucine, and valine patterns of blood plasma, intestinal contents, intestinal wall, liver, and skeletal muscle of rats were determined at various time intervals over a period of 12 days, during which the animals consumed ad libitum a diet containing enough leucine to cause a leucine-isoleucine and valine antagonism. Two control groups were used for comparison: one was fed a basal diet with no added leucine; the other was fed the high leucine diet supplemented with sufficient isoleucine and valine to alleviate the antagonism. The concentrations of isoleucine and valine in blood plasma from rats fed excess leucine were consistently lower than the respective concentrations in plasma from either of the control groups. Leucine concentration in plasma was greatly elevated when the dietary intake of leucine was high. The pattern for these three amino acids in muscle was similar to that in plasma. The pattern in liver was different from that in muscle or plasma and resembled that in intestinal wall and contents. It therefore appears that feeding a high leucine diet to rats results in normal isoleucine and valine concentrations in the intestine and liver, but markedly reduces concentrations of these amino acids in the skeletal muscle and systemic blood plasma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 356-361 |
| Number of pages | 6 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 113 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1966 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
Cite this
Effect of leucine-Isoleucine antagonism on the amino acid pattern of plasma and tissues of the rat. / Tannous, Raja I.; Rogers, Quinton; Harper, Alfred E.
In: Archives of Biochemistry and Biophysics, Vol. 113, No. 2, 01.01.1966, p. 356-361.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Effect of leucine-Isoleucine antagonism on the amino acid pattern of plasma and tissues of the rat
AU - Tannous, Raja I.
AU - Rogers, Quinton
AU - Harper, Alfred E.
PY - 1966/1/1
Y1 - 1966/1/1
N2 - The leucine, isoleucine, and valine patterns of blood plasma, intestinal contents, intestinal wall, liver, and skeletal muscle of rats were determined at various time intervals over a period of 12 days, during which the animals consumed ad libitum a diet containing enough leucine to cause a leucine-isoleucine and valine antagonism. Two control groups were used for comparison: one was fed a basal diet with no added leucine; the other was fed the high leucine diet supplemented with sufficient isoleucine and valine to alleviate the antagonism. The concentrations of isoleucine and valine in blood plasma from rats fed excess leucine were consistently lower than the respective concentrations in plasma from either of the control groups. Leucine concentration in plasma was greatly elevated when the dietary intake of leucine was high. The pattern for these three amino acids in muscle was similar to that in plasma. The pattern in liver was different from that in muscle or plasma and resembled that in intestinal wall and contents. It therefore appears that feeding a high leucine diet to rats results in normal isoleucine and valine concentrations in the intestine and liver, but markedly reduces concentrations of these amino acids in the skeletal muscle and systemic blood plasma.
AB - The leucine, isoleucine, and valine patterns of blood plasma, intestinal contents, intestinal wall, liver, and skeletal muscle of rats were determined at various time intervals over a period of 12 days, during which the animals consumed ad libitum a diet containing enough leucine to cause a leucine-isoleucine and valine antagonism. Two control groups were used for comparison: one was fed a basal diet with no added leucine; the other was fed the high leucine diet supplemented with sufficient isoleucine and valine to alleviate the antagonism. The concentrations of isoleucine and valine in blood plasma from rats fed excess leucine were consistently lower than the respective concentrations in plasma from either of the control groups. Leucine concentration in plasma was greatly elevated when the dietary intake of leucine was high. The pattern for these three amino acids in muscle was similar to that in plasma. The pattern in liver was different from that in muscle or plasma and resembled that in intestinal wall and contents. It therefore appears that feeding a high leucine diet to rats results in normal isoleucine and valine concentrations in the intestine and liver, but markedly reduces concentrations of these amino acids in the skeletal muscle and systemic blood plasma.
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UR - http://www.scopus.com/inward/citedby.url?scp=0013882690&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(66)90197-4
DO - 10.1016/0003-9861(66)90197-4
M3 - Article
C2 - 5941193
AN - SCOPUS:0013882690
VL - 113
SP - 356
EP - 361
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 2
ER -