Effect of Differing Parameters on Irreversible Electroporation in a Porcine Model

Noah Canvasser, Aaron H. Lay, Ersin Koseoglu, Nicholas Kavoussi, Igor Sorokin, Jeffrey Gahan, Elena Lucas, Jeffrey A. Cadeddu

Research output: Contribution to journalArticle

Abstract

Introduction and Objective: Irreversible electroporation (IRE) is a new ablative technology to treat small renal masses. We evaluated differed ablation settings on lesion size and temperature changes in a porcine model. Materials and Methods: After Institutional Animal Care and Use Committee approval, 36 laparoscopy-guided and 16 open ablations were performed on 13 domestic female pigs. Ablation parameters studied were voltage (1000 V/cm, 1500 V/cm, or 2000 V/cm), probe exposure (1.0 or 1.5 cm), and lesion size over time (survival) (0-, 7-, or 14 day). Temperature changes were monitored during open ablations with differed settings. Gross lesion size was measured, and histologic analysis with hematoxylin and eosin and nicotinamide adenine dinucleotide staining was performed. Results: The 1000 V/cm ablations had no gross or histologic lesions. A factorial analysis of variance demonstrated that day (p = 0.56), exposure (p = 0.33), and voltage (p = 0.06) did not demonstrate statistical significance for affecting lesion size. For 1.0 cm probe exposure, 2000 V/cm did more closely approximate expected lesion size (p = 0.02) compared with 1500 V/cm. While significance was not seen for 1.5 cm probe exposure, 2000 V/cm often exceeded expected lesion volume. Only 1 of 4 temperature sensors, located adjacent to one of the IRE probes, noted a significant increase with increased voltage. However, all maximum temperatures remained less than 70°C. Conclusions: Variation in lesion volume was seen with different ablation settings in this porcine model. Maximal energy and probe exposure settings should be utilized to ensure full coverage of target volume/mass, potentially without concern for thermal injury to renal collecting system or nearby structures.

Original languageEnglish (US)
Pages (from-to)338-343
Number of pages6
JournalJournal of Endourology
Volume32
Issue number4
DOIs
StatePublished - Apr 1 2018
Externally publishedYes

Keywords

  • electroporation, kidney neoplasm, histology

ASJC Scopus subject areas

  • Urology

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    Canvasser, N., Lay, A. H., Koseoglu, E., Kavoussi, N., Sorokin, I., Gahan, J., Lucas, E., & Cadeddu, J. A. (2018). Effect of Differing Parameters on Irreversible Electroporation in a Porcine Model. Journal of Endourology, 32(4), 338-343. https://doi.org/10.1089/end.2017.0495