Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites

Karel Smetana, Jaromír Lukáš, Věra Palečková, Jiřina Bartůňková, Fu-Tong Liu, Jiří Vacík, Hans Joachim Gabius

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The reactivity of diverse immune aspects to the presence of synthetic polymers represents one of the most important aspects of implantable device biocompatibility. In this report, we show the effect of the chemical structure of a synthetic polymer support on monocyte adhesion and selected phenotypic characteristics in vitro as a model for the initial steps of non-self-recognition of an implant. The extent of monocyte adhesion was significantly influenced by the support chemistry. The highest level of monocyte adhesion was observed on a surface copolymer of 2-hydroxyethyl methacrylate with dimethyl aminoethyl methacrylate relative to results of experiments in which poly(2-hydroxyethyl methacrylate) or the copolymer of 2-hydroxyethyl methacrylate with the sodium salt of methacrylic acid was used. Cell adhesion to the polymers tested and to glass was accompanied by enhanced expression of the carbohydrate-binding sites tested for asialoglycoprotein β-galactosides such as galectins, β-N-acetylgalactosamine, α-mannoside, specific lectin for heparin as well as the lymphokine-macrophage migration inhibitory factor in the monocytes tested. These results suggest the importance of monocyte adhesion to the biomaterial surface for their development into macrophages and further non-self-recognition of the implanted device.

Original languageEnglish (US)
Pages (from-to)1009-1014
Number of pages6
JournalBiomaterials
Volume18
Issue number14
DOIs
StatePublished - Jul 1997

Fingerprint

Galectins
Hydrogels
Binding sites
Carbohydrates
Monocytes
Adhesion
Binding Sites
Polymers
Macrophages
Asialoglycoproteins
Copolymers
Mannosides
Macrophage Migration-Inhibitory Factors
Acetylgalactosamine
Galactosides
Lymphokines
Cell adhesion
Biocompatible Materials
Equipment and Supplies
Biocompatibility

Keywords

  • Biocompatability
  • Endogenous lectins
  • Galectins
  • Macrophages

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites. / Smetana, Karel; Lukáš, Jaromír; Palečková, Věra; Bartůňková, Jiřina; Liu, Fu-Tong; Vacík, Jiří; Gabius, Hans Joachim.

In: Biomaterials, Vol. 18, No. 14, 07.1997, p. 1009-1014.

Research output: Contribution to journalArticle

Smetana, Karel ; Lukáš, Jaromír ; Palečková, Věra ; Bartůňková, Jiřina ; Liu, Fu-Tong ; Vacík, Jiří ; Gabius, Hans Joachim. / Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites. In: Biomaterials. 1997 ; Vol. 18, No. 14. pp. 1009-1014.
@article{a8a9f9fc1c1b4c3f827a0179b263cdc0,
title = "Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites",
abstract = "The reactivity of diverse immune aspects to the presence of synthetic polymers represents one of the most important aspects of implantable device biocompatibility. In this report, we show the effect of the chemical structure of a synthetic polymer support on monocyte adhesion and selected phenotypic characteristics in vitro as a model for the initial steps of non-self-recognition of an implant. The extent of monocyte adhesion was significantly influenced by the support chemistry. The highest level of monocyte adhesion was observed on a surface copolymer of 2-hydroxyethyl methacrylate with dimethyl aminoethyl methacrylate relative to results of experiments in which poly(2-hydroxyethyl methacrylate) or the copolymer of 2-hydroxyethyl methacrylate with the sodium salt of methacrylic acid was used. Cell adhesion to the polymers tested and to glass was accompanied by enhanced expression of the carbohydrate-binding sites tested for asialoglycoprotein β-galactosides such as galectins, β-N-acetylgalactosamine, α-mannoside, specific lectin for heparin as well as the lymphokine-macrophage migration inhibitory factor in the monocytes tested. These results suggest the importance of monocyte adhesion to the biomaterial surface for their development into macrophages and further non-self-recognition of the implanted device.",
keywords = "Biocompatability, Endogenous lectins, Galectins, Macrophages",
author = "Karel Smetana and Jarom{\'i}r Luk{\'a}š and Věra Palečkov{\'a} and Jiřina Bartůňkov{\'a} and Fu-Tong Liu and Jiř{\'i} Vac{\'i}k and Gabius, {Hans Joachim}",
year = "1997",
month = "7",
doi = "10.1016/S0142-9612(97)00037-9",
language = "English (US)",
volume = "18",
pages = "1009--1014",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "14",

}

TY - JOUR

T1 - Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites

AU - Smetana, Karel

AU - Lukáš, Jaromír

AU - Palečková, Věra

AU - Bartůňková, Jiřina

AU - Liu, Fu-Tong

AU - Vacík, Jiří

AU - Gabius, Hans Joachim

PY - 1997/7

Y1 - 1997/7

N2 - The reactivity of diverse immune aspects to the presence of synthetic polymers represents one of the most important aspects of implantable device biocompatibility. In this report, we show the effect of the chemical structure of a synthetic polymer support on monocyte adhesion and selected phenotypic characteristics in vitro as a model for the initial steps of non-self-recognition of an implant. The extent of monocyte adhesion was significantly influenced by the support chemistry. The highest level of monocyte adhesion was observed on a surface copolymer of 2-hydroxyethyl methacrylate with dimethyl aminoethyl methacrylate relative to results of experiments in which poly(2-hydroxyethyl methacrylate) or the copolymer of 2-hydroxyethyl methacrylate with the sodium salt of methacrylic acid was used. Cell adhesion to the polymers tested and to glass was accompanied by enhanced expression of the carbohydrate-binding sites tested for asialoglycoprotein β-galactosides such as galectins, β-N-acetylgalactosamine, α-mannoside, specific lectin for heparin as well as the lymphokine-macrophage migration inhibitory factor in the monocytes tested. These results suggest the importance of monocyte adhesion to the biomaterial surface for their development into macrophages and further non-self-recognition of the implanted device.

AB - The reactivity of diverse immune aspects to the presence of synthetic polymers represents one of the most important aspects of implantable device biocompatibility. In this report, we show the effect of the chemical structure of a synthetic polymer support on monocyte adhesion and selected phenotypic characteristics in vitro as a model for the initial steps of non-self-recognition of an implant. The extent of monocyte adhesion was significantly influenced by the support chemistry. The highest level of monocyte adhesion was observed on a surface copolymer of 2-hydroxyethyl methacrylate with dimethyl aminoethyl methacrylate relative to results of experiments in which poly(2-hydroxyethyl methacrylate) or the copolymer of 2-hydroxyethyl methacrylate with the sodium salt of methacrylic acid was used. Cell adhesion to the polymers tested and to glass was accompanied by enhanced expression of the carbohydrate-binding sites tested for asialoglycoprotein β-galactosides such as galectins, β-N-acetylgalactosamine, α-mannoside, specific lectin for heparin as well as the lymphokine-macrophage migration inhibitory factor in the monocytes tested. These results suggest the importance of monocyte adhesion to the biomaterial surface for their development into macrophages and further non-self-recognition of the implanted device.

KW - Biocompatability

KW - Endogenous lectins

KW - Galectins

KW - Macrophages

UR - http://www.scopus.com/inward/record.url?scp=0342872066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0342872066&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(97)00037-9

DO - 10.1016/S0142-9612(97)00037-9

M3 - Article

VL - 18

SP - 1009

EP - 1014

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 14

ER -