Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens

Bobbie R. Erickson; Tara K. Sealy; Tim Flietstra; Laura Morgan; Brima Kargbo; Victor E. Matt-Lebby; Aridth Gibbons; Ayan K. Chakrabarti; James Graziano; Lance Presser; Mike Flint; Brian H. Bird; Shelley Brown; John D. Klena; DIanna M. Blau; Aaron Brault; Jessica A. Belser; Johanna S. Salzer; Amy J. Schuh; Michael Lo; Marko Zivcec; Rachael A. Priestley; Meredith Pyle; Christin Goodman; Scott Bearden; Brian R. Amman; Alison Basile; Éric Bergeron; Michael D. Bowen; Kimberly A. Dodd; Molly M. Freeman; Laura K. McMullan; Christopher D. Paddock; Brandy J. Russell; Angela J. Sanchez; Jonathan S. Towner; David Wang; Galina E. Zemtsova; Robyn A. Stoddard; Maryann Turnsek; Lisa Wiggleton Guerrero; Shannon L. Emery; Janae Stovall; Markus H. Kainulainen; Jamie L. Perniciaro; Slavica Mijatovic-Rustempasic; Gulchekhra Shakirova; Jörn Winter; Christopher Sexton; Feng Liu; Kimetha Slater; Raydel Anderson; Lauren Andersen; Cheng Feng Chiang; Wen Pin Tzeng; Samuel J. Crowe; Matthew J. Maenner; Christina F. Spiropoulou; Stuart T. Nichol; Ute Ströher

doi:10.1093/infdis/jiw296

Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens

Bobbie R. Erickson, Tara K. Sealy, Tim Flietstra, Laura Morgan, Brima Kargbo, Victor E. Matt-Lebby, Aridth Gibbons, Ayan K. Chakrabarti, James Graziano, Lance Presser, Mike Flint, Brian H. Bird, Shelley Brown, John D. Klena, DIanna M. Blau, Aaron Brault, Jessica A. Belser, Johanna S. Salzer, Amy J. Schuh, Michael LoMarko Zivcec, Rachael A. Priestley, Meredith Pyle, Christin Goodman, Scott Bearden, Brian R. Amman, Alison Basile, Éric Bergeron, Michael D. Bowen, Kimberly A. Dodd, Molly M. Freeman, Laura K. McMullan, Christopher D. Paddock, Brandy J. Russell, Angela J. Sanchez, Jonathan S. Towner, David Wang, Galina E. Zemtsova, Robyn A. Stoddard, Maryann Turnsek, Lisa Wiggleton Guerrero, Shannon L. Emery, Janae Stovall, Markus H. Kainulainen, Jamie L. Perniciaro, Slavica Mijatovic-Rustempasic, Gulchekhra Shakirova, Jörn Winter, Christopher Sexton, Feng Liu, Kimetha Slater, Raydel Anderson, Lauren Andersen, Cheng Feng Chiang, Wen Pin Tzeng, Samuel J. Crowe, Matthew J. Maenner, Christina F. Spiropoulou, Stuart T. Nichol, Ute Ströher

Research output: Contribution to journal › Article

11 Scopus citations

Abstract

During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.

Original language	English (US)
Pages (from-to)	S258-S262
Journal	Journal of Infectious Diseases
Volume	214
DOIs	https://doi.org/10.1093/infdis/jiw296
State	Published - Oct 15 2016
Externally published	Yes

Keywords

Ebola virus
Endogenous control
Housekeeping gene
QRT-PCR
West Africa

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

Access to Document

10.1093/infdis/jiw296

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Erickson, B. R., Sealy, T. K., Flietstra, T., Morgan, L., Kargbo, B., Matt-Lebby, V. E., Gibbons, A., Chakrabarti, A. K., Graziano, J., Presser, L., Flint, M., Bird, B. H., Brown, S., Klena, J. D., Blau, DI. M., Brault, A., Belser, J. A., Salzer, J. S., Schuh, A. J., ... Ströher, U. (2016). Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens. Journal of Infectious Diseases, 214, S258-S262. https://doi.org/10.1093/infdis/jiw296

Ebola Virus Disease Diagnostics, Sierra Leone : Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens. / Erickson, Bobbie R.; Sealy, Tara K.; Flietstra, Tim; Morgan, Laura; Kargbo, Brima; Matt-Lebby, Victor E.; Gibbons, Aridth; Chakrabarti, Ayan K.; Graziano, James; Presser, Lance; Flint, Mike; Bird, Brian H.; Brown, Shelley; Klena, John D.; Blau, DIanna M.; Brault, Aaron; Belser, Jessica A.; Salzer, Johanna S.; Schuh, Amy J.; Lo, Michael; Zivcec, Marko; Priestley, Rachael A.; Pyle, Meredith; Goodman, Christin; Bearden, Scott; Amman, Brian R.; Basile, Alison; Bergeron, Éric; Bowen, Michael D.; Dodd, Kimberly A.; Freeman, Molly M.; McMullan, Laura K.; Paddock, Christopher D.; Russell, Brandy J.; Sanchez, Angela J.; Towner, Jonathan S.; Wang, David; Zemtsova, Galina E.; Stoddard, Robyn A.; Turnsek, Maryann; Guerrero, Lisa Wiggleton; Emery, Shannon L.; Stovall, Janae; Kainulainen, Markus H.; Perniciaro, Jamie L.; Mijatovic-Rustempasic, Slavica; Shakirova, Gulchekhra; Winter, Jörn; Sexton, Christopher; Liu, Feng; Slater, Kimetha; Anderson, Raydel; Andersen, Lauren; Chiang, Cheng Feng; Tzeng, Wen Pin; Crowe, Samuel J.; Maenner, Matthew J.; Spiropoulou, Christina F.; Nichol, Stuart T.; Ströher, Ute.

In: Journal of Infectious Diseases, Vol. 214, 15.10.2016, p. S258-S262.

Research output: Contribution to journal › Article

Erickson, BR, Sealy, TK, Flietstra, T, Morgan, L, Kargbo, B, Matt-Lebby, VE, Gibbons, A, Chakrabarti, AK, Graziano, J, Presser, L, Flint, M, Bird, BH, Brown, S, Klena, JD, Blau, DIM, Brault, A, Belser, JA, Salzer, JS, Schuh, AJ, Lo, M, Zivcec, M, Priestley, RA, Pyle, M, Goodman, C, Bearden, S, Amman, BR, Basile, A, Bergeron, É, Bowen, MD, Dodd, KA, Freeman, MM, McMullan, LK, Paddock, CD, Russell, BJ, Sanchez, AJ, Towner, JS, Wang, D, Zemtsova, GE, Stoddard, RA, Turnsek, M, Guerrero, LW, Emery, SL, Stovall, J, Kainulainen, MH, Perniciaro, JL, Mijatovic-Rustempasic, S, Shakirova, G, Winter, J, Sexton, C, Liu, F, Slater, K, Anderson, R, Andersen, L, Chiang, CF, Tzeng, WP, Crowe, SJ, Maenner, MJ, Spiropoulou, CF, Nichol, ST & Ströher, U 2016, 'Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens', Journal of Infectious Diseases, vol. 214, pp. S258-S262. https://doi.org/10.1093/infdis/jiw296

Erickson, Bobbie R. ; Sealy, Tara K. ; Flietstra, Tim ; Morgan, Laura ; Kargbo, Brima ; Matt-Lebby, Victor E. ; Gibbons, Aridth ; Chakrabarti, Ayan K. ; Graziano, James ; Presser, Lance ; Flint, Mike ; Bird, Brian H. ; Brown, Shelley ; Klena, John D. ; Blau, DIanna M. ; Brault, Aaron ; Belser, Jessica A. ; Salzer, Johanna S. ; Schuh, Amy J. ; Lo, Michael ; Zivcec, Marko ; Priestley, Rachael A. ; Pyle, Meredith ; Goodman, Christin ; Bearden, Scott ; Amman, Brian R. ; Basile, Alison ; Bergeron, Éric ; Bowen, Michael D. ; Dodd, Kimberly A. ; Freeman, Molly M. ; McMullan, Laura K. ; Paddock, Christopher D. ; Russell, Brandy J. ; Sanchez, Angela J. ; Towner, Jonathan S. ; Wang, David ; Zemtsova, Galina E. ; Stoddard, Robyn A. ; Turnsek, Maryann ; Guerrero, Lisa Wiggleton ; Emery, Shannon L. ; Stovall, Janae ; Kainulainen, Markus H. ; Perniciaro, Jamie L. ; Mijatovic-Rustempasic, Slavica ; Shakirova, Gulchekhra ; Winter, Jörn ; Sexton, Christopher ; Liu, Feng ; Slater, Kimetha ; Anderson, Raydel ; Andersen, Lauren ; Chiang, Cheng Feng ; Tzeng, Wen Pin ; Crowe, Samuel J. ; Maenner, Matthew J. ; Spiropoulou, Christina F. ; Nichol, Stuart T. ; Ströher, Ute. / Ebola Virus Disease Diagnostics, Sierra Leone : Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens. In: Journal of Infectious Diseases. 2016 ; Vol. 214. pp. S258-S262.

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abstract = "During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.",

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AU - Sealy, Tara K.

AU - Flietstra, Tim

AU - Morgan, Laura

AU - Kargbo, Brima

AU - Matt-Lebby, Victor E.

AU - Gibbons, Aridth

AU - Chakrabarti, Ayan K.

AU - Graziano, James

AU - Presser, Lance

AU - Flint, Mike

AU - Bird, Brian H.

AU - Brown, Shelley

AU - Klena, John D.

AU - Blau, DIanna M.

AU - Brault, Aaron

AU - Belser, Jessica A.

AU - Salzer, Johanna S.

AU - Schuh, Amy J.

AU - Lo, Michael

AU - Zivcec, Marko

AU - Priestley, Rachael A.

AU - Pyle, Meredith

AU - Goodman, Christin

AU - Bearden, Scott

AU - Amman, Brian R.

AU - Basile, Alison

AU - Bergeron, Éric

AU - Bowen, Michael D.

AU - Dodd, Kimberly A.

AU - Freeman, Molly M.

AU - McMullan, Laura K.

AU - Paddock, Christopher D.

AU - Russell, Brandy J.

AU - Sanchez, Angela J.

AU - Towner, Jonathan S.

AU - Wang, David

AU - Zemtsova, Galina E.

AU - Stoddard, Robyn A.

AU - Turnsek, Maryann

AU - Guerrero, Lisa Wiggleton

AU - Emery, Shannon L.

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AU - Kainulainen, Markus H.

AU - Perniciaro, Jamie L.

AU - Mijatovic-Rustempasic, Slavica

AU - Shakirova, Gulchekhra

AU - Winter, Jörn

AU - Sexton, Christopher

AU - Liu, Feng

AU - Slater, Kimetha

AU - Anderson, Raydel

AU - Andersen, Lauren

AU - Chiang, Cheng Feng

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KW - Housekeeping gene

KW - QRT-PCR

KW - West Africa

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