Abstract
To investigate the effect of normal fetal swallowing and amniotic fluid ingestion on small intestinal disaccharidase development, 13 pregnant New Zealand White rabbits underwent operation on day 24 of a normal 31-day gestation. The right ovarian fetus in the bicomuate uterus underwent esophageal ligation (EL), while the contralateral left fetus underwent cervical exploration only, and served as the control (C). Rabbits were sacrificed on gestational day 31, fetal somatic measurements obtained, and the midjejunum removed for determination of disaccharidase activity and protein content. There was one maternal death, and 9 of 12 fetal pairs survived the entire study period (75%). Results are reported as mean ± SEM, analyzed by two-tailed Student's t testing with P < .05 being considered significant. Fetal weight was decreased in EL (48.6 ± 2.7 g) versus C (51.4 ± 3.2 g) (P = .06). Small intestinal length decreased in EL (49.2 ± 2.0 cm) versus C (54.9 ± 1.1 cm) (P = .01). Midjejunal protein content (mg/mL homogenate) was also significantly decreased in EL (38.4 ± 3.4) versus C (46.2 ± 3.7) (P = .05). Sucrase activity was not detectable in either group. Lactase activity in jejunal mucosa was not effected when expressed as units of enzyme per milliliter of homogenate (EL = 0.357 ± 0.03 v C = 0.373 ± 0.04; P = .70) and units enzyme per gram of protein (EL = 38.8 ± 4.2 v C = 34.2 ± 4.6; P = .44). We have confirmed previous studies demonstrating decreases in somatic growth, small intestinal length, and mucosal nutrient transport in rabbit fetuses following esophageal ligation. The present study demonstrates, in contrast, that the brush border disaccharidase lactase develops independently of the normal fetal swallowing mechanism with the concomitant ingestion of amniotic fluid. Implications for infants with intestinal atresias are under investigation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1473-1477 |
| Number of pages | 5 |
| Journal | Journal of Pediatric Surgery |
| Volume | 28 |
| Issue number | 11 |
| DOIs | |
| State | Published - 1993 |
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Keywords
- esophageal atresia
- Rabbit, fetal, intestinal lactase
ASJC Scopus subject areas
- Surgery
Cite this
Effect of esophageal ligation on the development of fetal rabbit intestinal lactase. / Buchmiller, Terry L.; Gregg, Jeffrey; Rivera, Francisco A.; Diamond, Jared M.; Fonkalsrud, Eric W.
In: Journal of Pediatric Surgery, Vol. 28, No. 11, 1993, p. 1473-1477.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of esophageal ligation on the development of fetal rabbit intestinal lactase
AU - Buchmiller, Terry L.
AU - Gregg, Jeffrey
AU - Rivera, Francisco A.
AU - Diamond, Jared M.
AU - Fonkalsrud, Eric W.
PY - 1993
Y1 - 1993
N2 - To investigate the effect of normal fetal swallowing and amniotic fluid ingestion on small intestinal disaccharidase development, 13 pregnant New Zealand White rabbits underwent operation on day 24 of a normal 31-day gestation. The right ovarian fetus in the bicomuate uterus underwent esophageal ligation (EL), while the contralateral left fetus underwent cervical exploration only, and served as the control (C). Rabbits were sacrificed on gestational day 31, fetal somatic measurements obtained, and the midjejunum removed for determination of disaccharidase activity and protein content. There was one maternal death, and 9 of 12 fetal pairs survived the entire study period (75%). Results are reported as mean ± SEM, analyzed by two-tailed Student's t testing with P < .05 being considered significant. Fetal weight was decreased in EL (48.6 ± 2.7 g) versus C (51.4 ± 3.2 g) (P = .06). Small intestinal length decreased in EL (49.2 ± 2.0 cm) versus C (54.9 ± 1.1 cm) (P = .01). Midjejunal protein content (mg/mL homogenate) was also significantly decreased in EL (38.4 ± 3.4) versus C (46.2 ± 3.7) (P = .05). Sucrase activity was not detectable in either group. Lactase activity in jejunal mucosa was not effected when expressed as units of enzyme per milliliter of homogenate (EL = 0.357 ± 0.03 v C = 0.373 ± 0.04; P = .70) and units enzyme per gram of protein (EL = 38.8 ± 4.2 v C = 34.2 ± 4.6; P = .44). We have confirmed previous studies demonstrating decreases in somatic growth, small intestinal length, and mucosal nutrient transport in rabbit fetuses following esophageal ligation. The present study demonstrates, in contrast, that the brush border disaccharidase lactase develops independently of the normal fetal swallowing mechanism with the concomitant ingestion of amniotic fluid. Implications for infants with intestinal atresias are under investigation.
AB - To investigate the effect of normal fetal swallowing and amniotic fluid ingestion on small intestinal disaccharidase development, 13 pregnant New Zealand White rabbits underwent operation on day 24 of a normal 31-day gestation. The right ovarian fetus in the bicomuate uterus underwent esophageal ligation (EL), while the contralateral left fetus underwent cervical exploration only, and served as the control (C). Rabbits were sacrificed on gestational day 31, fetal somatic measurements obtained, and the midjejunum removed for determination of disaccharidase activity and protein content. There was one maternal death, and 9 of 12 fetal pairs survived the entire study period (75%). Results are reported as mean ± SEM, analyzed by two-tailed Student's t testing with P < .05 being considered significant. Fetal weight was decreased in EL (48.6 ± 2.7 g) versus C (51.4 ± 3.2 g) (P = .06). Small intestinal length decreased in EL (49.2 ± 2.0 cm) versus C (54.9 ± 1.1 cm) (P = .01). Midjejunal protein content (mg/mL homogenate) was also significantly decreased in EL (38.4 ± 3.4) versus C (46.2 ± 3.7) (P = .05). Sucrase activity was not detectable in either group. Lactase activity in jejunal mucosa was not effected when expressed as units of enzyme per milliliter of homogenate (EL = 0.357 ± 0.03 v C = 0.373 ± 0.04; P = .70) and units enzyme per gram of protein (EL = 38.8 ± 4.2 v C = 34.2 ± 4.6; P = .44). We have confirmed previous studies demonstrating decreases in somatic growth, small intestinal length, and mucosal nutrient transport in rabbit fetuses following esophageal ligation. The present study demonstrates, in contrast, that the brush border disaccharidase lactase develops independently of the normal fetal swallowing mechanism with the concomitant ingestion of amniotic fluid. Implications for infants with intestinal atresias are under investigation.
KW - esophageal atresia
KW - Rabbit, fetal, intestinal lactase
UR - http://www.scopus.com/inward/record.url?scp=0027435293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027435293&partnerID=8YFLogxK
U2 - 10.1016/0022-3468(93)90434-M
DO - 10.1016/0022-3468(93)90434-M
M3 - Article
C2 - 8301462
AN - SCOPUS:0027435293
VL - 28
SP - 1473
EP - 1477
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
SN - 0022-3468
IS - 11
ER -