Effect of age and castration on serum anti-Müllerian hormone concentration in male alpacas

Michela Ciccarelli, Ahmed Tibary, Alexis J. Campbell, Alan J Conley

Research output: Contribution to journalArticle

Abstract

The synthesis of anti-Müllerian Hormone (AMH) by the Sertoli cells in males is crucial for sexual differentiation. There are no studies on AMH in Camelids. The objectives of this research were to 1) compare AMH serum concentrations in prepubertal and adult male alpacas and 2) determine the effect of castration on these concentrations in adult males to provide a validation of a commercial AMH test in alpacas. Serum samples were obtained from 15 prepubertal animals (5 for each age groups of 6, 7 and 8 months) and from 5 adult males (age 5–9 years), pre- and 24 h post-castration. AMH was determined with a quantitative ELISA according to the manufacture's instructions. There was not significant difference (P < 0.05) in AMH level (pg/ml) between pre-pubertal (549.9 ± 120.8, 789.4 ± 172.3, 597.5 ± 177.3 for ages 4, 7, and 8 months, respectively) and adult alpacas (938.7 ± 175.9). In adult males, AMH concentration decreased significantly following castration (P < 0.05) (938.7 ± 383.5 pg/ml) pre-castration, and 222.1 ± 116.5 pg/ml) after castration). There was a positive correlation between testosterone levels and AMH. In conclusion, the quantitative assay used is a reliable test to determine AMH in alpacas. The AMH level in prepubertal and adult alpacas appear to not differ, contrarily from other mammals, this requires further investigation. The decrease in serum AMH concentrations after castration suggests that measurement of this hormone can be used to diagnose bilateral cryptorchid or hemicastrated unilateral cryptorchid animals in this species.

Original languageEnglish (US)
Pages (from-to)174-177
Number of pages4
JournalTheriogenology
Volume105
DOIs
StatePublished - Jan 1 2018

Fingerprint

New World Camelids
alpacas
Castration
castration
hormones
Hormones
Serum
Sex Differentiation
Camelidae
Sertoli Cells
Sertoli cells
sexual development
testosterone
Testosterone
Mammals
animals
manufacturing

Keywords

  • AMH
  • Camelids
  • Cryptorchid

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Effect of age and castration on serum anti-Müllerian hormone concentration in male alpacas. / Ciccarelli, Michela; Tibary, Ahmed; Campbell, Alexis J.; Conley, Alan J.

In: Theriogenology, Vol. 105, 01.01.2018, p. 174-177.

Research output: Contribution to journalArticle

Ciccarelli, Michela ; Tibary, Ahmed ; Campbell, Alexis J. ; Conley, Alan J. / Effect of age and castration on serum anti-Müllerian hormone concentration in male alpacas. In: Theriogenology. 2018 ; Vol. 105. pp. 174-177.
@article{c756bb0ec38b4858a6f2991d4ddc9c07,
title = "Effect of age and castration on serum anti-M{\"u}llerian hormone concentration in male alpacas",
abstract = "The synthesis of anti-M{\"u}llerian Hormone (AMH) by the Sertoli cells in males is crucial for sexual differentiation. There are no studies on AMH in Camelids. The objectives of this research were to 1) compare AMH serum concentrations in prepubertal and adult male alpacas and 2) determine the effect of castration on these concentrations in adult males to provide a validation of a commercial AMH test in alpacas. Serum samples were obtained from 15 prepubertal animals (5 for each age groups of 6, 7 and 8 months) and from 5 adult males (age 5–9 years), pre- and 24 h post-castration. AMH was determined with a quantitative ELISA according to the manufacture's instructions. There was not significant difference (P < 0.05) in AMH level (pg/ml) between pre-pubertal (549.9 ± 120.8, 789.4 ± 172.3, 597.5 ± 177.3 for ages 4, 7, and 8 months, respectively) and adult alpacas (938.7 ± 175.9). In adult males, AMH concentration decreased significantly following castration (P < 0.05) (938.7 ± 383.5 pg/ml) pre-castration, and 222.1 ± 116.5 pg/ml) after castration). There was a positive correlation between testosterone levels and AMH. In conclusion, the quantitative assay used is a reliable test to determine AMH in alpacas. The AMH level in prepubertal and adult alpacas appear to not differ, contrarily from other mammals, this requires further investigation. The decrease in serum AMH concentrations after castration suggests that measurement of this hormone can be used to diagnose bilateral cryptorchid or hemicastrated unilateral cryptorchid animals in this species.",
keywords = "AMH, Camelids, Cryptorchid",
author = "Michela Ciccarelli and Ahmed Tibary and Campbell, {Alexis J.} and Conley, {Alan J}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.theriogenology.2017.09.032",
language = "English (US)",
volume = "105",
pages = "174--177",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Effect of age and castration on serum anti-Müllerian hormone concentration in male alpacas

AU - Ciccarelli, Michela

AU - Tibary, Ahmed

AU - Campbell, Alexis J.

AU - Conley, Alan J

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The synthesis of anti-Müllerian Hormone (AMH) by the Sertoli cells in males is crucial for sexual differentiation. There are no studies on AMH in Camelids. The objectives of this research were to 1) compare AMH serum concentrations in prepubertal and adult male alpacas and 2) determine the effect of castration on these concentrations in adult males to provide a validation of a commercial AMH test in alpacas. Serum samples were obtained from 15 prepubertal animals (5 for each age groups of 6, 7 and 8 months) and from 5 adult males (age 5–9 years), pre- and 24 h post-castration. AMH was determined with a quantitative ELISA according to the manufacture's instructions. There was not significant difference (P < 0.05) in AMH level (pg/ml) between pre-pubertal (549.9 ± 120.8, 789.4 ± 172.3, 597.5 ± 177.3 for ages 4, 7, and 8 months, respectively) and adult alpacas (938.7 ± 175.9). In adult males, AMH concentration decreased significantly following castration (P < 0.05) (938.7 ± 383.5 pg/ml) pre-castration, and 222.1 ± 116.5 pg/ml) after castration). There was a positive correlation between testosterone levels and AMH. In conclusion, the quantitative assay used is a reliable test to determine AMH in alpacas. The AMH level in prepubertal and adult alpacas appear to not differ, contrarily from other mammals, this requires further investigation. The decrease in serum AMH concentrations after castration suggests that measurement of this hormone can be used to diagnose bilateral cryptorchid or hemicastrated unilateral cryptorchid animals in this species.

AB - The synthesis of anti-Müllerian Hormone (AMH) by the Sertoli cells in males is crucial for sexual differentiation. There are no studies on AMH in Camelids. The objectives of this research were to 1) compare AMH serum concentrations in prepubertal and adult male alpacas and 2) determine the effect of castration on these concentrations in adult males to provide a validation of a commercial AMH test in alpacas. Serum samples were obtained from 15 prepubertal animals (5 for each age groups of 6, 7 and 8 months) and from 5 adult males (age 5–9 years), pre- and 24 h post-castration. AMH was determined with a quantitative ELISA according to the manufacture's instructions. There was not significant difference (P < 0.05) in AMH level (pg/ml) between pre-pubertal (549.9 ± 120.8, 789.4 ± 172.3, 597.5 ± 177.3 for ages 4, 7, and 8 months, respectively) and adult alpacas (938.7 ± 175.9). In adult males, AMH concentration decreased significantly following castration (P < 0.05) (938.7 ± 383.5 pg/ml) pre-castration, and 222.1 ± 116.5 pg/ml) after castration). There was a positive correlation between testosterone levels and AMH. In conclusion, the quantitative assay used is a reliable test to determine AMH in alpacas. The AMH level in prepubertal and adult alpacas appear to not differ, contrarily from other mammals, this requires further investigation. The decrease in serum AMH concentrations after castration suggests that measurement of this hormone can be used to diagnose bilateral cryptorchid or hemicastrated unilateral cryptorchid animals in this species.

KW - AMH

KW - Camelids

KW - Cryptorchid

UR - http://www.scopus.com/inward/record.url?scp=85030239280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030239280&partnerID=8YFLogxK

U2 - 10.1016/j.theriogenology.2017.09.032

DO - 10.1016/j.theriogenology.2017.09.032

M3 - Article

VL - 105

SP - 174

EP - 177

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

ER -