Effect of hydroxylated carotenoid deficiency on ABA accumulation in Arabidopsis

Li Tian, Dean DellaPenna, Jan A D Zeevaart

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Abscisic acid (ABA) is a sesquiterpene compound (C15) derived from C40 carotenoids. The immediate carotenoid precursors for ABA biosynthesis, 9-cis-violaxanthin and 9′-cis-neoxanthin, are produced from β-carotene by a series of hydroxylation, epoxidation, and isomerization reactions. Carotenoid hydroxylase deficient mutants contain severely reduced levels of violaxanthin and neoxanthin (<20% of wild type level) and provide a unique system to correlate carotenoid substrate availability and ABA production in photosynthetic tissues under non-stressed conditions. Quantitative measurements indicated that ABA levels in the carotenoid hydroxylase mutants are reduced nearly 50% compared to the wild type plants under non-stressed conditions. When drought-stressed, wild type plants showed up to a 17-fold increase in ABA levels, while ABA levels in the carotenoid hydroxylase mutants were only increased 6- to 7-fold (25% of wild type drought-stressed ABA levels). Expression of AtNCED3 (Arabidopsis thaliana nine-cis-epoxycarotenoid dioxygenase 3, the rate-limiting activity for ABA biosynthesis) was induced in the carotenoid hydroxylase mutants, but to a lesser extent than the 40-fold increase in wild type plants. Therefore, the reduced ABA accumulation in response to drought-stress is at least partially due to the attenuated increase in AtNCED3 gene expression in the carotenoid hydroxylase mutants. The remaining violaxanthin and neoxanthin in the carotenoid hydroxylase mutants can not be converted into ABA, indicating that there is probably a separate pool of violaxanthin and neoxanthin that is not accessible to the cleavage enzymes, because it is sequestered in the light-harvesting complexes.

Original languageEnglish (US)
Pages (from-to)314-320
Number of pages7
JournalPhysiologia Plantarum
Volume122
Issue number3
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Abscisic Acid
Carotenoids
Arabidopsis
abscisic acid
carotenoids
Mixed Function Oxygenases
neoxanthin
violaxanthin
mutants
Droughts
drought
biosynthesis
Dioxygenases
light harvesting complex
Sesquiterpenes
isomerization
hydroxylation
carotenes
Hydroxylation
sesquiterpenoids

ASJC Scopus subject areas

  • Plant Science

Cite this

Effect of hydroxylated carotenoid deficiency on ABA accumulation in Arabidopsis. / Tian, Li; DellaPenna, Dean; Zeevaart, Jan A D.

In: Physiologia Plantarum, Vol. 122, No. 3, 11.2004, p. 314-320.

Research output: Contribution to journalArticle

Tian, Li ; DellaPenna, Dean ; Zeevaart, Jan A D. / Effect of hydroxylated carotenoid deficiency on ABA accumulation in Arabidopsis. In: Physiologia Plantarum. 2004 ; Vol. 122, No. 3. pp. 314-320.
@article{7a61b3b970fc4146a46ec0875e18a5c6,
title = "Effect of hydroxylated carotenoid deficiency on ABA accumulation in Arabidopsis",
abstract = "Abscisic acid (ABA) is a sesquiterpene compound (C15) derived from C40 carotenoids. The immediate carotenoid precursors for ABA biosynthesis, 9-cis-violaxanthin and 9′-cis-neoxanthin, are produced from β-carotene by a series of hydroxylation, epoxidation, and isomerization reactions. Carotenoid hydroxylase deficient mutants contain severely reduced levels of violaxanthin and neoxanthin (<20{\%} of wild type level) and provide a unique system to correlate carotenoid substrate availability and ABA production in photosynthetic tissues under non-stressed conditions. Quantitative measurements indicated that ABA levels in the carotenoid hydroxylase mutants are reduced nearly 50{\%} compared to the wild type plants under non-stressed conditions. When drought-stressed, wild type plants showed up to a 17-fold increase in ABA levels, while ABA levels in the carotenoid hydroxylase mutants were only increased 6- to 7-fold (25{\%} of wild type drought-stressed ABA levels). Expression of AtNCED3 (Arabidopsis thaliana nine-cis-epoxycarotenoid dioxygenase 3, the rate-limiting activity for ABA biosynthesis) was induced in the carotenoid hydroxylase mutants, but to a lesser extent than the 40-fold increase in wild type plants. Therefore, the reduced ABA accumulation in response to drought-stress is at least partially due to the attenuated increase in AtNCED3 gene expression in the carotenoid hydroxylase mutants. The remaining violaxanthin and neoxanthin in the carotenoid hydroxylase mutants can not be converted into ABA, indicating that there is probably a separate pool of violaxanthin and neoxanthin that is not accessible to the cleavage enzymes, because it is sequestered in the light-harvesting complexes.",
author = "Li Tian and Dean DellaPenna and Zeevaart, {Jan A D}",
year = "2004",
month = "11",
doi = "10.1111/j.1399-3054.2004.00409.x",
language = "English (US)",
volume = "122",
pages = "314--320",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Effect of hydroxylated carotenoid deficiency on ABA accumulation in Arabidopsis

AU - Tian, Li

AU - DellaPenna, Dean

AU - Zeevaart, Jan A D

PY - 2004/11

Y1 - 2004/11

N2 - Abscisic acid (ABA) is a sesquiterpene compound (C15) derived from C40 carotenoids. The immediate carotenoid precursors for ABA biosynthesis, 9-cis-violaxanthin and 9′-cis-neoxanthin, are produced from β-carotene by a series of hydroxylation, epoxidation, and isomerization reactions. Carotenoid hydroxylase deficient mutants contain severely reduced levels of violaxanthin and neoxanthin (<20% of wild type level) and provide a unique system to correlate carotenoid substrate availability and ABA production in photosynthetic tissues under non-stressed conditions. Quantitative measurements indicated that ABA levels in the carotenoid hydroxylase mutants are reduced nearly 50% compared to the wild type plants under non-stressed conditions. When drought-stressed, wild type plants showed up to a 17-fold increase in ABA levels, while ABA levels in the carotenoid hydroxylase mutants were only increased 6- to 7-fold (25% of wild type drought-stressed ABA levels). Expression of AtNCED3 (Arabidopsis thaliana nine-cis-epoxycarotenoid dioxygenase 3, the rate-limiting activity for ABA biosynthesis) was induced in the carotenoid hydroxylase mutants, but to a lesser extent than the 40-fold increase in wild type plants. Therefore, the reduced ABA accumulation in response to drought-stress is at least partially due to the attenuated increase in AtNCED3 gene expression in the carotenoid hydroxylase mutants. The remaining violaxanthin and neoxanthin in the carotenoid hydroxylase mutants can not be converted into ABA, indicating that there is probably a separate pool of violaxanthin and neoxanthin that is not accessible to the cleavage enzymes, because it is sequestered in the light-harvesting complexes.

AB - Abscisic acid (ABA) is a sesquiterpene compound (C15) derived from C40 carotenoids. The immediate carotenoid precursors for ABA biosynthesis, 9-cis-violaxanthin and 9′-cis-neoxanthin, are produced from β-carotene by a series of hydroxylation, epoxidation, and isomerization reactions. Carotenoid hydroxylase deficient mutants contain severely reduced levels of violaxanthin and neoxanthin (<20% of wild type level) and provide a unique system to correlate carotenoid substrate availability and ABA production in photosynthetic tissues under non-stressed conditions. Quantitative measurements indicated that ABA levels in the carotenoid hydroxylase mutants are reduced nearly 50% compared to the wild type plants under non-stressed conditions. When drought-stressed, wild type plants showed up to a 17-fold increase in ABA levels, while ABA levels in the carotenoid hydroxylase mutants were only increased 6- to 7-fold (25% of wild type drought-stressed ABA levels). Expression of AtNCED3 (Arabidopsis thaliana nine-cis-epoxycarotenoid dioxygenase 3, the rate-limiting activity for ABA biosynthesis) was induced in the carotenoid hydroxylase mutants, but to a lesser extent than the 40-fold increase in wild type plants. Therefore, the reduced ABA accumulation in response to drought-stress is at least partially due to the attenuated increase in AtNCED3 gene expression in the carotenoid hydroxylase mutants. The remaining violaxanthin and neoxanthin in the carotenoid hydroxylase mutants can not be converted into ABA, indicating that there is probably a separate pool of violaxanthin and neoxanthin that is not accessible to the cleavage enzymes, because it is sequestered in the light-harvesting complexes.

UR - http://www.scopus.com/inward/record.url?scp=7444265319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7444265319&partnerID=8YFLogxK

U2 - 10.1111/j.1399-3054.2004.00409.x

DO - 10.1111/j.1399-3054.2004.00409.x

M3 - Article

VL - 122

SP - 314

EP - 320

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 3

ER -