Effect of C-fiber-mediated, ozone-induced rapid shallow breathing on airway epithelial injury in rats

Edward S Schelegle, M. F. Alfaro, L. Putney, M. Stovall, N. Tyler, D. M. Hyde

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We examined the relationship between C-fiber-mediated, ozone-induced rapid shallow breathing and airway epithelial cell injury at different airway sites within the lower respiratory tract of conscious Wistar rats (n = 24). We combined an acute 8-h ozone inhalation with vagal perineural capsaicin treatment, a selective C-fiber conduction block, and 5-bromo-2′-deoxyuridine (BrdU) labeling as an index of epithelial injury. Vehicle-treated rats that inhaled ozone developed a rapid shallow breathing pattern during ozone inhalation, whereas the capsaicin-treated rats that inhaled ozone showed no changes in respiratory frequency. In vehicle-treated, ozone-exposed rats that developed rapid shallow breathing, a progressive increase in BrdU-labeling density (no. of BrdU-labeled cells/mm2 airway) was observed starting at the bifurcation of the left main stem bronchi (central airway) and going down either a short or long airway path. In vehicle-treated, ozone-exposed rats, terminal bronchioles supplied by short and long airway paths had a similar degree of BrdU-labeling density that was significantly (P < 0.05) greater than the BrdU-labeling density of the proximal airways that supply them. In contrast, the attenuation of rapid shallow breathing produced by capsaicin treatment resulted in a significantly reduced BrdU-labeling density in the terminal bronchioles supplied by short airway paths compared with the terminal bronchioles supplied by long airway paths. Our data indicate that ozone-induced rapid shallow breathing protects large conducting airways while producing a more even distribution of injury to terminal bronchioles.

Original languageEnglish (US)
Pages (from-to)1611-1618
Number of pages8
JournalJournal of Applied Physiology
Volume91
Issue number4
StatePublished - 2001

Fingerprint

Unmyelinated Nerve Fibers
Ozone
Respiration
Deoxyuridine
Bronchioles
Wounds and Injuries
Capsaicin
Inhalation
Bromodeoxyuridine
Bronchi
Respiratory System
Wistar Rats
Epithelial Cells

Keywords

  • 5-bromo-2′-deoxyuridine
  • Capsaicin
  • Terminal bronchioles

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effect of C-fiber-mediated, ozone-induced rapid shallow breathing on airway epithelial injury in rats. / Schelegle, Edward S; Alfaro, M. F.; Putney, L.; Stovall, M.; Tyler, N.; Hyde, D. M.

In: Journal of Applied Physiology, Vol. 91, No. 4, 2001, p. 1611-1618.

Research output: Contribution to journalArticle

Schelegle, ES, Alfaro, MF, Putney, L, Stovall, M, Tyler, N & Hyde, DM 2001, 'Effect of C-fiber-mediated, ozone-induced rapid shallow breathing on airway epithelial injury in rats', Journal of Applied Physiology, vol. 91, no. 4, pp. 1611-1618.
Schelegle, Edward S ; Alfaro, M. F. ; Putney, L. ; Stovall, M. ; Tyler, N. ; Hyde, D. M. / Effect of C-fiber-mediated, ozone-induced rapid shallow breathing on airway epithelial injury in rats. In: Journal of Applied Physiology. 2001 ; Vol. 91, No. 4. pp. 1611-1618.
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