Abstract
The enzymatic activities of sn-glycerol-3-phosphate acyltransferase and lysophosphatidate acyltransferase were investigated in microsomal fractions prepared from MAC-T cells from bovine mammary gland and from FTO-2B cells from rat liver. In both cell lines, sn-glycerol-3-phosphate acyltransferase exhibited similar rates of palmitate and oleate incorporation. However, lysophosphatidate acyltransferase activity in MAC-T cells had a 2.8-fold greater rate of palmitate incorporation than of oleate incorporation. In FTO-2B cells, there was a 1.4-fold greater rate of oleate incorporation than of palmitate incorporation. FTO-2B and MAC-T cells displayed acyltransferase activities that were consistent with liver and mammary tissues, respectively. The acyltransferases were examined from FTO-2B and MAC-T cells that were treated with insulin and prolactin. Insulin suppressed both acyltransferase activities in FTO-2B cells, and prolactin had a stimulatory effect; however, these effects were very small. In contrast, insulin and prolactin had a stimulatory effect on both acyltransferase activities in MAC-T cells; prolactin elicited the largest effect. Treatment of MAC-T cells with cycloheximide inhibited the stimulatory effect of prolactin on acyltransferase activities.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 100-106 |
| Number of pages | 7 |
| Journal | Journal of Dairy Science |
| Volume | 81 |
| Issue number | 1 |
| State | Published - Jan 1998 |
Fingerprint
Keywords
- Acyltransferase
- Lactogenic hormones
- MAC-T cells
ASJC Scopus subject areas
- Animal Science and Zoology
- Food Science
- veterinary(all)
Cite this
Effect of Insulin and Prolactin on Acyltransferase Activities in MAC-T Bovine Mammary Cells. / Morand, L. Z.; Morand, J. N.; Matson, R.; German, J. B.
In: Journal of Dairy Science, Vol. 81, No. 1, 01.1998, p. 100-106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Insulin and Prolactin on Acyltransferase Activities in MAC-T Bovine Mammary Cells
AU - Morand, L. Z.
AU - Morand, J. N.
AU - Matson, R.
AU - German, J. B.
PY - 1998/1
Y1 - 1998/1
N2 - The enzymatic activities of sn-glycerol-3-phosphate acyltransferase and lysophosphatidate acyltransferase were investigated in microsomal fractions prepared from MAC-T cells from bovine mammary gland and from FTO-2B cells from rat liver. In both cell lines, sn-glycerol-3-phosphate acyltransferase exhibited similar rates of palmitate and oleate incorporation. However, lysophosphatidate acyltransferase activity in MAC-T cells had a 2.8-fold greater rate of palmitate incorporation than of oleate incorporation. In FTO-2B cells, there was a 1.4-fold greater rate of oleate incorporation than of palmitate incorporation. FTO-2B and MAC-T cells displayed acyltransferase activities that were consistent with liver and mammary tissues, respectively. The acyltransferases were examined from FTO-2B and MAC-T cells that were treated with insulin and prolactin. Insulin suppressed both acyltransferase activities in FTO-2B cells, and prolactin had a stimulatory effect; however, these effects were very small. In contrast, insulin and prolactin had a stimulatory effect on both acyltransferase activities in MAC-T cells; prolactin elicited the largest effect. Treatment of MAC-T cells with cycloheximide inhibited the stimulatory effect of prolactin on acyltransferase activities.
AB - The enzymatic activities of sn-glycerol-3-phosphate acyltransferase and lysophosphatidate acyltransferase were investigated in microsomal fractions prepared from MAC-T cells from bovine mammary gland and from FTO-2B cells from rat liver. In both cell lines, sn-glycerol-3-phosphate acyltransferase exhibited similar rates of palmitate and oleate incorporation. However, lysophosphatidate acyltransferase activity in MAC-T cells had a 2.8-fold greater rate of palmitate incorporation than of oleate incorporation. In FTO-2B cells, there was a 1.4-fold greater rate of oleate incorporation than of palmitate incorporation. FTO-2B and MAC-T cells displayed acyltransferase activities that were consistent with liver and mammary tissues, respectively. The acyltransferases were examined from FTO-2B and MAC-T cells that were treated with insulin and prolactin. Insulin suppressed both acyltransferase activities in FTO-2B cells, and prolactin had a stimulatory effect; however, these effects were very small. In contrast, insulin and prolactin had a stimulatory effect on both acyltransferase activities in MAC-T cells; prolactin elicited the largest effect. Treatment of MAC-T cells with cycloheximide inhibited the stimulatory effect of prolactin on acyltransferase activities.
KW - Acyltransferase
KW - Lactogenic hormones
KW - MAC-T cells
UR - http://www.scopus.com/inward/record.url?scp=0031607268&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031607268&partnerID=8YFLogxK
M3 - Article
C2 - 9493084
AN - SCOPUS:0031607268
VL - 81
SP - 100
EP - 106
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 1
ER -