Effect of cell shrinkage on permeability of cultured bovine aortic endothelia and frog mesenteric capillaries

M. Kajimura, Martha E O'Donnell, F. E. Curry

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

1. We have tested the hypothesis that a reduction in endothelial cell volume increases microvessel permeability and that the degree of endothelial cell attachment to their basement membranes determines the magnitude of permeability changes caused by a reduction in endothelial cell volume. 2. A decrease in endothelial cell volume was imposed on both intact microvessels and cultured endothelial monolayers by raising osmolarity by 100 mosmol l-1. 3. We found that hypertonic solutions did not increase the hydraulic permeability (L(p)) of individually perfused venular microvessels in frog mesentery when the perfusate contained albumin. Hypertonic solutions did increase L(p), however, after we perfused the microvessels with the peptide Gly-Arg-Gly-Asp-Thr-Pro (GRGDTP; 0.3 mmol l-1), to disrupt integrin-dependent endothelial cell (EC) attachment to the extracellular matrix (ECM). 4. After albumin was removed from the perfusate, hypertonic solutions increased L(p) of microvessels and the permeability of endothelial monolayers to α-lactalbumin. 5. Our findings indicate that endothelial cell integrin-ECM binding plays a role in transducing changes in cell volume and/or shape into changes in permeability. We hypothesize that removal of albumin from the vascular perfusate may compromise EC-ECM interactions via an integrin-dependent mechanism.

Original languageEnglish (US)
Pages (from-to)413-425
Number of pages13
JournalJournal of Physiology
Volume503
Issue number2
DOIs
StatePublished - Sep 1 1997

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Anura
Endothelium
Permeability
Endothelial Cells
Microvessels
fibronectin attachment peptide
Hypertonic Solutions
Cell Size
Integrins
Extracellular Matrix
Albumins
Lactalbumin
Mesentery
Cell Shape
Basement Membrane
Osmolar Concentration
Blood Vessels
Peptides

ASJC Scopus subject areas

  • Physiology

Cite this

Effect of cell shrinkage on permeability of cultured bovine aortic endothelia and frog mesenteric capillaries. / Kajimura, M.; O'Donnell, Martha E; Curry, F. E.

In: Journal of Physiology, Vol. 503, No. 2, 01.09.1997, p. 413-425.

Research output: Contribution to journalArticle

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