Abstract
Extant data, mostly from studies in vitro, suggest that coumarin and nicotine are both metabolized by CYP2A6, a cytochrome P450 isozyme. In order to investigate this issue further, the activity of this enzyme in vivo was measured in 37 non-smokers and 37 smokers using coumarin (2.0 mg, PO) as the metabolic probe. The percentage of coumarin metabolized to 7-hydroxycoumarin in 8 h was measured in urine by high-pressure liquid chromatography. There was more than 10-fold variability in coumarin metabolism in both groups. Coumarin metabolism was significantly reduced in smokers (46.6±4.4%) as compared to non-smokers (66.4±3.5%; p≤.001). The results support previous in vitro findings that both coumarin and nicotine are metabolized, at least in part, by a common pathway, which most likely is CYP2A6.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 351-354 |
| Number of pages | 4 |
| Journal | Nicotine and Tobacco Research |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 1 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
Cite this
Effect of cigarette smoking on coumarin metabolism in humans. / Poland, Russell E.; Pechnick, Robert N.; Cloak, Christine C.; Wan, Yu-Jui Yvonne; Nuccio, Inocencia; Lin, Keh Ming.
In: Nicotine and Tobacco Research, Vol. 2, No. 4, 01.01.2000, p. 351-354.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of cigarette smoking on coumarin metabolism in humans
AU - Poland, Russell E.
AU - Pechnick, Robert N.
AU - Cloak, Christine C.
AU - Wan, Yu-Jui Yvonne
AU - Nuccio, Inocencia
AU - Lin, Keh Ming
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Extant data, mostly from studies in vitro, suggest that coumarin and nicotine are both metabolized by CYP2A6, a cytochrome P450 isozyme. In order to investigate this issue further, the activity of this enzyme in vivo was measured in 37 non-smokers and 37 smokers using coumarin (2.0 mg, PO) as the metabolic probe. The percentage of coumarin metabolized to 7-hydroxycoumarin in 8 h was measured in urine by high-pressure liquid chromatography. There was more than 10-fold variability in coumarin metabolism in both groups. Coumarin metabolism was significantly reduced in smokers (46.6±4.4%) as compared to non-smokers (66.4±3.5%; p≤.001). The results support previous in vitro findings that both coumarin and nicotine are metabolized, at least in part, by a common pathway, which most likely is CYP2A6.
AB - Extant data, mostly from studies in vitro, suggest that coumarin and nicotine are both metabolized by CYP2A6, a cytochrome P450 isozyme. In order to investigate this issue further, the activity of this enzyme in vivo was measured in 37 non-smokers and 37 smokers using coumarin (2.0 mg, PO) as the metabolic probe. The percentage of coumarin metabolized to 7-hydroxycoumarin in 8 h was measured in urine by high-pressure liquid chromatography. There was more than 10-fold variability in coumarin metabolism in both groups. Coumarin metabolism was significantly reduced in smokers (46.6±4.4%) as compared to non-smokers (66.4±3.5%; p≤.001). The results support previous in vitro findings that both coumarin and nicotine are metabolized, at least in part, by a common pathway, which most likely is CYP2A6.
UR - http://www.scopus.com/inward/record.url?scp=85047675330&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047675330&partnerID=8YFLogxK
U2 - 10.1080/713688151
DO - 10.1080/713688151
M3 - Article
C2 - 11197315
AN - SCOPUS:85047675330
VL - 2
SP - 351
EP - 354
JO - Nicotine and Tobacco Research
JF - Nicotine and Tobacco Research
SN - 1462-2203
IS - 4
ER -