Effect of Insulin and Prolactin on Acyltransferase Activities in MAC-T Bovine Mammary Cells

L. Z. Morand, J. N. Morand, R. Matson, J. B. German

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The enzymatic activities of sn-glycerol-3-phosphate acyltransferase and lysophosphatidate acyltransferase were investigated in microsomal fractions prepared from MAC-T cells from bovine mammary gland and from FTO-2B cells from rat liver. In both cell lines, sn-glycerol-3-phosphate acyltransferase exhibited similar rates of palmitate and oleate incorporation. However, lysophosphatidate acyltransferase activity in MAC-T cells had a 2.8-fold greater rate of palmitate incorporation than of oleate incorporation. In FTO-2B cells, there was a 1.4-fold greater rate of oleate incorporation than of palmitate incorporation. FTO-2B and MAC-T cells displayed acyltransferase activities that were consistent with liver and mammary tissues, respectively. The acyltransferases were examined from FTO-2B and MAC-T cells that were treated with insulin and prolactin. Insulin suppressed both acyltransferase activities in FTO-2B cells, and prolactin had a stimulatory effect; however, these effects were very small. In contrast, insulin and prolactin had a stimulatory effect on both acyltransferase activities in MAC-T cells; prolactin elicited the largest effect. Treatment of MAC-T cells with cycloheximide inhibited the stimulatory effect of prolactin on acyltransferase activities.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalJournal of Dairy Science
Volume81
Issue number1
StatePublished - Jan 1998

Keywords

  • Acyltransferase
  • Lactogenic hormones
  • MAC-T cells

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • veterinary(all)

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    Morand, L. Z., Morand, J. N., Matson, R., & German, J. B. (1998). Effect of Insulin and Prolactin on Acyltransferase Activities in MAC-T Bovine Mammary Cells. Journal of Dairy Science, 81(1), 100-106.