Effect of dose of GnRH analog on ovulation in mares

E. L. Squires, D. M. Moran, M. E. Farlin, D. J. Jasko, T. J. Keefe, Stuart A Meyers, E. Figueiredo, P. M. McCue, W. Jochle

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23 Scopus citations

Abstract

Proper timing of insemination for optimal conception is accomplished by frequent palpations per rectum, by ultrasonography of the preovulatory follicle and/or by treatment with hCG or GnRH. Sustained release of GnRH from implants has been shown to hasten ovulation. Therefore, 2 studies were conducted to evaluate the efficacy of a GnRH analog, deslorelin, for hastening ovulation in nonlactating cyclic mares. The GnRH implant was 2.3 × 3.7 mm and released deslorelin for 2 to 3 days. In Experiment 1, 60 nonlactating, cycling mares were assigned to 1 of 5 doses: 0, 1.2, 1.7, 2.2 and 2.7 mg per implant. Mares were assigned sequentially on the first day of estrus (Day 1). Ovaries were examined per rectum and with ultrasonography every 12 h until ovulation. Once the mares obtained a follicle >30 mm, they were injected subcutaneously with a GnRH implant. The mares were inseminated every other day during estrus with semen from 1 of 3 stallions. Pregnancy was determined with ultrasonography. Experiment 2, 40 nonlactating, cyclic mares were assigned to 1 of 5 treatments (same treatments as in Experiment 1). Data were obtained on interval to ovulation, duration of estrus and pregnancy rates at 12, 18 and 35 d after ovulation. Time to ovulation was shorter (P<0.05) in GnRH-treated mares than in control mares in the Experiment 1. Mean time to ovulation was 68, 49, 48, 47, 44 h in Experiment 1, and 91, 66, 58, 46, 58 h in Experiment 2 for mares given 0, 1.2, 1.7, 2.2 and 2.7 mg/mare in the 2 trials. Averaged for both experiments, the proportion of mares ovulating within 48 h of treatment was 40, 75, 85, 90 and 90% for 0, 1.2, 1.7, 2.2 and 2.7 mg/mare. For both experiments, there was no effect of GnRH on pregnancy rate. In summary, a subcutaneous implant containing GnRH analog induced ovulation in most mares by 48 h of injection, and there was no advantage of doses higher than 2.2 mg/mare.

Original languageEnglish (US)
Pages (from-to)757-769
Number of pages13
JournalTheriogenology
Volume41
Issue number3
DOIs
StatePublished - Feb 2 1994

Keywords

  • GnRH analog
  • horses
  • ovulation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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    Squires, E. L., Moran, D. M., Farlin, M. E., Jasko, D. J., Keefe, T. J., Meyers, S. A., Figueiredo, E., McCue, P. M., & Jochle, W. (1994). Effect of dose of GnRH analog on ovulation in mares. Theriogenology, 41(3), 757-769. https://doi.org/10.1016/0093-691X(94)90185-L