Early redox activities modulate Xenopus tail regeneration

Fernando Ferreira, Vijay Krishna Raghunathan, Guillaume Luxardi, Kan Zhu, Min Zhao

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Redox state sustained by reactive oxygen species (ROS) is crucial for regeneration; however, the interplay between oxygen (O2), ROS and hypoxia-inducible factors (HIF) remains elusive. Here we observe, using an optic-based probe (optrode), an elevated and steady O2 influx immediately upon amputation. The spatiotemporal O2 influx profile correlates with the regeneration of Xenopus laevis tadpole tails. Inhibition of ROS production but not ROS scavenging decreases O2 influx. Inhibition of HIF-1α impairs regeneration and stabilization of HIF-1α induces regeneration in the refractory period. In the regeneration bud, hypoxia correlates with O2 influx, ROS production, and HIF-1α stabilization that modulate regeneration. Further analyses reveal that heat shock protein 90 is a putative downstream target of HIF-1α while electric current reversal is a de facto downstream target of HIF-1α. Collectively, the results show a mechanism for regeneration via the orchestration of O2 influx, ROS production, and HIF-1α stabilization.

Original languageEnglish (US)
Article number4296
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Hypoxia-Inducible Factor 1
hypoxia
Xenopus
regeneration
Oxidation-Reduction
Tail
Regeneration
Reactive Oxygen Species
oxygen
Stabilization
stabilization
HSP90 Heat-Shock Proteins
refractory period
Scavenging
Electric currents
Refractory materials
Xenopus laevis
Amputation
Optics
scavenging

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Early redox activities modulate Xenopus tail regeneration. / Ferreira, Fernando; Raghunathan, Vijay Krishna; Luxardi, Guillaume; Zhu, Kan; Zhao, Min.

In: Nature Communications, Vol. 9, No. 1, 4296, 01.12.2018.

Research output: Contribution to journalArticle

Ferreira, Fernando ; Raghunathan, Vijay Krishna ; Luxardi, Guillaume ; Zhu, Kan ; Zhao, Min. / Early redox activities modulate Xenopus tail regeneration. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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