Effect of cigarette smoking on coumarin metabolism in humans

Russell E. Poland; Robert N. Pechnick; Christine C. Cloak; Yu-Jui Yvonne Wan; Inocencia Nuccio; Keh Ming Lin

doi:10.1080/713688151

Effect of cigarette smoking on coumarin metabolism in humans

Russell E. Poland, Robert N. Pechnick, Christine C. Cloak, Yu-Jui Yvonne Wan, Inocencia Nuccio, Keh Ming Lin

Research output: Contribution to journal › Article

14 Scopus citations

Abstract

Extant data, mostly from studies in vitro, suggest that coumarin and nicotine are both metabolized by CYP2A6, a cytochrome P450 isozyme. In order to investigate this issue further, the activity of this enzyme in vivo was measured in 37 non-smokers and 37 smokers using coumarin (2.0 mg, PO) as the metabolic probe. The percentage of coumarin metabolized to 7-hydroxycoumarin in 8 h was measured in urine by high-pressure liquid chromatography. There was more than 10-fold variability in coumarin metabolism in both groups. Coumarin metabolism was significantly reduced in smokers (46.6±4.4%) as compared to non-smokers (66.4±3.5%; p≤.001). The results support previous in vitro findings that both coumarin and nicotine are metabolized, at least in part, by a common pathway, which most likely is CYP2A6.

Original language	English (US)
Pages (from-to)	351-354
Number of pages	4
Journal	Nicotine and Tobacco Research
Volume	2
Issue number	4
DOIs	https://doi.org/10.1080/713688151
State	Published - Jan 1 2000
Externally published	Yes

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health

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Poland, R. E., Pechnick, R. N., Cloak, C. C., Wan, Y-J. Y., Nuccio, I., & Lin, K. M. (2000). Effect of cigarette smoking on coumarin metabolism in humans. Nicotine and Tobacco Research, 2(4), 351-354. https://doi.org/10.1080/713688151

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abstract = "Extant data, mostly from studies in vitro, suggest that coumarin and nicotine are both metabolized by CYP2A6, a cytochrome P450 isozyme. In order to investigate this issue further, the activity of this enzyme in vivo was measured in 37 non-smokers and 37 smokers using coumarin (2.0 mg, PO) as the metabolic probe. The percentage of coumarin metabolized to 7-hydroxycoumarin in 8 h was measured in urine by high-pressure liquid chromatography. There was more than 10-fold variability in coumarin metabolism in both groups. Coumarin metabolism was significantly reduced in smokers (46.6±4.4%) as compared to non-smokers (66.4±3.5%; p≤.001). The results support previous in vitro findings that both coumarin and nicotine are metabolized, at least in part, by a common pathway, which most likely is CYP2A6.",

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AU - Pechnick, Robert N.

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AU - Nuccio, Inocencia

AU - Lin, Keh Ming

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