Effect of cigarette smoking on coumarin metabolism in humans

Russell E. Poland, Robert N. Pechnick, Christine C. Cloak, Yu-Jui Yvonne Wan, Inocencia Nuccio, Keh Ming Lin

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Extant data, mostly from studies in vitro, suggest that coumarin and nicotine are both metabolized by CYP2A6, a cytochrome P450 isozyme. In order to investigate this issue further, the activity of this enzyme in vivo was measured in 37 non-smokers and 37 smokers using coumarin (2.0 mg, PO) as the metabolic probe. The percentage of coumarin metabolized to 7-hydroxycoumarin in 8 h was measured in urine by high-pressure liquid chromatography. There was more than 10-fold variability in coumarin metabolism in both groups. Coumarin metabolism was significantly reduced in smokers (46.6±4.4%) as compared to non-smokers (66.4±3.5%; p≤.001). The results support previous in vitro findings that both coumarin and nicotine are metabolized, at least in part, by a common pathway, which most likely is CYP2A6.

Original languageEnglish (US)
Pages (from-to)351-354
Number of pages4
JournalNicotine and Tobacco Research
Volume2
Issue number4
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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