Abstract
The highly enantioselective epoxide hydrolase from Aspergillus niger is well utilized as biocatalysts for the preparation of enantiopure chiral epoxides and diols. Both growth of the fungus and EH activity production were found greatly affected by changing the carbon or the nitrogen source with fructose and corn steep liquor being the best. Their concentrations were optimized (10 g.l-1 of fructose and 15 g.l-1 of corn steep) which resulted in an increase of both the biomass produced (31%) and the epoxide hydrolase specific activity (38%). The results obtained suggested a complex regulation of the EH production. On the whole, a two times increase of the total EH activity was obtained.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 805-809 |
| Number of pages | 5 |
| Journal | Biotechnology Techniques |
| Volume | 12 |
| Issue number | 11 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biochemistry
Cite this
Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger. / Morisseau, C.; Venturi, G.; Moussou, P.; Baratti, J.
In: Biotechnology Techniques, Vol. 12, No. 11, 1998, p. 805-809.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger
AU - Morisseau, C.
AU - Venturi, G.
AU - Moussou, P.
AU - Baratti, J.
PY - 1998
Y1 - 1998
N2 - The highly enantioselective epoxide hydrolase from Aspergillus niger is well utilized as biocatalysts for the preparation of enantiopure chiral epoxides and diols. Both growth of the fungus and EH activity production were found greatly affected by changing the carbon or the nitrogen source with fructose and corn steep liquor being the best. Their concentrations were optimized (10 g.l-1 of fructose and 15 g.l-1 of corn steep) which resulted in an increase of both the biomass produced (31%) and the epoxide hydrolase specific activity (38%). The results obtained suggested a complex regulation of the EH production. On the whole, a two times increase of the total EH activity was obtained.
AB - The highly enantioselective epoxide hydrolase from Aspergillus niger is well utilized as biocatalysts for the preparation of enantiopure chiral epoxides and diols. Both growth of the fungus and EH activity production were found greatly affected by changing the carbon or the nitrogen source with fructose and corn steep liquor being the best. Their concentrations were optimized (10 g.l-1 of fructose and 15 g.l-1 of corn steep) which resulted in an increase of both the biomass produced (31%) and the epoxide hydrolase specific activity (38%). The results obtained suggested a complex regulation of the EH production. On the whole, a two times increase of the total EH activity was obtained.
UR - http://www.scopus.com/inward/record.url?scp=0032435710&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032435710&partnerID=8YFLogxK
U2 - 10.1023/A:1008891929478
DO - 10.1023/A:1008891929478
M3 - Article
AN - SCOPUS:0032435710
VL - 12
SP - 805
EP - 809
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 11
ER -