Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb

K. C. Eiffert, J. S. Stern, Barbara A Horwitz, L. M. Larkin, R. B. McDonald

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Abstract

The purpose of this investigation was to evaluate insulin-stimulated skeletal muscle glucose uptake in male Fischer 344 rats, age 6, 12, and 27 mo, fed either a sucrose (S: 66.6% sucrose, 17.6% protein, 6.4% fat) or sucrose-free (SF: 66.6% starch, 17.6% protein, 6.4% fat) diet for 3 mo. Skeletal muscle glucose uptake (R(g)) in perfused hindlimbs was estimated from the uptake and subsequent phosphorylation of radiolabeled 2-deoxyglucose (2DG) in the gastrocnemius (GN), extensor digitorum longus (EDL), and soleus (SOL) muscles. Rat hindlimbs were perfused at a rate of 10 ml · min-1 with a modified Krebs Henseleit buffer containing bovine red blood cells (hematocrit: 40%) and 5.85 mmole · L-1 glucose along with 358 pmoles · L-1 followed by 3580 pmoles · L-1 insulin. There was no effect of diet on plasma glucose levels measured at weeks 1, 7, and 11 of the dietary period. A significant effect of age on estimated glucose uptake in the GN was demonstrated due primarily to greater uptake in the 27-mo compared to the 6- mo-old animals. This significant effect of age was not evident in the EDL or SOL, nor were there significant effects of diet in any muscle. These data suggest that insulin-stimulated glucose uptake in perfused hindlimbs is not attenuated with senescence or with the feeding of a sucrose diet for 3 months.

Original languageEnglish (US)
JournalJournals of Gerontology
Volume48
Issue number6
StatePublished - 1993

ASJC Scopus subject areas

  • Aging

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    Eiffert, K. C., Stern, J. S., Horwitz, B. A., Larkin, L. M., & McDonald, R. B. (1993). Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb. Journals of Gerontology, 48(6).