Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity

R. T. Kawahata, C. A. Holmberg, Bennie Osburn, L. F. Chuang, R. Y. Chuang

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.

Original languageEnglish (US)
Pages (from-to)503-506
Number of pages4
JournalMolecular Pharmacology
Volume18
Issue number3
StatePublished - Dec 1 1980

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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    Kawahata, R. T., Holmberg, C. A., Osburn, B., Chuang, L. F., & Chuang, R. Y. (1980). Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity. Molecular Pharmacology, 18(3), 503-506.