Abstract
We have previously shown that lymphocytic cells bind to cultured syncytiotrophoblast and that this may be important in the lymphocyte-mediated infection of trophoblast with the human immunodeficiency virus (HIV). Leukocyte-trophoblast adhesion may also have implications for normal trophoblast function. The following experiments were designed to characterize the adhesion systems that mediate the attachment of lymphocytic cells to trophoblast. Adhesion was assayed by labelling lymphocytic MOLT-4, clone 8 cells with the fluorescent marker, calcein-AM, and then incubating them with primary cultures of human syncytiotrophoblast. Adhesion was stimulated by pretreatment of the trophoblast cultures with several cytokines either alone or together. These included tumor necrosis factor-α (TNF-α), granulocyte/macrophage-colony stimulating factor (GM-CSF), interleukin-1β (IL-1β) and interferon-γ (IFN-γ). Stimulation was time- and dose-dependent. In contrast, preincubation of trophoblast cultures with anti-TNF-α antibodies for 2 days reduced MOLT adhesion by almost 50%. Preincubation with other anti-cytokine antibodies had no significant effect on adhesion. In other experiments, adhesion was measured in the presence of antibodies to known adhesion molecules. Adhesion was reduced by 50% in the presence of antibodies to α4 integrin or β1 integrin. When present together, these antibodies reduced adhesion by almost 85%. Incubation in the presence of antibodies to the very late activation antigen-4 (VLA-4; α4β1 integrin) counter-receptors, VCAM-1 and CS-1, was without effect. Adhesion was also unaffected by antibodies to LFA-1, ICAM-1, ICAM-2, LFA-2, or LFA-3. These results suggest that adhesion is mediated by an adhesion system consisting of lymphocyte VLA-4 (α4β1) and an as yet unidentified counter receptor on trophoblast.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 49-62 |
| Number of pages | 14 |
| Journal | Journal of Reproductive Immunology |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1994 |
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Keywords
- Adhesion molecules
- Cell adhesion
- Cytokines
- Integrin
- Leukocytes
- Trophoblast
ASJC Scopus subject areas
- Immunology
- Reproductive Medicine
Cite this
Effect of cytokines and anti-adhesion molecule antibodies on the adhesion of lymphocytic cells to human syncytiotrophoblast. / Douglas, Gordon C; Hu, Jinjie; Thirkill, Twanda L.; Hovanes, Karine; Sharma, Sangeeta; King, Barry F.
In: Journal of Reproductive Immunology, Vol. 27, No. 1, 1994, p. 49-62.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of cytokines and anti-adhesion molecule antibodies on the adhesion of lymphocytic cells to human syncytiotrophoblast
AU - Douglas, Gordon C
AU - Hu, Jinjie
AU - Thirkill, Twanda L.
AU - Hovanes, Karine
AU - Sharma, Sangeeta
AU - King, Barry F.
PY - 1994
Y1 - 1994
N2 - We have previously shown that lymphocytic cells bind to cultured syncytiotrophoblast and that this may be important in the lymphocyte-mediated infection of trophoblast with the human immunodeficiency virus (HIV). Leukocyte-trophoblast adhesion may also have implications for normal trophoblast function. The following experiments were designed to characterize the adhesion systems that mediate the attachment of lymphocytic cells to trophoblast. Adhesion was assayed by labelling lymphocytic MOLT-4, clone 8 cells with the fluorescent marker, calcein-AM, and then incubating them with primary cultures of human syncytiotrophoblast. Adhesion was stimulated by pretreatment of the trophoblast cultures with several cytokines either alone or together. These included tumor necrosis factor-α (TNF-α), granulocyte/macrophage-colony stimulating factor (GM-CSF), interleukin-1β (IL-1β) and interferon-γ (IFN-γ). Stimulation was time- and dose-dependent. In contrast, preincubation of trophoblast cultures with anti-TNF-α antibodies for 2 days reduced MOLT adhesion by almost 50%. Preincubation with other anti-cytokine antibodies had no significant effect on adhesion. In other experiments, adhesion was measured in the presence of antibodies to known adhesion molecules. Adhesion was reduced by 50% in the presence of antibodies to α4 integrin or β1 integrin. When present together, these antibodies reduced adhesion by almost 85%. Incubation in the presence of antibodies to the very late activation antigen-4 (VLA-4; α4β1 integrin) counter-receptors, VCAM-1 and CS-1, was without effect. Adhesion was also unaffected by antibodies to LFA-1, ICAM-1, ICAM-2, LFA-2, or LFA-3. These results suggest that adhesion is mediated by an adhesion system consisting of lymphocyte VLA-4 (α4β1) and an as yet unidentified counter receptor on trophoblast.
AB - We have previously shown that lymphocytic cells bind to cultured syncytiotrophoblast and that this may be important in the lymphocyte-mediated infection of trophoblast with the human immunodeficiency virus (HIV). Leukocyte-trophoblast adhesion may also have implications for normal trophoblast function. The following experiments were designed to characterize the adhesion systems that mediate the attachment of lymphocytic cells to trophoblast. Adhesion was assayed by labelling lymphocytic MOLT-4, clone 8 cells with the fluorescent marker, calcein-AM, and then incubating them with primary cultures of human syncytiotrophoblast. Adhesion was stimulated by pretreatment of the trophoblast cultures with several cytokines either alone or together. These included tumor necrosis factor-α (TNF-α), granulocyte/macrophage-colony stimulating factor (GM-CSF), interleukin-1β (IL-1β) and interferon-γ (IFN-γ). Stimulation was time- and dose-dependent. In contrast, preincubation of trophoblast cultures with anti-TNF-α antibodies for 2 days reduced MOLT adhesion by almost 50%. Preincubation with other anti-cytokine antibodies had no significant effect on adhesion. In other experiments, adhesion was measured in the presence of antibodies to known adhesion molecules. Adhesion was reduced by 50% in the presence of antibodies to α4 integrin or β1 integrin. When present together, these antibodies reduced adhesion by almost 85%. Incubation in the presence of antibodies to the very late activation antigen-4 (VLA-4; α4β1 integrin) counter-receptors, VCAM-1 and CS-1, was without effect. Adhesion was also unaffected by antibodies to LFA-1, ICAM-1, ICAM-2, LFA-2, or LFA-3. These results suggest that adhesion is mediated by an adhesion system consisting of lymphocyte VLA-4 (α4β1) and an as yet unidentified counter receptor on trophoblast.
KW - Adhesion molecules
KW - Cell adhesion
KW - Cytokines
KW - Integrin
KW - Leukocytes
KW - Trophoblast
UR - http://www.scopus.com/inward/record.url?scp=0027980728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027980728&partnerID=8YFLogxK
U2 - 10.1016/0165-0378(94)90014-0
DO - 10.1016/0165-0378(94)90014-0
M3 - Article
C2 - 7807471
AN - SCOPUS:0027980728
VL - 27
SP - 49
EP - 62
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
SN - 0165-0378
IS - 1
ER -