Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes

Nucharin Songsasen, Il Jeoung Yu, Marion S. Ratterree, Catherine A. VandeVoort, Stanley P. Leibo

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Objective: To determine the effects of chilling on the organization and distribution of tubulin and chromosomes in rhesus monkey oocytes. Design: Comparative laboratory study. Setting: Academic research laboratory. Animal(s): Eight adult female rhesus monkeys (Macaca mulatta) aged 6-16 years. Intervention(s): A total of 171 oocytes retrieved from eight rhesus monkeys were separated into nine groups. One group of control oocytes was held at 37°C during the experiment. Four groups of oocytes were rapidly cooled to 0°C and held for 1, 5, 10, or 30 minutes and then fixed and stained. Four other groups of oocytes were cooled to 0°C, held for 1, 5, 10, or 30 minutes, warmed and incubated at 37°C for 60 minutes, and then fixed and stained. Main Outcome Measure(s): Organization of cytoskeleton and chromosomes. Result(s): Exposure of rhesus oocytes to 0°C for only 1 minute resulted in complete depolymerization of tubulin. Incubation of chilled oocytes at 37°C for 60 minutes caused partial restoration of tubulin, although most oocytes exhibited abnormal alignment of chromosomes and disorganized meiotic spindles. Conclusion(s): We conclude that rhesus monkey oocytes are extremely sensitive to chilling injury. Their successful cryopreservation may require rapid cooling to outpace this injury.

Original languageEnglish (US)
Pages (from-to)818-825
Number of pages8
JournalFertility and Sterility
Volume77
Issue number4
DOIs
StatePublished - 2002

Fingerprint

Tubulin
Macaca mulatta
Oocytes
Chromosomes
Spindle Apparatus
Cryopreservation
Wounds and Injuries
Cytoskeleton
Outcome Assessment (Health Care)
Control Groups
Research

Keywords

  • Chilling injury
  • Cytoskeleton
  • Oocytes
  • Rhesus monkey

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Songsasen, N., Yu, I. J., Ratterree, M. S., VandeVoort, C. A., & Leibo, S. P. (2002). Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes. Fertility and Sterility, 77(4), 818-825. https://doi.org/10.1016/S0015-0282(01)03240-X

Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes. / Songsasen, Nucharin; Yu, Il Jeoung; Ratterree, Marion S.; VandeVoort, Catherine A.; Leibo, Stanley P.

In: Fertility and Sterility, Vol. 77, No. 4, 2002, p. 818-825.

Research output: Contribution to journalArticle

Songsasen, Nucharin ; Yu, Il Jeoung ; Ratterree, Marion S. ; VandeVoort, Catherine A. ; Leibo, Stanley P. / Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes. In: Fertility and Sterility. 2002 ; Vol. 77, No. 4. pp. 818-825.
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