Effect of age on in vivo rates of mitochondrial protein synthesis in human skeletal muscle

Olav E. Rooyackers, Deborah B. Adey, Philip A. Ades, K. Sreekumaran Nair

Research output: Contribution to journalArticle

413 Citations (Scopus)

Abstract

A progressive decline in muscle performance in the rapidly expanding aging population is causing a dramatic increase in disability and health care costs. A decrease in muscle endurance capacity due to mitochondrial decay likely contributes to this decline in muscle performance. We developed a novel stable isotope technique to measure in vivo rates of mitochondrial protein synthesis in human skeletal muscle using needle biopsy samples and applied this technique to elucidate a potential mechanism for the age- related decline in the mitochondrial content and function of skeletal muscle. The fractional rate of muscle mitochondrial protein synthesis in young humans (24 ± 1 year) was 0.081 ± 0.004% · h-1, and this rate declined to 0.047 ± 0.005% · h-1 by middle age (54 ± 1 year; P < 0.01). No further decline in the rate of mitochondrial protein synthesis (0.051 ± 0.004% · h-1) occurred with advancing age (73 ± 2 years). The mitochondrial synthesis rate was about 95% higher than that of mixed protein in the young, whereas it was approximately 35% higher in the middle-aged and elderly subjects. In addition, decreasing activities of mitochondrial enzymes were observed in muscle homogenates (cytochrome c oxidase and citrate synthase) and in isolated mitochondria (citrate synthase) with increasing age, indicating declines in muscle oxidative capacity and mitochondrial function, respectively. The decrease in the rates of mitochondrial protein synthesis is likely to be responsible for this decline in muscle oxidative capacity and mitochondrial function. These changes in muscle mitochondrial protein metabolism may contribute to the age-related decline in aerobic capacity and muscle performance.

Original languageEnglish (US)
Pages (from-to)15364-15369
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number26
DOIs
StatePublished - Dec 24 1996
Externally publishedYes

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Mitochondrial Proteins
Skeletal Muscle
Muscles
Citrate (si)-Synthase
Muscle Proteins
Needle Biopsy
Electron Transport Complex IV
Isotopes
Health Care Costs
Mitochondria
Enzymes
Population
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Effect of age on in vivo rates of mitochondrial protein synthesis in human skeletal muscle. / Rooyackers, Olav E.; Adey, Deborah B.; Ades, Philip A.; Nair, K. Sreekumaran.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 26, 24.12.1996, p. 15364-15369.

Research output: Contribution to journalArticle

Rooyackers, Olav E. ; Adey, Deborah B. ; Ades, Philip A. ; Nair, K. Sreekumaran. / Effect of age on in vivo rates of mitochondrial protein synthesis in human skeletal muscle. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 26. pp. 15364-15369.
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