Effect of hypoinsulinemia on growth in the fetal rabbit

D. G. Chaffin, R. M. Clark, D. McCracken, Anthony F Philipps

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Insulin is an important regulatory hormone in the control of fetal growth. In a fetal rabbit model, a non-ruminant species, the effects of insulin deprivation on glucose, growth and protein metabolism were studied. The fetuses of 10 pregnant New Zealand white rabbits in one uterine horn received a subcutaneous injection on day 25 of gestation (term = 30 days) of 0.1 mg streptozotocin (STZ)/gm fetal weight. The fetuses in the opposite horn received a sham injection of buffer. The dams were then killed on day 29. The fetal insulin concentration was depressed by 38%, and the serum glucose concentration was elevated by 28% in animals given STZ when compared to control animals. Fetal weights, carcass weights and skeletal growth, as measured by crown-rump length and tibial length of STZ-treated fetuses, were significantly reduced by 7-13%. However, organ weights were not significantly different, except for the kidneys which were 17% lighter. Protein and mineral contents of the carcasses were also reduced compared to the control fetuses. Thus, insulin deprivation in fetal rabbits results in significant growth impairment in a pattern similar to that of human asymmetric intrauterine growth retardation.

Original languageEnglish (US)
Pages (from-to)186-193
Number of pages8
JournalBiology of the Neonate
Volume67
Issue number3
StatePublished - 1995
Externally publishedYes

Keywords

  • Fetal growth
  • Insulin
  • Streptozotocin

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

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  • Cite this

    Chaffin, D. G., Clark, R. M., McCracken, D., & Philipps, A. F. (1995). Effect of hypoinsulinemia on growth in the fetal rabbit. Biology of the Neonate, 67(3), 186-193.