Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger

C. Morisseau, G. Venturi, P. Moussou, J. Baratti

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The highly enantioselective epoxide hydrolase from Aspergillus niger is well utilized as biocatalysts for the preparation of enantiopure chiral epoxides and diols. Both growth of the fungus and EH activity production were found greatly affected by changing the carbon or the nitrogen source with fructose and corn steep liquor being the best. Their concentrations were optimized (10 g.l-1 of fructose and 15 g.l-1 of corn steep) which resulted in an increase of both the biomass produced (31%) and the epoxide hydrolase specific activity (38%). The results obtained suggested a complex regulation of the EH production. On the whole, a two times increase of the total EH activity was obtained.

Original languageEnglish (US)
Pages (from-to)805-809
Number of pages5
JournalBiotechnology Techniques
Volume12
Issue number11
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Epoxide Hydrolases
Hydrolases
Fructose
Aspergillus niger
Aspergillus
Zea mays
Nitrogen
Carbon
Biocatalysts
Epoxy Compounds
Fungi
Biomass
Enzymes
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger. / Morisseau, C.; Venturi, G.; Moussou, P.; Baratti, J.

In: Biotechnology Techniques, Vol. 12, No. 11, 1998, p. 805-809.

Research output: Contribution to journalArticle

Morisseau, C. ; Venturi, G. ; Moussou, P. ; Baratti, J. / Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger. In: Biotechnology Techniques. 1998 ; Vol. 12, No. 11. pp. 805-809.
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