Effect of esophageal ligation on the development of fetal rabbit intestinal lactase

Terry L. Buchmiller, Jeffrey Gregg, Francisco A. Rivera, Jared M. Diamond, Eric W. Fonkalsrud

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To investigate the effect of normal fetal swallowing and amniotic fluid ingestion on small intestinal disaccharidase development, 13 pregnant New Zealand White rabbits underwent operation on day 24 of a normal 31-day gestation. The right ovarian fetus in the bicomuate uterus underwent esophageal ligation (EL), while the contralateral left fetus underwent cervical exploration only, and served as the control (C). Rabbits were sacrificed on gestational day 31, fetal somatic measurements obtained, and the midjejunum removed for determination of disaccharidase activity and protein content. There was one maternal death, and 9 of 12 fetal pairs survived the entire study period (75%). Results are reported as mean ± SEM, analyzed by two-tailed Student's t testing with P < .05 being considered significant. Fetal weight was decreased in EL (48.6 ± 2.7 g) versus C (51.4 ± 3.2 g) (P = .06). Small intestinal length decreased in EL (49.2 ± 2.0 cm) versus C (54.9 ± 1.1 cm) (P = .01). Midjejunal protein content (mg/mL homogenate) was also significantly decreased in EL (38.4 ± 3.4) versus C (46.2 ± 3.7) (P = .05). Sucrase activity was not detectable in either group. Lactase activity in jejunal mucosa was not effected when expressed as units of enzyme per milliliter of homogenate (EL = 0.357 ± 0.03 v C = 0.373 ± 0.04; P = .70) and units enzyme per gram of protein (EL = 38.8 ± 4.2 v C = 34.2 ± 4.6; P = .44). We have confirmed previous studies demonstrating decreases in somatic growth, small intestinal length, and mucosal nutrient transport in rabbit fetuses following esophageal ligation. The present study demonstrates, in contrast, that the brush border disaccharidase lactase develops independently of the normal fetal swallowing mechanism with the concomitant ingestion of amniotic fluid. Implications for infants with intestinal atresias are under investigation.

Original languageEnglish (US)
Pages (from-to)1473-1477
Number of pages5
JournalJournal of Pediatric Surgery
Volume28
Issue number11
DOIs
StatePublished - 1993

Fingerprint

Lactase
Fetal Development
Ligation
Rabbits
Disaccharidases
Fetus
Amniotic Fluid
Deglutition
Eating
Intestinal Atresia
Sucrase
Maternal Death
Fetal Weight
Proteins
Enzymes
Microvilli
Uterus
Mucous Membrane
Students
Food

Keywords

  • esophageal atresia
  • Rabbit, fetal, intestinal lactase

ASJC Scopus subject areas

  • Surgery

Cite this

Effect of esophageal ligation on the development of fetal rabbit intestinal lactase. / Buchmiller, Terry L.; Gregg, Jeffrey; Rivera, Francisco A.; Diamond, Jared M.; Fonkalsrud, Eric W.

In: Journal of Pediatric Surgery, Vol. 28, No. 11, 1993, p. 1473-1477.

Research output: Contribution to journalArticle

Buchmiller, Terry L. ; Gregg, Jeffrey ; Rivera, Francisco A. ; Diamond, Jared M. ; Fonkalsrud, Eric W. / Effect of esophageal ligation on the development of fetal rabbit intestinal lactase. In: Journal of Pediatric Surgery. 1993 ; Vol. 28, No. 11. pp. 1473-1477.
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AB - To investigate the effect of normal fetal swallowing and amniotic fluid ingestion on small intestinal disaccharidase development, 13 pregnant New Zealand White rabbits underwent operation on day 24 of a normal 31-day gestation. The right ovarian fetus in the bicomuate uterus underwent esophageal ligation (EL), while the contralateral left fetus underwent cervical exploration only, and served as the control (C). Rabbits were sacrificed on gestational day 31, fetal somatic measurements obtained, and the midjejunum removed for determination of disaccharidase activity and protein content. There was one maternal death, and 9 of 12 fetal pairs survived the entire study period (75%). Results are reported as mean ± SEM, analyzed by two-tailed Student's t testing with P < .05 being considered significant. Fetal weight was decreased in EL (48.6 ± 2.7 g) versus C (51.4 ± 3.2 g) (P = .06). Small intestinal length decreased in EL (49.2 ± 2.0 cm) versus C (54.9 ± 1.1 cm) (P = .01). Midjejunal protein content (mg/mL homogenate) was also significantly decreased in EL (38.4 ± 3.4) versus C (46.2 ± 3.7) (P = .05). Sucrase activity was not detectable in either group. Lactase activity in jejunal mucosa was not effected when expressed as units of enzyme per milliliter of homogenate (EL = 0.357 ± 0.03 v C = 0.373 ± 0.04; P = .70) and units enzyme per gram of protein (EL = 38.8 ± 4.2 v C = 34.2 ± 4.6; P = .44). We have confirmed previous studies demonstrating decreases in somatic growth, small intestinal length, and mucosal nutrient transport in rabbit fetuses following esophageal ligation. The present study demonstrates, in contrast, that the brush border disaccharidase lactase develops independently of the normal fetal swallowing mechanism with the concomitant ingestion of amniotic fluid. Implications for infants with intestinal atresias are under investigation.

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