Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens

Bobbie R. Erickson, Tara K. Sealy, Tim Flietstra, Laura Morgan, Brima Kargbo, Victor E. Matt-Lebby, Aridth Gibbons, Ayan K. Chakrabarti, James Graziano, Lance Presser, Mike Flint, Brian H. Bird, Shelley Brown, John D. Klena, DIanna M. Blau, Aaron Brault, Jessica A. Belser, Johanna S. Salzer, Amy J. Schuh, Michael LoMarko Zivcec, Rachael A. Priestley, Meredith Pyle, Christin Goodman, Scott Bearden, Brian R. Amman, Alison Basile, Éric Bergeron, Michael D. Bowen, Kimberly A. Dodd, Molly M. Freeman, Laura K. McMullan, Christopher D. Paddock, Brandy J. Russell, Angela J. Sanchez, Jonathan S. Towner, David Wang, Galina E. Zemtsova, Robyn A. Stoddard, Maryann Turnsek, Lisa Wiggleton Guerrero, Shannon L. Emery, Janae Stovall, Markus H. Kainulainen, Jamie L. Perniciaro, Slavica Mijatovic-Rustempasic, Gulchekhra Shakirova, Jörn Winter, Christopher Sexton, Feng Liu, Kimetha Slater, Raydel Anderson, Lauren Andersen, Cheng Feng Chiang, Wen Pin Tzeng, Samuel J. Crowe, Matthew J. Maenner, Christina F. Spiropoulou, Stuart T. Nichol, Ute Ströher

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.

Original languageEnglish (US)
Pages (from-to)S258-S262
JournalJournal of Infectious Diseases
Volume214
DOIs
StatePublished - Oct 15 2016
Externally publishedYes

Keywords

  • Ebola virus
  • Endogenous control
  • Housekeeping gene
  • QRT-PCR
  • West Africa

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Erickson, B. R., Sealy, T. K., Flietstra, T., Morgan, L., Kargbo, B., Matt-Lebby, V. E., Gibbons, A., Chakrabarti, A. K., Graziano, J., Presser, L., Flint, M., Bird, B. H., Brown, S., Klena, J. D., Blau, DI. M., Brault, A., Belser, J. A., Salzer, J. S., Schuh, A. J., ... Ströher, U. (2016). Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens. Journal of Infectious Diseases, 214, S258-S262. https://doi.org/10.1093/infdis/jiw296