Effect of dietary hempseed intake on cardiac ischemia-reperfusion injury

A. Al-Khalifa, T. G. Maddaford, M. N. Chahine, J. A. Austria, A. L. Edel, M. N. Richard, B. P. Ander, N. Gavel, M. Kopilas, R. Ganguly, P. K. Ganguly, G. N. Pierce

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Polyunsaturated fatty acids (PUFAs) have significant, cardioprotective effects against ischemia. Hempseed contains a high proportion of the PUFAs linoleic acid (LA) and α-linolenic acid (ALA), which may have opposing effects on postischemic heart performance. There are no reported data concerning the cardiovascular effects of dietary hempseed intake. A group of 40 male Sprague-Dawley rats were distributed evenly into four groups that were fed for 12 wk a normal rat chow supplemented with hempseed (5% and 10%), palm oil (1%), or a 10% partially delipidated hempseed that served as a control. Plasma ALA and γ-linolenic acid levels were significantly elevated in the rats that were fed a 5% or 10% hempseed-supplemented diet, but in heart tissue only ALA levels were significantly elevated in the rats fed these diets compared with control. After the dietary interventions were completed, postischemic heart performance was evaluated by measuring developed tension, resting tension, the rates of tension development and relaxation, and the number of extrasystoles. Hearts from rats fed a hempseed-supplemented diet exhibited significantly better postischemic recovery of maximal contractile function and enhanced rates of tension development and relaxation during reperfusion than hearts from the other groups. These hearts, however, were not protected from the occurrence of extrasystoles, nor were the increases in resting tension altered during ischemia or reperfusion as a function of any dietary intervention. Our data demonstrate that dietary hempseed can provide significant cardioprotective effects during postischemic reperfusion. This appears to be due to its highly enriched PUFA content.

Original languageEnglish (US)
Pages (from-to)R1198-R1203
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume292
Issue number3
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

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Reperfusion Injury
alpha-Linolenic Acid
Unsaturated Fatty Acids
Reperfusion
Premature Cardiac Complexes
Diet
Ischemia
Sprague Dawley Rats

Keywords

  • Alpha linolenic acid
  • Heart
  • Linoleic acid
  • Nutrition
  • Polyunsaturated fatty acid

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Al-Khalifa, A., Maddaford, T. G., Chahine, M. N., Austria, J. A., Edel, A. L., Richard, M. N., ... Pierce, G. N. (2007). Effect of dietary hempseed intake on cardiac ischemia-reperfusion injury. American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 292(3), R1198-R1203. https://doi.org/10.1152/ajpregu.00661.2006

Effect of dietary hempseed intake on cardiac ischemia-reperfusion injury. / Al-Khalifa, A.; Maddaford, T. G.; Chahine, M. N.; Austria, J. A.; Edel, A. L.; Richard, M. N.; Ander, B. P.; Gavel, N.; Kopilas, M.; Ganguly, R.; Ganguly, P. K.; Pierce, G. N.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 292, No. 3, 01.03.2007, p. R1198-R1203.

Research output: Contribution to journalArticle

Al-Khalifa, A, Maddaford, TG, Chahine, MN, Austria, JA, Edel, AL, Richard, MN, Ander, BP, Gavel, N, Kopilas, M, Ganguly, R, Ganguly, PK & Pierce, GN 2007, 'Effect of dietary hempseed intake on cardiac ischemia-reperfusion injury', American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 292, no. 3, pp. R1198-R1203. https://doi.org/10.1152/ajpregu.00661.2006
Al-Khalifa, A. ; Maddaford, T. G. ; Chahine, M. N. ; Austria, J. A. ; Edel, A. L. ; Richard, M. N. ; Ander, B. P. ; Gavel, N. ; Kopilas, M. ; Ganguly, R. ; Ganguly, P. K. ; Pierce, G. N. / Effect of dietary hempseed intake on cardiac ischemia-reperfusion injury. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2007 ; Vol. 292, No. 3. pp. R1198-R1203.
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abstract = "Polyunsaturated fatty acids (PUFAs) have significant, cardioprotective effects against ischemia. Hempseed contains a high proportion of the PUFAs linoleic acid (LA) and α-linolenic acid (ALA), which may have opposing effects on postischemic heart performance. There are no reported data concerning the cardiovascular effects of dietary hempseed intake. A group of 40 male Sprague-Dawley rats were distributed evenly into four groups that were fed for 12 wk a normal rat chow supplemented with hempseed (5{\%} and 10{\%}), palm oil (1{\%}), or a 10{\%} partially delipidated hempseed that served as a control. Plasma ALA and γ-linolenic acid levels were significantly elevated in the rats that were fed a 5{\%} or 10{\%} hempseed-supplemented diet, but in heart tissue only ALA levels were significantly elevated in the rats fed these diets compared with control. After the dietary interventions were completed, postischemic heart performance was evaluated by measuring developed tension, resting tension, the rates of tension development and relaxation, and the number of extrasystoles. Hearts from rats fed a hempseed-supplemented diet exhibited significantly better postischemic recovery of maximal contractile function and enhanced rates of tension development and relaxation during reperfusion than hearts from the other groups. These hearts, however, were not protected from the occurrence of extrasystoles, nor were the increases in resting tension altered during ischemia or reperfusion as a function of any dietary intervention. Our data demonstrate that dietary hempseed can provide significant cardioprotective effects during postischemic reperfusion. This appears to be due to its highly enriched PUFA content.",
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AU - Edel, A. L.

AU - Richard, M. N.

AU - Ander, B. P.

AU - Gavel, N.

AU - Kopilas, M.

AU - Ganguly, R.

AU - Ganguly, P. K.

AU - Pierce, G. N.

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