Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb

K. C. Eiffert, J. S. Stern, Barbara A Horwitz, L. M. Larkin, R. B. McDonald

Research output: Contribution to journalArticle

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Abstract

The purpose of this investigation was to evaluate insulin-stimulated skeletal muscle glucose uptake in male Fischer 344 rats, age 6, 12, and 27 mo, fed either a sucrose (S: 66.6% sucrose, 17.6% protein, 6.4% fat) or sucrose-free (SF: 66.6% starch, 17.6% protein, 6.4% fat) diet for 3 mo. Skeletal muscle glucose uptake (R(g)) in perfused hindlimbs was estimated from the uptake and subsequent phosphorylation of radiolabeled 2-deoxyglucose (2DG) in the gastrocnemius (GN), extensor digitorum longus (EDL), and soleus (SOL) muscles. Rat hindlimbs were perfused at a rate of 10 ml · min-1 with a modified Krebs Henseleit buffer containing bovine red blood cells (hematocrit: 40%) and 5.85 mmole · L-1 glucose along with 358 pmoles · L-1 followed by 3580 pmoles · L-1 insulin. There was no effect of diet on plasma glucose levels measured at weeks 1, 7, and 11 of the dietary period. A significant effect of age on estimated glucose uptake in the GN was demonstrated due primarily to greater uptake in the 27-mo compared to the 6- mo-old animals. This significant effect of age was not evident in the EDL or SOL, nor were there significant effects of diet in any muscle. These data suggest that insulin-stimulated glucose uptake in perfused hindlimbs is not attenuated with senescence or with the feeding of a sucrose diet for 3 months.

Original languageEnglish (US)
JournalJournals of Gerontology
Volume48
Issue number6
StatePublished - 1993

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Deoxyglucose
Hindlimb
Sucrose
Diet
Glucose
Skeletal Muscle
Insulin
Fats
Inbred F344 Rats
Hematocrit
Starch
Proteins
Erythrocytes
Phosphorylation
Muscles

ASJC Scopus subject areas

  • Aging

Cite this

Eiffert, K. C., Stern, J. S., Horwitz, B. A., Larkin, L. M., & McDonald, R. B. (1993). Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb. Journals of Gerontology, 48(6).

Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb. / Eiffert, K. C.; Stern, J. S.; Horwitz, Barbara A; Larkin, L. M.; McDonald, R. B.

In: Journals of Gerontology, Vol. 48, No. 6, 1993.

Research output: Contribution to journalArticle

Eiffert, KC, Stern, JS, Horwitz, BA, Larkin, LM & McDonald, RB 1993, 'Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb', Journals of Gerontology, vol. 48, no. 6.
Eiffert KC, Stern JS, Horwitz BA, Larkin LM, McDonald RB. Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb. Journals of Gerontology. 1993;48(6).
Eiffert, K. C. ; Stern, J. S. ; Horwitz, Barbara A ; Larkin, L. M. ; McDonald, R. B. / Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb. In: Journals of Gerontology. 1993 ; Vol. 48, No. 6.
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