Effect of dietary linoleic acid level on lodgement, proliferation and survival of mammary tumor metastases

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High levels of dietary linoleic acid (18 : 2) have been shown to increase the spontaneous metastasis of line 4526 mouse mammary tumors. In this report, the influence of 18 : 2 on specific events of tumor metastasis, namely, lodgement, proliferation and survival, were studied using spontaneous and experimental metastasis assays with line 4526 cells. A significantly greater number of radiolabeled tumor cells lodged in the lungs of mice fed 4, 8 and 12% 18 : 2 when compared with mice fed lower levels of 18 : 2. The effect of dietary 18 : 2 appeared to be on the host tissue (lungs) and not the tumor cells. Lodgement of tumor cells first cultured in serum of mice fed 18 : 2 then injected into mice fed 1% 18 : 2 was not affected. There were no significant differences in the percentage of [3H]thymidine labeled metastatic cells from lungs of mice fed different levels of 18 : 2. However, the number of surface lung nodules that appeared in mice 21 days after injection of unlabeled line 4526 cells increased in mice fed 8 and 12% 18 : 2 compared with those fed lower levels of 18 : 2. Thus, dietary 18 : 2 may increase metastasis by influencing the lodgement, implantation and survival but not proliferation of line 4526 mouse mammary tumor cells.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalCancer Letters
Issue number2
StatePublished - 1989



  • dietary fat
  • linoleic acid
  • mammary tumors
  • metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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