Early spatial and temporal events of human T-lymphotropic virus type 1 spread following blood-borne transmission in a rabbit model of infection

Rashade A H Haynes, Bevin Zimmerman, Laurie Millward, Evan Ware, Christopher Premanandan, Lianbo Yu, Andrew J. Phipps, Michael Dale Lairmore

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Human T-lymphotropic virus type 1 (HTLV-1) infection causes adult T-cell leukemia/lymphoma (ATL) and is associated with a variety of lymphocyte-mediated disorders. HTLV-1 transmission occurs by transmission of infected cells via breast-feeding by infected mothers, sexual intercourse, and contaminated blood products. The route of exposure and early virus replication events are believed to be key determinants of virus-associated spread, antiviral immune responses, and ultimately disease outcomes. The lack of knowledge of early events of HTLV-1 spread following blood-borne transmission of the virus in vivo hinders a more complete understanding of the immunopathogenesis of HTLV-1 infections. Herein, we have used an established animal model of HTLV-1 infection to study early spatial and temporal events of the viral infection. Twelve-week-old rabbits were injected intravenously with cell-associated HTLV-1 (ACH-transformed R49). Blood and tissues were collected at defined intervals throughout the study to test the early spread of the infection. Antibody and hematologic responses were monitored throughout the infection. HTLV-1 intracellular Tax and soluble p19 matrix were tested from ex vivo cultured lymphocytes. Proviral copy numbers were measured by real-time PCR from blood and tissue mononuclear leukocytes. Our data indicate that intravenous infection with cell-associated HTLV-1 targets lymphocytes located in both primary lymphoid and gut-associated lymphoid compartments. A transient lymphocytosis that correlated with peak virus detection parameters was observed by 1 week postinfection before returning to baseline levels. Our data support emerging evidence that HTLV-1 promotes lymphocyte proliferation preceding early viral spread in lymphoid compartments to establish and maintain persistent infection.

Original languageEnglish (US)
Pages (from-to)5124-5130
Number of pages7
JournalJournal of Virology
Volume84
Issue number10
DOIs
StatePublished - May 2010
Externally publishedYes

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Human T-lymphotropic virus 1
rabbits
Rabbits
viruses
blood
Infection
infection
Virus Diseases
Lymphocytes
Viruses
lymphocytes
Mononuclear Leukocytes
Lymphocytosis
Adult T Cell Leukemia Lymphoma
Coitus
exposure pathways
Virus Replication
Breast Feeding
virus transmission
breast feeding

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Early spatial and temporal events of human T-lymphotropic virus type 1 spread following blood-borne transmission in a rabbit model of infection. / Haynes, Rashade A H; Zimmerman, Bevin; Millward, Laurie; Ware, Evan; Premanandan, Christopher; Yu, Lianbo; Phipps, Andrew J.; Lairmore, Michael Dale.

In: Journal of Virology, Vol. 84, No. 10, 05.2010, p. 5124-5130.

Research output: Contribution to journalArticle

Haynes, Rashade A H ; Zimmerman, Bevin ; Millward, Laurie ; Ware, Evan ; Premanandan, Christopher ; Yu, Lianbo ; Phipps, Andrew J. ; Lairmore, Michael Dale. / Early spatial and temporal events of human T-lymphotropic virus type 1 spread following blood-borne transmission in a rabbit model of infection. In: Journal of Virology. 2010 ; Vol. 84, No. 10. pp. 5124-5130.
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