Earning stripes: Myosin binding protein-C interactions with actin

Sabine J. Van Dijk, Kristina L. Bezold, Samantha P. Harris

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Myosin binding protein-C (MyBP-C) was first discovered as an impurity during the purification of myosin from skeletal muscle. However, soon after its discovery, MyBP-C was also shown to bind actin. While the unique functional implications for a protein that could cross-link thick and thin filaments together were immediately recognized, most early research nonetheless focused on interactions of MyBP-C with the thick filament. This was in part because interactions of MyBP-C with the thick filament could adequately explain most (but not all) effects of MyBP-C on actomyosin interactions and in part because the specificity of actin binding was uncertain. However, numerous recent studies have now established that MyBP-C can indeed bind to actin through multiple binding sites, some of which are highly specific. Many of these interactions involve critical regulatory domains of MyBP-C that are also reported to interact with myosin. Here we review current evidence supporting MyBP-C interactions with actin and discuss these findings in terms of their ability to account for the functional effects of MyBP-C. We conclude that the influence of MyBP-C on muscle contraction can be explained equally well by interactions with actin as by interactions with myosin. However, because data showing that MyBP-C binds to either myosin or actin has come almost exclusively from in vitro biochemical studies, the challenge for future studies is to define which binding partner(s) MyBP-C interacts with in vivo.

Original languageEnglish (US)
Pages (from-to)445-450
Number of pages6
JournalPflugers Archiv European Journal of Physiology
Volume466
Issue number3
DOIs
StatePublished - Mar 2014
Externally publishedYes

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Keywords

  • Actin
  • Muscle
  • Myosin
  • Myosin binding protein-C
  • Sarcomere

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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