Effect of a leukocyte-depleting filter in an extracorporeal circuit used for low-flow ischemia and reperfusion of equine jejunum

Linda M. Van Hoogmoed, Jack R. Snyder, Jorge Nieto, Faye A. Harmon, Brenna L. Timmerman

Research output: Contribution to journalArticle

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Abstract

Objective - To determine effect of leukocyte depletion on hematologic, morphologic, and metabolic variables of equine jejunum after induction of arterial low-flow ischemia and reperfusion by use of an extracorporeal circuit. Animals - 14 healthy adult horses. Procedure - A segment of jejunum was surgically removed and maintained in an isolated circuit for 3 hours (control group), arterial flow was reduced to 20% of baseline for 40 minutes followed by 1 hour of reperfusion (low-flow group), or leukocyte depletion was filter-induced, and low-flow ischemia and reperfusion were conducted as in the low-flow control group (filter-treated group). Various metabolic, hemodynamic, and histomorphologic variables were evaluated, including effects of electrical field stimulation and L-N-nitro-arginine-methyl-ester (L-NAME) on contractile activity. Results - The extracorporeal circuit appeared to maintain the jejunum within physiologic limits for an extended period. Low-flow ischemia with reperfusion induced significant differences in various measurements, compared with control specimens. Significant differences were not detected between the low-flow and filter-treated groups. Myeloperoxidase activity was greater in the low-flow group than the control group, whereas a difference was not detected between control and filter-treated groups. Conclusions and Clinical Relevance - The extracorporeal circuit maintained intestine for 3 hours in a physiologic state and may be used for simulation of tissue injury. Leukocyte depletion generally did not attenuate the effects of low-flow ischemia and reperfusion on equine small intestine.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalAmerican Journal of Veterinary Research
Volume62
Issue number1
StatePublished - Jan 2001

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Jejunum
ischemia
jejunum
Horses
Reperfusion
leukocytes
Leukocytes
Ischemia
horses
Control Groups
Peroxidase
Electric Stimulation
Small Intestine
Intestines
Hemodynamics
myeloperoxidase
electric field
hemodynamics
Wounds and Injuries
arginine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Effect of a leukocyte-depleting filter in an extracorporeal circuit used for low-flow ischemia and reperfusion of equine jejunum. / Van Hoogmoed, Linda M.; Snyder, Jack R.; Nieto, Jorge; Harmon, Faye A.; Timmerman, Brenna L.

In: American Journal of Veterinary Research, Vol. 62, No. 1, 01.2001, p. 87-96.

Research output: Contribution to journalArticle

Van Hoogmoed, Linda M. ; Snyder, Jack R. ; Nieto, Jorge ; Harmon, Faye A. ; Timmerman, Brenna L. / Effect of a leukocyte-depleting filter in an extracorporeal circuit used for low-flow ischemia and reperfusion of equine jejunum. In: American Journal of Veterinary Research. 2001 ; Vol. 62, No. 1. pp. 87-96.
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abstract = "Objective - To determine effect of leukocyte depletion on hematologic, morphologic, and metabolic variables of equine jejunum after induction of arterial low-flow ischemia and reperfusion by use of an extracorporeal circuit. Animals - 14 healthy adult horses. Procedure - A segment of jejunum was surgically removed and maintained in an isolated circuit for 3 hours (control group), arterial flow was reduced to 20{\%} of baseline for 40 minutes followed by 1 hour of reperfusion (low-flow group), or leukocyte depletion was filter-induced, and low-flow ischemia and reperfusion were conducted as in the low-flow control group (filter-treated group). Various metabolic, hemodynamic, and histomorphologic variables were evaluated, including effects of electrical field stimulation and L-N-nitro-arginine-methyl-ester (L-NAME) on contractile activity. Results - The extracorporeal circuit appeared to maintain the jejunum within physiologic limits for an extended period. Low-flow ischemia with reperfusion induced significant differences in various measurements, compared with control specimens. Significant differences were not detected between the low-flow and filter-treated groups. Myeloperoxidase activity was greater in the low-flow group than the control group, whereas a difference was not detected between control and filter-treated groups. Conclusions and Clinical Relevance - The extracorporeal circuit maintained intestine for 3 hours in a physiologic state and may be used for simulation of tissue injury. Leukocyte depletion generally did not attenuate the effects of low-flow ischemia and reperfusion on equine small intestine.",
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