Effect of 527 nm laser light on collagen crosslinks: Implications for tissue welding

W. IV Small, K. M. Reiser, N. J. Heredia, D. J. Maitland, L. B. Da Silva, Dennis L Matthews

Research output: Contribution to journalArticlepeer-review


Laser-induced changes in native collagen crosslinking may impact the success of laser tissue fusion. To investigate the influence of laser exposure on crosslinking, a systematic in vitro study was performed using a porcine carotid artery model. Tissues were irradiated using a 527 nm solid state laser at various powers (0.5, 1.0, 3.0, 5.0, 8.0 W) for different durations (5, 10, 15, 30 s); no absorbing chromophores or solders were used. The difunctional crosslink HLNL, its dihydroxylated analogue DHLNL and the trifunctional product of DHLNL, OHP were quantified for treated and native tissues. Laser power and duration had variable effects on HLNL, which generally increased or remained unchanged. DHLNL was significantly elevated above native levels for laser exposed tissue; both power and duration were independently correlated with the increase. OHP was significantly decreased compared to native controls; the effect was strongly correlated with duration, but not power. Examination of the precursor-product relationship suggests that under some conditions OHP was degraded to the precursor stage which may account for the increase in DHLNL, while under other conditions it may have degraded beyond this stage. Though mature crosslinks may be reduced, optimal laser exposure conditions may exist which do not destroy the precursors for a given tissue, Laser-induced formation and destruction of covalent crosslinks may impact the strength and stability of laser welded tissues.

Original languageEnglish (US)
Pages (from-to)151-162
Number of pages12
JournalLasers in the Life Sciences
Issue number3
StatePublished - 1998
Externally publishedYes


  • Collagen crosslinks
  • Laser tissue welding
  • Porcine carotid artery
  • Tissue fusion

ASJC Scopus subject areas

  • Medicine (miscellaneous)


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