Effect of age on the frequency, cell cycle, and lineage maturation of rhesus monkey (Macaca mulatta) CD34+ and hematopoietic progenitor cells

Charles C Lee, Misty D. Fletcher, Alice F Tarantal

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23 Citations (Scopus)

Abstract

The effects of maturation and aging on hematopoietic progenitor cells, blood and bone marrow from second- and third-trimester fetal, newborn, infant, adult, and aged rhesus monkeys (Macaca mulatta) were analyzed. CD34+ cells were immunoselected and stained with propidium iodide for cell cycle analysis. Blood and bone marrow mononuclear cells were plated in methylcellulose, and erythroid and myeloid progenitors were grown and counted. A higher frequency of circulating CD34+CD38- and CD34 +DR- cells was observed in second-trimester fetuses compared with the other age groups. The frequency of bone marrow CD34 +CD38- and CD34+DR- cells declined in adult and aged animals when compared with the younger age groups. Cell-cycle analysis showed 4.5% second-trimester fetal bone marrow CD34+ cells entering the G2/M phase, compared with 1.7% CD34+ cells in aged animals. More than 95% of circulating CD34+ cells remained quiescent for most age groups, except for second-trimester fetuses. Adult marrow myeloid progenitors were found in a lower quantity when compared with third-trimester fetuses, whereas erythroid progenitors were greatest in early-gestation fetuses and adults. The results of these studies suggest that 1) the greatest quantity of CD34+CD38- and CD34 +DR- cells was found in fetal and infant bone marrow, 2) the frequency of cycling CD34+ cells declines with maturation and aging, and 3) an age-dependent difference in lineage commitment occurs.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalPediatric Research
Volume58
Issue number2
DOIs
StatePublished - Aug 2005

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Cell Lineage
Hematopoietic Stem Cells
Macaca mulatta
Cell Cycle
Second Pregnancy Trimester
Fetus
Bone Marrow
Age Groups
Third Pregnancy Trimester
Bone Marrow Cells
Methylcellulose
Propidium
G2 Phase
Cell Division
Newborn Infant
Pregnancy

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

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title = "Effect of age on the frequency, cell cycle, and lineage maturation of rhesus monkey (Macaca mulatta) CD34+ and hematopoietic progenitor cells",
abstract = "The effects of maturation and aging on hematopoietic progenitor cells, blood and bone marrow from second- and third-trimester fetal, newborn, infant, adult, and aged rhesus monkeys (Macaca mulatta) were analyzed. CD34+ cells were immunoselected and stained with propidium iodide for cell cycle analysis. Blood and bone marrow mononuclear cells were plated in methylcellulose, and erythroid and myeloid progenitors were grown and counted. A higher frequency of circulating CD34+CD38- and CD34 +DR- cells was observed in second-trimester fetuses compared with the other age groups. The frequency of bone marrow CD34 +CD38- and CD34+DR- cells declined in adult and aged animals when compared with the younger age groups. Cell-cycle analysis showed 4.5{\%} second-trimester fetal bone marrow CD34+ cells entering the G2/M phase, compared with 1.7{\%} CD34+ cells in aged animals. More than 95{\%} of circulating CD34+ cells remained quiescent for most age groups, except for second-trimester fetuses. Adult marrow myeloid progenitors were found in a lower quantity when compared with third-trimester fetuses, whereas erythroid progenitors were greatest in early-gestation fetuses and adults. The results of these studies suggest that 1) the greatest quantity of CD34+CD38- and CD34 +DR- cells was found in fetal and infant bone marrow, 2) the frequency of cycling CD34+ cells declines with maturation and aging, and 3) an age-dependent difference in lineage commitment occurs.",
author = "Lee, {Charles C} and Fletcher, {Misty D.} and Tarantal, {Alice F}",
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T1 - Effect of age on the frequency, cell cycle, and lineage maturation of rhesus monkey (Macaca mulatta) CD34+ and hematopoietic progenitor cells

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AU - Fletcher, Misty D.

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N2 - The effects of maturation and aging on hematopoietic progenitor cells, blood and bone marrow from second- and third-trimester fetal, newborn, infant, adult, and aged rhesus monkeys (Macaca mulatta) were analyzed. CD34+ cells were immunoselected and stained with propidium iodide for cell cycle analysis. Blood and bone marrow mononuclear cells were plated in methylcellulose, and erythroid and myeloid progenitors were grown and counted. A higher frequency of circulating CD34+CD38- and CD34 +DR- cells was observed in second-trimester fetuses compared with the other age groups. The frequency of bone marrow CD34 +CD38- and CD34+DR- cells declined in adult and aged animals when compared with the younger age groups. Cell-cycle analysis showed 4.5% second-trimester fetal bone marrow CD34+ cells entering the G2/M phase, compared with 1.7% CD34+ cells in aged animals. More than 95% of circulating CD34+ cells remained quiescent for most age groups, except for second-trimester fetuses. Adult marrow myeloid progenitors were found in a lower quantity when compared with third-trimester fetuses, whereas erythroid progenitors were greatest in early-gestation fetuses and adults. The results of these studies suggest that 1) the greatest quantity of CD34+CD38- and CD34 +DR- cells was found in fetal and infant bone marrow, 2) the frequency of cycling CD34+ cells declines with maturation and aging, and 3) an age-dependent difference in lineage commitment occurs.

AB - The effects of maturation and aging on hematopoietic progenitor cells, blood and bone marrow from second- and third-trimester fetal, newborn, infant, adult, and aged rhesus monkeys (Macaca mulatta) were analyzed. CD34+ cells were immunoselected and stained with propidium iodide for cell cycle analysis. Blood and bone marrow mononuclear cells were plated in methylcellulose, and erythroid and myeloid progenitors were grown and counted. A higher frequency of circulating CD34+CD38- and CD34 +DR- cells was observed in second-trimester fetuses compared with the other age groups. The frequency of bone marrow CD34 +CD38- and CD34+DR- cells declined in adult and aged animals when compared with the younger age groups. Cell-cycle analysis showed 4.5% second-trimester fetal bone marrow CD34+ cells entering the G2/M phase, compared with 1.7% CD34+ cells in aged animals. More than 95% of circulating CD34+ cells remained quiescent for most age groups, except for second-trimester fetuses. Adult marrow myeloid progenitors were found in a lower quantity when compared with third-trimester fetuses, whereas erythroid progenitors were greatest in early-gestation fetuses and adults. The results of these studies suggest that 1) the greatest quantity of CD34+CD38- and CD34 +DR- cells was found in fetal and infant bone marrow, 2) the frequency of cycling CD34+ cells declines with maturation and aging, and 3) an age-dependent difference in lineage commitment occurs.

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