Effect of in utero and postnatal exposure to environmental tobacco smoke on the developmental expression of pulmonary cytochrome P450 monooxygenases

Chanhung Z. Lee, Fred H. Royce, Michael S. Denison, Kent E Pinkerton

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pulmonary cytochrome P450 monooxygenases metabolize xenobiotic chemicals, including those found in environmental tobacco smoke (ETS). Exposure to ETS beginning at birth has been shown to induce the P450 CYP1A1 by seven days of life. The effects of perinatal exposure to ETS of the rat lung on the expression of CYP1A1,1B1, 2B1, and NADPH cytochrome P450 reductase were measured using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Timed pregnant dams and their pups were exposed to aged and diluted sidestream cigarette smoke (ADSS) as a surrogate for ETS for four hours/ day from gestational day 5 through postnatal day 21. For all genes analyzed, mRNA could be detected in the fetal lung beginning at gestational day 17 but were not altered by ADSS. In contrast, intraperitoneal injection of dams with β-naphthoflavone significantly elevated both CYP1A1 and 1B1 at gestational day 21, indicating that these genes are inducible. Continued exposure to ADSS significantly induced CYP1A1 but not other P450 genes as early as one day after birth.. We conclude that (1) ADSS induces pulmonary CYP1A1 in the first day of life; (2) fetal cytochrome P450 genes are not in-duced by maternal exposure to ADSS; and (3) in the fetal lung, CYP1A1 and 1B1 can be induced by β-naphthoflavone.

Original languageEnglish (US)
Pages (from-to)121-130
Number of pages10
JournalJournal of Biochemical and Molecular Toxicology
Volume14
Issue number3
StatePublished - 2000

Fingerprint

Tobacco
Environmental Exposure
Mixed Function Oxygenases
Smoke
Cytochrome P-450 Enzyme System
Cytochrome P-450 CYP1A1
Lung
Tobacco Products
Genes
Dams
Parturition
Aryl Hydrocarbon Hydroxylases
Maternal Exposure
NADPH-Ferrihemoprotein Reductase
Polymerase chain reaction
RNA-Directed DNA Polymerase
Xenobiotics
Intraperitoneal Injections
Reverse Transcriptase Polymerase Chain Reaction
Rats

Keywords

  • Aged and Diluted Sidestream Smoke
  • Lung Development
  • P450
  • Rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Effect of in utero and postnatal exposure to environmental tobacco smoke on the developmental expression of pulmonary cytochrome P450 monooxygenases. / Lee, Chanhung Z.; Royce, Fred H.; Denison, Michael S.; Pinkerton, Kent E.

In: Journal of Biochemical and Molecular Toxicology, Vol. 14, No. 3, 2000, p. 121-130.

Research output: Contribution to journalArticle

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