Effect of chloramphenicol and the recB gene product on DNA metabolism in Escherichia coli K12 strains defective in DNA ligase

Lawrence S. Morse, Lawrence A. Beck, Crellin Pauling

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We have examined DNA strand breakage, DNA degradation, and the rate of DNA synthesis in lig and lig-recB strains of Escherichia coli K12 incubated in the presence and absence of 3 μg/ml chloramphenicol. Substantial DNA strand breakage and DNA degradation is observed in the lig strain upon growth at 40°C; however, such strand breakage and DNA degradation is not observed in the lig-recB strain. Incubation of the lig strain at 40°C in the presence of 3 μg/ml chloramphenicol reduces the amount of DNA strand breakage and DNA degradation to the level observed in the lig-recB strain. Together, these results demonstrate that exonuclease V (the recBC gene product) is responsible for the increased DNA degradation associated with DNA ligase deficiency.

Original languageEnglish (US)
Pages (from-to)79-82
Number of pages4
JournalMGG Molecular & General Genetics
Volume147
Issue number1
DOIs
StatePublished - Jan 1976

ASJC Scopus subject areas

  • Genetics

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