Ebola RNA persistence in semen of ebola virus disease survivors — Final report

G. F. Deen, N. Broutet, W. Xu, B. Knust, F. R. Sesay, S. L.R. McDonald, E. Ervin, J. E. Marrinan, P. Gaillard, N. Habib, H. Liu, W. Liu, A. E. Thorson, F. Yamba, T. A. Massaquoi, F. James, A. Ariyarajah, C. Ross, K. Bernstein, A. Coursier & 39 others J. Klena, M. Carino, A. H. Wurie, Y. Zhang, M. S. Dumbuya, N. Abad, B. Idriss, T. Wi, S. D. Bennett, T. Davies, F. K. Ebrahim, E. Meites, D. Naidoo, S. J. Smith, P. Ongpin, T. Malik, A. Banerjee, B. R. Erickson, Y. Liu, Y. Liu, K. Xu, Aaron Brault, K. N. Durski, J. Winter, T. Sealy, S. T. Nichol, M. Lamunu, J. Bangura, S. Landoulsi, A. Jambai, O. Morgan, G. Wu, M. Liang, Q. Su, Y. Lan, Y. Hao, P. Formenty, U. Ströher, F. Sahr

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

BACKGROUND: Ebola virus has been detected in the semen of men after their recovery from Ebola virus disease (EVD). We report the presence of Ebola virus RNA in semen in a cohort of survivors of EVD in Sierra Leone. METHODS: We enrolled a convenience sample of 220 adult male survivors of EVD in Sierra Leone, at various times after discharge from an Ebola treatment unit (ETU), in two phases (100 participants were in phase 1, and 120 in phase 2). Semen specimens obtained at baseline were tested by means of a quantitative reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP and VP40 (in phase 1) or NP and GP (in phase 2). This study did not evaluate directly the risk of sexual transmission of EVD. RESULTS: Of 210 participants who provided an initial semen specimen for analysis, 57 (27%) had positive results on quantitative RT-PCR. Ebola virus RNA was detected in the semen of all 7 men with a specimen obtained within 3 months after ETU discharge, in 26 of 42 (62%) with a specimen obtained at 4 to 6 months, in 15 of 60 (25%) with a specimen obtained at 7 to 9 months, in 4 of 26 (15%) with a specimen obtained at 10 to 12 months, in 4 of 38 (11%) with a specimen obtained at 13 to 15 months, in 1 of 25 (4%) with a specimen obtained at 16 to 18 months, and in no men with a specimen obtained at 19 months or later. Among the 46 participants with a positive result in phase 1, the median baseline cycle-threshold values (higher values indicate lower RNA values) for the NP and VP40 targets were lower within 3 months after ETU discharge (32.4 and 31.3, respectively; in 7 men) than at 4 to 6 months (34.3 and 33.1; in 25), at 7 to 9 months (37.4 and 36.6; in 13), and at 10 to 12 months (37.7 and 36.9; in 1). In phase 2, a total of 11 participants had positive results for NP and GP targets (samples obtained at 4.1 to 15.7 months after ETU discharge); cycle-threshold values ranged from 32.7 to 38.0 for NP and from 31.1 to 37.7 for GP. CONCLUSIONS: These data showed the long-term presence of Ebola virus RNA in semen and declining persistence with increasing time after ETU discharge.

Original languageEnglish (US)
Pages (from-to)1428-1437
Number of pages10
JournalNew England Journal of Medicine
Volume377
Issue number15
DOIs
StatePublished - Oct 12 2017
Externally publishedYes

Fingerprint

Ebola Hemorrhagic Fever
Ebolavirus
Semen
Survivors
RNA
Sierra Leone
Therapeutics
Semen Analysis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Deen, G. F., Broutet, N., Xu, W., Knust, B., Sesay, F. R., McDonald, S. L. R., ... Sahr, F. (2017). Ebola RNA persistence in semen of ebola virus disease survivors — Final report. New England Journal of Medicine, 377(15), 1428-1437. https://doi.org/10.1056/NEJMoa1511410

Ebola RNA persistence in semen of ebola virus disease survivors — Final report. / Deen, G. F.; Broutet, N.; Xu, W.; Knust, B.; Sesay, F. R.; McDonald, S. L.R.; Ervin, E.; Marrinan, J. E.; Gaillard, P.; Habib, N.; Liu, H.; Liu, W.; Thorson, A. E.; Yamba, F.; Massaquoi, T. A.; James, F.; Ariyarajah, A.; Ross, C.; Bernstein, K.; Coursier, A.; Klena, J.; Carino, M.; Wurie, A. H.; Zhang, Y.; Dumbuya, M. S.; Abad, N.; Idriss, B.; Wi, T.; Bennett, S. D.; Davies, T.; Ebrahim, F. K.; Meites, E.; Naidoo, D.; Smith, S. J.; Ongpin, P.; Malik, T.; Banerjee, A.; Erickson, B. R.; Liu, Y.; Liu, Y.; Xu, K.; Brault, Aaron; Durski, K. N.; Winter, J.; Sealy, T.; Nichol, S. T.; Lamunu, M.; Bangura, J.; Landoulsi, S.; Jambai, A.; Morgan, O.; Wu, G.; Liang, M.; Su, Q.; Lan, Y.; Hao, Y.; Formenty, P.; Ströher, U.; Sahr, F.

In: New England Journal of Medicine, Vol. 377, No. 15, 12.10.2017, p. 1428-1437.

Research output: Contribution to journalArticle

Deen, GF, Broutet, N, Xu, W, Knust, B, Sesay, FR, McDonald, SLR, Ervin, E, Marrinan, JE, Gaillard, P, Habib, N, Liu, H, Liu, W, Thorson, AE, Yamba, F, Massaquoi, TA, James, F, Ariyarajah, A, Ross, C, Bernstein, K, Coursier, A, Klena, J, Carino, M, Wurie, AH, Zhang, Y, Dumbuya, MS, Abad, N, Idriss, B, Wi, T, Bennett, SD, Davies, T, Ebrahim, FK, Meites, E, Naidoo, D, Smith, SJ, Ongpin, P, Malik, T, Banerjee, A, Erickson, BR, Liu, Y, Liu, Y, Xu, K, Brault, A, Durski, KN, Winter, J, Sealy, T, Nichol, ST, Lamunu, M, Bangura, J, Landoulsi, S, Jambai, A, Morgan, O, Wu, G, Liang, M, Su, Q, Lan, Y, Hao, Y, Formenty, P, Ströher, U & Sahr, F 2017, 'Ebola RNA persistence in semen of ebola virus disease survivors — Final report', New England Journal of Medicine, vol. 377, no. 15, pp. 1428-1437. https://doi.org/10.1056/NEJMoa1511410
Deen GF, Broutet N, Xu W, Knust B, Sesay FR, McDonald SLR et al. Ebola RNA persistence in semen of ebola virus disease survivors — Final report. New England Journal of Medicine. 2017 Oct 12;377(15):1428-1437. https://doi.org/10.1056/NEJMoa1511410
Deen, G. F. ; Broutet, N. ; Xu, W. ; Knust, B. ; Sesay, F. R. ; McDonald, S. L.R. ; Ervin, E. ; Marrinan, J. E. ; Gaillard, P. ; Habib, N. ; Liu, H. ; Liu, W. ; Thorson, A. E. ; Yamba, F. ; Massaquoi, T. A. ; James, F. ; Ariyarajah, A. ; Ross, C. ; Bernstein, K. ; Coursier, A. ; Klena, J. ; Carino, M. ; Wurie, A. H. ; Zhang, Y. ; Dumbuya, M. S. ; Abad, N. ; Idriss, B. ; Wi, T. ; Bennett, S. D. ; Davies, T. ; Ebrahim, F. K. ; Meites, E. ; Naidoo, D. ; Smith, S. J. ; Ongpin, P. ; Malik, T. ; Banerjee, A. ; Erickson, B. R. ; Liu, Y. ; Liu, Y. ; Xu, K. ; Brault, Aaron ; Durski, K. N. ; Winter, J. ; Sealy, T. ; Nichol, S. T. ; Lamunu, M. ; Bangura, J. ; Landoulsi, S. ; Jambai, A. ; Morgan, O. ; Wu, G. ; Liang, M. ; Su, Q. ; Lan, Y. ; Hao, Y. ; Formenty, P. ; Ströher, U. ; Sahr, F. / Ebola RNA persistence in semen of ebola virus disease survivors — Final report. In: New England Journal of Medicine. 2017 ; Vol. 377, No. 15. pp. 1428-1437.
@article{b41bc292940148bab6d46eca790fd7e6,
title = "Ebola RNA persistence in semen of ebola virus disease survivors — Final report",
abstract = "BACKGROUND: Ebola virus has been detected in the semen of men after their recovery from Ebola virus disease (EVD). We report the presence of Ebola virus RNA in semen in a cohort of survivors of EVD in Sierra Leone. METHODS: We enrolled a convenience sample of 220 adult male survivors of EVD in Sierra Leone, at various times after discharge from an Ebola treatment unit (ETU), in two phases (100 participants were in phase 1, and 120 in phase 2). Semen specimens obtained at baseline were tested by means of a quantitative reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP and VP40 (in phase 1) or NP and GP (in phase 2). This study did not evaluate directly the risk of sexual transmission of EVD. RESULTS: Of 210 participants who provided an initial semen specimen for analysis, 57 (27{\%}) had positive results on quantitative RT-PCR. Ebola virus RNA was detected in the semen of all 7 men with a specimen obtained within 3 months after ETU discharge, in 26 of 42 (62{\%}) with a specimen obtained at 4 to 6 months, in 15 of 60 (25{\%}) with a specimen obtained at 7 to 9 months, in 4 of 26 (15{\%}) with a specimen obtained at 10 to 12 months, in 4 of 38 (11{\%}) with a specimen obtained at 13 to 15 months, in 1 of 25 (4{\%}) with a specimen obtained at 16 to 18 months, and in no men with a specimen obtained at 19 months or later. Among the 46 participants with a positive result in phase 1, the median baseline cycle-threshold values (higher values indicate lower RNA values) for the NP and VP40 targets were lower within 3 months after ETU discharge (32.4 and 31.3, respectively; in 7 men) than at 4 to 6 months (34.3 and 33.1; in 25), at 7 to 9 months (37.4 and 36.6; in 13), and at 10 to 12 months (37.7 and 36.9; in 1). In phase 2, a total of 11 participants had positive results for NP and GP targets (samples obtained at 4.1 to 15.7 months after ETU discharge); cycle-threshold values ranged from 32.7 to 38.0 for NP and from 31.1 to 37.7 for GP. CONCLUSIONS: These data showed the long-term presence of Ebola virus RNA in semen and declining persistence with increasing time after ETU discharge.",
author = "Deen, {G. F.} and N. Broutet and W. Xu and B. Knust and Sesay, {F. R.} and McDonald, {S. L.R.} and E. Ervin and Marrinan, {J. E.} and P. Gaillard and N. Habib and H. Liu and W. Liu and Thorson, {A. E.} and F. Yamba and Massaquoi, {T. A.} and F. James and A. Ariyarajah and C. Ross and K. Bernstein and A. Coursier and J. Klena and M. Carino and Wurie, {A. H.} and Y. Zhang and Dumbuya, {M. S.} and N. Abad and B. Idriss and T. Wi and Bennett, {S. D.} and T. Davies and Ebrahim, {F. K.} and E. Meites and D. Naidoo and Smith, {S. J.} and P. Ongpin and T. Malik and A. Banerjee and Erickson, {B. R.} and Y. Liu and Y. Liu and K. Xu and Aaron Brault and Durski, {K. N.} and J. Winter and T. Sealy and Nichol, {S. T.} and M. Lamunu and J. Bangura and S. Landoulsi and A. Jambai and O. Morgan and G. Wu and M. Liang and Q. Su and Y. Lan and Y. Hao and P. Formenty and U. Str{\"o}her and F. Sahr",
year = "2017",
month = "10",
day = "12",
doi = "10.1056/NEJMoa1511410",
language = "English (US)",
volume = "377",
pages = "1428--1437",
journal = "New England Journal of Medicine",
issn = "0028-4793",
publisher = "Massachussetts Medical Society",
number = "15",

}

TY - JOUR

T1 - Ebola RNA persistence in semen of ebola virus disease survivors — Final report

AU - Deen, G. F.

AU - Broutet, N.

AU - Xu, W.

AU - Knust, B.

AU - Sesay, F. R.

AU - McDonald, S. L.R.

AU - Ervin, E.

AU - Marrinan, J. E.

AU - Gaillard, P.

AU - Habib, N.

AU - Liu, H.

AU - Liu, W.

AU - Thorson, A. E.

AU - Yamba, F.

AU - Massaquoi, T. A.

AU - James, F.

AU - Ariyarajah, A.

AU - Ross, C.

AU - Bernstein, K.

AU - Coursier, A.

AU - Klena, J.

AU - Carino, M.

AU - Wurie, A. H.

AU - Zhang, Y.

AU - Dumbuya, M. S.

AU - Abad, N.

AU - Idriss, B.

AU - Wi, T.

AU - Bennett, S. D.

AU - Davies, T.

AU - Ebrahim, F. K.

AU - Meites, E.

AU - Naidoo, D.

AU - Smith, S. J.

AU - Ongpin, P.

AU - Malik, T.

AU - Banerjee, A.

AU - Erickson, B. R.

AU - Liu, Y.

AU - Liu, Y.

AU - Xu, K.

AU - Brault, Aaron

AU - Durski, K. N.

AU - Winter, J.

AU - Sealy, T.

AU - Nichol, S. T.

AU - Lamunu, M.

AU - Bangura, J.

AU - Landoulsi, S.

AU - Jambai, A.

AU - Morgan, O.

AU - Wu, G.

AU - Liang, M.

AU - Su, Q.

AU - Lan, Y.

AU - Hao, Y.

AU - Formenty, P.

AU - Ströher, U.

AU - Sahr, F.

PY - 2017/10/12

Y1 - 2017/10/12

N2 - BACKGROUND: Ebola virus has been detected in the semen of men after their recovery from Ebola virus disease (EVD). We report the presence of Ebola virus RNA in semen in a cohort of survivors of EVD in Sierra Leone. METHODS: We enrolled a convenience sample of 220 adult male survivors of EVD in Sierra Leone, at various times after discharge from an Ebola treatment unit (ETU), in two phases (100 participants were in phase 1, and 120 in phase 2). Semen specimens obtained at baseline were tested by means of a quantitative reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP and VP40 (in phase 1) or NP and GP (in phase 2). This study did not evaluate directly the risk of sexual transmission of EVD. RESULTS: Of 210 participants who provided an initial semen specimen for analysis, 57 (27%) had positive results on quantitative RT-PCR. Ebola virus RNA was detected in the semen of all 7 men with a specimen obtained within 3 months after ETU discharge, in 26 of 42 (62%) with a specimen obtained at 4 to 6 months, in 15 of 60 (25%) with a specimen obtained at 7 to 9 months, in 4 of 26 (15%) with a specimen obtained at 10 to 12 months, in 4 of 38 (11%) with a specimen obtained at 13 to 15 months, in 1 of 25 (4%) with a specimen obtained at 16 to 18 months, and in no men with a specimen obtained at 19 months or later. Among the 46 participants with a positive result in phase 1, the median baseline cycle-threshold values (higher values indicate lower RNA values) for the NP and VP40 targets were lower within 3 months after ETU discharge (32.4 and 31.3, respectively; in 7 men) than at 4 to 6 months (34.3 and 33.1; in 25), at 7 to 9 months (37.4 and 36.6; in 13), and at 10 to 12 months (37.7 and 36.9; in 1). In phase 2, a total of 11 participants had positive results for NP and GP targets (samples obtained at 4.1 to 15.7 months after ETU discharge); cycle-threshold values ranged from 32.7 to 38.0 for NP and from 31.1 to 37.7 for GP. CONCLUSIONS: These data showed the long-term presence of Ebola virus RNA in semen and declining persistence with increasing time after ETU discharge.

AB - BACKGROUND: Ebola virus has been detected in the semen of men after their recovery from Ebola virus disease (EVD). We report the presence of Ebola virus RNA in semen in a cohort of survivors of EVD in Sierra Leone. METHODS: We enrolled a convenience sample of 220 adult male survivors of EVD in Sierra Leone, at various times after discharge from an Ebola treatment unit (ETU), in two phases (100 participants were in phase 1, and 120 in phase 2). Semen specimens obtained at baseline were tested by means of a quantitative reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP and VP40 (in phase 1) or NP and GP (in phase 2). This study did not evaluate directly the risk of sexual transmission of EVD. RESULTS: Of 210 participants who provided an initial semen specimen for analysis, 57 (27%) had positive results on quantitative RT-PCR. Ebola virus RNA was detected in the semen of all 7 men with a specimen obtained within 3 months after ETU discharge, in 26 of 42 (62%) with a specimen obtained at 4 to 6 months, in 15 of 60 (25%) with a specimen obtained at 7 to 9 months, in 4 of 26 (15%) with a specimen obtained at 10 to 12 months, in 4 of 38 (11%) with a specimen obtained at 13 to 15 months, in 1 of 25 (4%) with a specimen obtained at 16 to 18 months, and in no men with a specimen obtained at 19 months or later. Among the 46 participants with a positive result in phase 1, the median baseline cycle-threshold values (higher values indicate lower RNA values) for the NP and VP40 targets were lower within 3 months after ETU discharge (32.4 and 31.3, respectively; in 7 men) than at 4 to 6 months (34.3 and 33.1; in 25), at 7 to 9 months (37.4 and 36.6; in 13), and at 10 to 12 months (37.7 and 36.9; in 1). In phase 2, a total of 11 participants had positive results for NP and GP targets (samples obtained at 4.1 to 15.7 months after ETU discharge); cycle-threshold values ranged from 32.7 to 38.0 for NP and from 31.1 to 37.7 for GP. CONCLUSIONS: These data showed the long-term presence of Ebola virus RNA in semen and declining persistence with increasing time after ETU discharge.

UR - http://www.scopus.com/inward/record.url?scp=85031034733&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031034733&partnerID=8YFLogxK

U2 - 10.1056/NEJMoa1511410

DO - 10.1056/NEJMoa1511410

M3 - Article

VL - 377

SP - 1428

EP - 1437

JO - New England Journal of Medicine

JF - New England Journal of Medicine

SN - 0028-4793

IS - 15

ER -