Abstract
6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 503-506 |
| Number of pages | 4 |
| Journal | Molecular Pharmacology |
| Volume | 18 |
| Issue number | 3 |
| State | Published - Dec 1 1980 |
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ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology
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Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity. / Kawahata, R. T.; Holmberg, C. A.; Osburn, Bennie; Chuang, L. F.; Chuang, R. Y.
In: Molecular Pharmacology, Vol. 18, No. 3, 01.12.1980, p. 503-506.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity
AU - Kawahata, R. T.
AU - Holmberg, C. A.
AU - Osburn, Bennie
AU - Chuang, L. F.
AU - Chuang, R. Y.
PY - 1980/12/1
Y1 - 1980/12/1
N2 - 6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.
AB - 6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.
UR - http://www.scopus.com/inward/record.url?scp=0019179114&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019179114&partnerID=8YFLogxK
M3 - Article
VL - 18
SP - 503
EP - 506
JO - Molecular Pharmacology
JF - Molecular Pharmacology
SN - 0026-895X
IS - 3
ER -