Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity

R. T. Kawahata, C. A. Holmberg, Bennie Osburn, L. F. Chuang, R. Y. Chuang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.

Original languageEnglish (US)
Pages (from-to)503-506
Number of pages4
JournalMolecular Pharmacology
Volume18
Issue number3
StatePublished - Dec 1 1980

Fingerprint

Ribonucleotides
6-Mercaptopurine
DNA-Directed RNA Polymerases
RNA
Ribonucleosides
Guanosine Triphosphate
Antineoplastic Agents
Chickens
Escherichia coli

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Kawahata, R. T., Holmberg, C. A., Osburn, B., Chuang, L. F., & Chuang, R. Y. (1980). Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity. Molecular Pharmacology, 18(3), 503-506.

Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity. / Kawahata, R. T.; Holmberg, C. A.; Osburn, Bennie; Chuang, L. F.; Chuang, R. Y.

In: Molecular Pharmacology, Vol. 18, No. 3, 01.12.1980, p. 503-506.

Research output: Contribution to journalArticle

Kawahata, RT, Holmberg, CA, Osburn, B, Chuang, LF & Chuang, RY 1980, 'Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity', Molecular Pharmacology, vol. 18, no. 3, pp. 503-506.
Kawahata RT, Holmberg CA, Osburn B, Chuang LF, Chuang RY. Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity. Molecular Pharmacology. 1980 Dec 1;18(3):503-506.
Kawahata, R. T. ; Holmberg, C. A. ; Osburn, Bennie ; Chuang, L. F. ; Chuang, R. Y. / Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity. In: Molecular Pharmacology. 1980 ; Vol. 18, No. 3. pp. 503-506.
@article{f846723306304afb8c680d8b658de842,
title = "Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity",
abstract = "6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.",
author = "Kawahata, {R. T.} and Holmberg, {C. A.} and Bennie Osburn and Chuang, {L. F.} and Chuang, {R. Y.}",
year = "1980",
month = "12",
day = "1",
language = "English (US)",
volume = "18",
pages = "503--506",
journal = "Molecular Pharmacology",
issn = "0026-895X",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "3",

}

TY - JOUR

T1 - Effect of 6-mercaptopurine ribonucleotides on DNA-dependent RNA polymerase activity

AU - Kawahata, R. T.

AU - Holmberg, C. A.

AU - Osburn, Bennie

AU - Chuang, L. F.

AU - Chuang, R. Y.

PY - 1980/12/1

Y1 - 1980/12/1

N2 - 6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.

AB - 6-Mercaptopurine ribonucleoside 5'-triphosphate was found to be an inhibitor of DNA-dependent RNA polymerases from Escherichia coli and chicken myeloblastosis cells. The inhibitor appears to compete with GTP for its binding to RNA polymerase, resulting in a reduction of RNA chain initiations. Neither 6-mercaptopurine ribonucleoside nor its monophosphate derivative caused the inhibition. These studies suggest that the antineoplastic action of 6-mercaptopurine may in part be owing to the inhibition of the activity of DNA-dependent RNA polymerase, resulting in an inhibition of cellular RNA synthesis.

UR - http://www.scopus.com/inward/record.url?scp=0019179114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019179114&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 503

EP - 506

JO - Molecular Pharmacology

JF - Molecular Pharmacology

SN - 0026-895X

IS - 3

ER -