Effect of different linkages between chelates and monoclonal antibodies on levels of radioactivity in the liver

Shrikant V. Deshpande, Sally J. Denardo, Claude F. Meares, Michael J. Mccall, Gregory P. Adams, Gerald L Denardo

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

After injection of radiometal labeled antibodies, the radionuclide accumulates in the liver. This might be altered by a metabolizable linkage between metal chelate and antibody. Four benzyl-EDTA chelating agents were synthesized and conjugated to mouse monoclonal antibody Lym-1. Liver uptake of 111In in nontumored mice 72 h after injection was 2.2, 13.4, 7.6 and 20% for disulfide, thioether, thiourea or peptide linkages, respectively. 111In excreted in the urine was still in the benzyl-EDTA chelate form, as shown by binding to a specific anti-chelate antibody.

Original languageEnglish (US)
Pages (from-to)587-597
Number of pages11
JournalInternational Journal of Radiation Applications and Instrumentation.
Volume16
Issue number6
DOIs
StatePublished - 1989

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Radioactivity
Monoclonal Antibodies
Thiourea
Injections
Antibodies
Liver
Sulfides
Chelating Agents
Radioisotopes
Disulfides
Anti-Idiotypic Antibodies
Metals
Urine
Peptides
benzyl-EDTA
Lym-1 monoclonal antibody

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Effect of different linkages between chelates and monoclonal antibodies on levels of radioactivity in the liver. / Deshpande, Shrikant V.; Denardo, Sally J.; Meares, Claude F.; Mccall, Michael J.; Adams, Gregory P.; Denardo, Gerald L.

In: International Journal of Radiation Applications and Instrumentation., Vol. 16, No. 6, 1989, p. 587-597.

Research output: Contribution to journalArticle

Deshpande, Shrikant V. ; Denardo, Sally J. ; Meares, Claude F. ; Mccall, Michael J. ; Adams, Gregory P. ; Denardo, Gerald L. / Effect of different linkages between chelates and monoclonal antibodies on levels of radioactivity in the liver. In: International Journal of Radiation Applications and Instrumentation. 1989 ; Vol. 16, No. 6. pp. 587-597.
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