Effect of ICSI on gene expression and development of mouse preimplantation embryos

G. Giritharan, M. W. Li, F. De Sebastiano, F. J. Esteban, J. A. Horcajadas, Kevin C K Lloyd, A. Donjacour, E. Maltepe, P. F. Rinaudo

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

In vitro culture (IVC) and IVF of preimplantation mouse embryos are associated with changes in gene expression. It is however not known whether ICSI has additional effects on the transcriptome of mouse blastocysts. Methods We compared gene expression and development of mouse blastocysts produced by ICSI and cultured in Whitten's medium (ICSIWM) or KSOM medium with amino acids (ICSIKSOMaa) with control blastocysts flushed out of the uterus on post coital Day 3.5 (in vivo). In addition, we compared gene expression in embryos generated by IVF or ICSI using WM. Global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip. Results Blastocysts from ICSI fertilization have a reduction in the number of trophoblastic and inner cell mass cells compared with embryos generated in vivo. Approximately 1000 genes are differentially expressed between ICSI blastocyst and in vivo blastocysts; proliferation, apoptosis and morphogenetic pathways are the most common pathways altered after IVC. Unexpectedly, expression of only 41 genes was significantly different between embryo cultured in suboptimal conditions (WM) or optimal conditions (KSOMaa). Conclusions Our results suggest that fertilization by ICSI may play a more important role in shaping the transcriptome of the developing mouse embryo than the culture media used.

Original languageEnglish (US)
Pages (from-to)3012-3024
Number of pages13
JournalHuman Reproduction
Volume25
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

Intracytoplasmic Sperm Injections
Blastocyst
Embryonic Development
Gene Expression
Embryonic Structures
Transcriptome
Fertilization
Genes
Uterus
Culture Media
Apoptosis
Amino Acids

Keywords

  • culture medium
  • embryos
  • gene expression
  • ICSI
  • microarray

ASJC Scopus subject areas

  • Rehabilitation
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Giritharan, G., Li, M. W., De Sebastiano, F., Esteban, F. J., Horcajadas, J. A., Lloyd, K. C. K., ... Rinaudo, P. F. (2010). Effect of ICSI on gene expression and development of mouse preimplantation embryos. Human Reproduction, 25(12), 3012-3024. https://doi.org/10.1093/humrep/deq266

Effect of ICSI on gene expression and development of mouse preimplantation embryos. / Giritharan, G.; Li, M. W.; De Sebastiano, F.; Esteban, F. J.; Horcajadas, J. A.; Lloyd, Kevin C K; Donjacour, A.; Maltepe, E.; Rinaudo, P. F.

In: Human Reproduction, Vol. 25, No. 12, 12.2010, p. 3012-3024.

Research output: Contribution to journalArticle

Giritharan, G, Li, MW, De Sebastiano, F, Esteban, FJ, Horcajadas, JA, Lloyd, KCK, Donjacour, A, Maltepe, E & Rinaudo, PF 2010, 'Effect of ICSI on gene expression and development of mouse preimplantation embryos', Human Reproduction, vol. 25, no. 12, pp. 3012-3024. https://doi.org/10.1093/humrep/deq266
Giritharan G, Li MW, De Sebastiano F, Esteban FJ, Horcajadas JA, Lloyd KCK et al. Effect of ICSI on gene expression and development of mouse preimplantation embryos. Human Reproduction. 2010 Dec;25(12):3012-3024. https://doi.org/10.1093/humrep/deq266
Giritharan, G. ; Li, M. W. ; De Sebastiano, F. ; Esteban, F. J. ; Horcajadas, J. A. ; Lloyd, Kevin C K ; Donjacour, A. ; Maltepe, E. ; Rinaudo, P. F. / Effect of ICSI on gene expression and development of mouse preimplantation embryos. In: Human Reproduction. 2010 ; Vol. 25, No. 12. pp. 3012-3024.
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