Abstract
Mouse bone marrow hematopoietic stem cells were isolated from mouse femur bone and cultured in RPMI 1640 supplemented medium with 20 units/ml of the purified T-cell lymphokine, interleukin 3 (IL-3). IL-3 was uniquely able to induce the proliferation and differentiation of mature mast cells in vitro. The sparse granulation of the bone marrow-derived mast cells (BMMC) can be seen by day 5, progressing to definable mast cells by day 7, the mast cells appear morphologically mature and comprise a 96% pure population after 14 days of the culture. The monocytes/macrophages, eosinophils and neutrophils disappeared by day 9. After 4 weeks of tissue culture, mast cells are fully mature and completely granulated at 98% cell purity. The BMMC are mononuclear, oval or round in shape and appear smaller than rat peritoneal mast cells. BMMC are stable over 3-5 months in conditioned medium. The homogeneous mast cell population possesses membrane receptors and mediators, such as histamine in their metachromatic granules. The histamine content of BMMC in culture between 2 to 4 weeks rose from 1.43 to 1.82 pg/cell. Moreover, the percentage of histamine release caused by 0.1 μM and 1.0 μM ionophore A23187 was 15% and 35%, respectively. By contrast, the histamine releasing activity of 0.01% and 0.001% compound 48/80 were 12±2% and 59±7% respectively. The granular density, histamine content and histamine release activity of BMMC are different from that of peritoneal mast cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 197-203 |
| Number of pages | 7 |
| Journal | Agents and Actions |
| Volume | 31 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Nov 1990 |
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ASJC Scopus subject areas
- Toxicology
- Pharmacology (medical)
Cite this
Effect of interleukin 3 on the differentiation and histamine content of cultured bone marrow mast cells. / Chiu, H. F.; Burrall, Barbara A.
In: Agents and Actions, Vol. 31, No. 3-4, 11.1990, p. 197-203.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of interleukin 3 on the differentiation and histamine content of cultured bone marrow mast cells
AU - Chiu, H. F.
AU - Burrall, Barbara A
PY - 1990/11
Y1 - 1990/11
N2 - Mouse bone marrow hematopoietic stem cells were isolated from mouse femur bone and cultured in RPMI 1640 supplemented medium with 20 units/ml of the purified T-cell lymphokine, interleukin 3 (IL-3). IL-3 was uniquely able to induce the proliferation and differentiation of mature mast cells in vitro. The sparse granulation of the bone marrow-derived mast cells (BMMC) can be seen by day 5, progressing to definable mast cells by day 7, the mast cells appear morphologically mature and comprise a 96% pure population after 14 days of the culture. The monocytes/macrophages, eosinophils and neutrophils disappeared by day 9. After 4 weeks of tissue culture, mast cells are fully mature and completely granulated at 98% cell purity. The BMMC are mononuclear, oval or round in shape and appear smaller than rat peritoneal mast cells. BMMC are stable over 3-5 months in conditioned medium. The homogeneous mast cell population possesses membrane receptors and mediators, such as histamine in their metachromatic granules. The histamine content of BMMC in culture between 2 to 4 weeks rose from 1.43 to 1.82 pg/cell. Moreover, the percentage of histamine release caused by 0.1 μM and 1.0 μM ionophore A23187 was 15% and 35%, respectively. By contrast, the histamine releasing activity of 0.01% and 0.001% compound 48/80 were 12±2% and 59±7% respectively. The granular density, histamine content and histamine release activity of BMMC are different from that of peritoneal mast cells.
AB - Mouse bone marrow hematopoietic stem cells were isolated from mouse femur bone and cultured in RPMI 1640 supplemented medium with 20 units/ml of the purified T-cell lymphokine, interleukin 3 (IL-3). IL-3 was uniquely able to induce the proliferation and differentiation of mature mast cells in vitro. The sparse granulation of the bone marrow-derived mast cells (BMMC) can be seen by day 5, progressing to definable mast cells by day 7, the mast cells appear morphologically mature and comprise a 96% pure population after 14 days of the culture. The monocytes/macrophages, eosinophils and neutrophils disappeared by day 9. After 4 weeks of tissue culture, mast cells are fully mature and completely granulated at 98% cell purity. The BMMC are mononuclear, oval or round in shape and appear smaller than rat peritoneal mast cells. BMMC are stable over 3-5 months in conditioned medium. The homogeneous mast cell population possesses membrane receptors and mediators, such as histamine in their metachromatic granules. The histamine content of BMMC in culture between 2 to 4 weeks rose from 1.43 to 1.82 pg/cell. Moreover, the percentage of histamine release caused by 0.1 μM and 1.0 μM ionophore A23187 was 15% and 35%, respectively. By contrast, the histamine releasing activity of 0.01% and 0.001% compound 48/80 were 12±2% and 59±7% respectively. The granular density, histamine content and histamine release activity of BMMC are different from that of peritoneal mast cells.
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U2 - 10.1007/BF01997608
DO - 10.1007/BF01997608
M3 - Article
C2 - 1707584
AN - SCOPUS:0025203057
VL - 31
SP - 197
EP - 203
JO - Inflammation Research
JF - Inflammation Research
SN - 1023-3830
IS - 3-4
ER -