Effect of C-reactive protein on chemokine expression in human aortic endothelial cells

Sridevi Devaraj, Pappanaicken R. Kumaresan, Ishwarlal Jialal

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRP. Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31%, P < 0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity.

Original languageEnglish (US)
Pages (from-to)405-410
Number of pages6
JournalJournal of Molecular and Cellular Cardiology
Volume36
Issue number3
DOIs
StatePublished - Mar 2004

Fingerprint

Chemokines
C-Reactive Protein
Endothelial Cells
Interleukin-8
NF-kappa B
Cell Adhesion
Monocytes
Atherosclerosis
Up-Regulation
CXC Chemokines
Blocking Antibodies
Chemokine CCL2
Human Umbilical Vein Endothelial Cells
Atherosclerotic Plaques
Human Activities
Cardiovascular Diseases
Observation
Inflammation
Messenger RNA
Proteins

Keywords

  • C-reactive protein
  • Chemokine
  • Endothelium
  • Inflammation
  • Interleukin

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Effect of C-reactive protein on chemokine expression in human aortic endothelial cells. / Devaraj, Sridevi; Kumaresan, Pappanaicken R.; Jialal, Ishwarlal.

In: Journal of Molecular and Cellular Cardiology, Vol. 36, No. 3, 03.2004, p. 405-410.

Research output: Contribution to journalArticle

Devaraj, Sridevi ; Kumaresan, Pappanaicken R. ; Jialal, Ishwarlal. / Effect of C-reactive protein on chemokine expression in human aortic endothelial cells. In: Journal of Molecular and Cellular Cardiology. 2004 ; Vol. 36, No. 3. pp. 405-410.
@article{a3fa70f19ea14cf690f587afc520c7ab,
title = "Effect of C-reactive protein on chemokine expression in human aortic endothelial cells",
abstract = "Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRP. Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31{\%}, P < 0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity.",
keywords = "C-reactive protein, Chemokine, Endothelium, Inflammation, Interleukin",
author = "Sridevi Devaraj and Kumaresan, {Pappanaicken R.} and Ishwarlal Jialal",
year = "2004",
month = "3",
doi = "10.1016/j.yjmcc.2003.12.005",
language = "English (US)",
volume = "36",
pages = "405--410",
journal = "Journal of Molecular and Cellular Cardiology",
issn = "0022-2828",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Effect of C-reactive protein on chemokine expression in human aortic endothelial cells

AU - Devaraj, Sridevi

AU - Kumaresan, Pappanaicken R.

AU - Jialal, Ishwarlal

PY - 2004/3

Y1 - 2004/3

N2 - Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRP. Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31%, P < 0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity.

AB - Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRP. Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31%, P < 0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity.

KW - C-reactive protein

KW - Chemokine

KW - Endothelium

KW - Inflammation

KW - Interleukin

UR - http://www.scopus.com/inward/record.url?scp=1542328869&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542328869&partnerID=8YFLogxK

U2 - 10.1016/j.yjmcc.2003.12.005

DO - 10.1016/j.yjmcc.2003.12.005

M3 - Article

VL - 36

SP - 405

EP - 410

JO - Journal of Molecular and Cellular Cardiology

JF - Journal of Molecular and Cellular Cardiology

SN - 0022-2828

IS - 3

ER -