Early-Onset Progressive Retinal Atrophy Associated with an IQCB1 Variant in African Black-Footed Cats (Felis nigripes)

Annie Oh, Jacqueline W. Pearce, Barbara Gandolfi, Erica K. Creighton, William K. Suedmeyer, Michael Selig, Ann P. Bosiack, Leilani J. Castaner, Rebecca E H Whiting, Ellen B. Belknap, Leslie A Lyons, Danielle Aderdein, Paulo C. Alves, Gregory S. Barsh, Holly C. Beale, Adam R. Boyko, Marta G. Castelhano, Patricia Chan, N. Matthew Ellinwood, Dorian J. GarrickChristopher R. Helps, Christopher B. Kaelin, Tosso Leeb, Hannes Lohi, Maria Longeri, Richard Malik, Michael J. Montague, John S. Munday, William J. Murphy, Niels C Pedersen, Max F. Rothschild, William F. Swanson, Karen A. Terio, Rory J. Todhunter, Wesley C. Warren

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

African black-footed cats (Felis nigripes) are endangered wild felids. One Male and full-sibling feMale African black-footed cat developed vision deficits and mydriasis as early as 3 months of age. The diagnosis of early-onset progressive retinal atrophy (PRA) was supported by reduced direct and consensual pupillary light reflexes, phenotypic presence of retinal degeneration, and a non-recordable electroretinogram with negligible amplitudes in both eyes. Whole genome sequencing, conducted on two unaffected parents and one affected offspring was compared to a variant database from 51 domestic cats and a Pallas cat, revealed 50 candidate variants that segregated concordantly with the PRA phenotype. Testing in additional affected cats confirmed that cats homozygous for a 2 base pair (bp) deletion within IQ calmodulin-binding motif-containing protein-1 (IQCB1), the gene that encodes for nephrocystin-5 (NPHP5), had vision loss. The variant segregated concordantly in other related individuals within the pedigree supporting the identification of a recessively inherited early-onset feline PRA. Analysis of the black-footed cat studbook suggests additional captive cats are at risk. Genetic testing for IQCB1 and avoidance of matings between carriers should be added to the species survival plan for captive management.

Original languageEnglish (US)
Article number43918
JournalScientific Reports
Volume7
DOIs
StatePublished - Mar 21 2017

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Felis
Atrophy
Cats
Pupillary Reflex
Mydriasis
Retinal Degeneration
Amino Acid Motifs
Felidae
Genetic Testing
Calmodulin
Pedigree
Base Pairing
Genome
Databases
Phenotype
Light

ASJC Scopus subject areas

  • General

Cite this

Oh, A., Pearce, J. W., Gandolfi, B., Creighton, E. K., Suedmeyer, W. K., Selig, M., ... Warren, W. C. (2017). Early-Onset Progressive Retinal Atrophy Associated with an IQCB1 Variant in African Black-Footed Cats (Felis nigripes). Scientific Reports, 7, [43918]. https://doi.org/10.1038/srep43918

Early-Onset Progressive Retinal Atrophy Associated with an IQCB1 Variant in African Black-Footed Cats (Felis nigripes). / Oh, Annie; Pearce, Jacqueline W.; Gandolfi, Barbara; Creighton, Erica K.; Suedmeyer, William K.; Selig, Michael; Bosiack, Ann P.; Castaner, Leilani J.; Whiting, Rebecca E H; Belknap, Ellen B.; Lyons, Leslie A; Aderdein, Danielle; Alves, Paulo C.; Barsh, Gregory S.; Beale, Holly C.; Boyko, Adam R.; Castelhano, Marta G.; Chan, Patricia; Ellinwood, N. Matthew; Garrick, Dorian J.; Helps, Christopher R.; Kaelin, Christopher B.; Leeb, Tosso; Lohi, Hannes; Longeri, Maria; Malik, Richard; Montague, Michael J.; Munday, John S.; Murphy, William J.; Pedersen, Niels C; Rothschild, Max F.; Swanson, William F.; Terio, Karen A.; Todhunter, Rory J.; Warren, Wesley C.

In: Scientific Reports, Vol. 7, 43918, 21.03.2017.

Research output: Contribution to journalArticle

Oh, A, Pearce, JW, Gandolfi, B, Creighton, EK, Suedmeyer, WK, Selig, M, Bosiack, AP, Castaner, LJ, Whiting, REH, Belknap, EB, Lyons, LA, Aderdein, D, Alves, PC, Barsh, GS, Beale, HC, Boyko, AR, Castelhano, MG, Chan, P, Ellinwood, NM, Garrick, DJ, Helps, CR, Kaelin, CB, Leeb, T, Lohi, H, Longeri, M, Malik, R, Montague, MJ, Munday, JS, Murphy, WJ, Pedersen, NC, Rothschild, MF, Swanson, WF, Terio, KA, Todhunter, RJ & Warren, WC 2017, 'Early-Onset Progressive Retinal Atrophy Associated with an IQCB1 Variant in African Black-Footed Cats (Felis nigripes)', Scientific Reports, vol. 7, 43918. https://doi.org/10.1038/srep43918
Oh, Annie ; Pearce, Jacqueline W. ; Gandolfi, Barbara ; Creighton, Erica K. ; Suedmeyer, William K. ; Selig, Michael ; Bosiack, Ann P. ; Castaner, Leilani J. ; Whiting, Rebecca E H ; Belknap, Ellen B. ; Lyons, Leslie A ; Aderdein, Danielle ; Alves, Paulo C. ; Barsh, Gregory S. ; Beale, Holly C. ; Boyko, Adam R. ; Castelhano, Marta G. ; Chan, Patricia ; Ellinwood, N. Matthew ; Garrick, Dorian J. ; Helps, Christopher R. ; Kaelin, Christopher B. ; Leeb, Tosso ; Lohi, Hannes ; Longeri, Maria ; Malik, Richard ; Montague, Michael J. ; Munday, John S. ; Murphy, William J. ; Pedersen, Niels C ; Rothschild, Max F. ; Swanson, William F. ; Terio, Karen A. ; Todhunter, Rory J. ; Warren, Wesley C. / Early-Onset Progressive Retinal Atrophy Associated with an IQCB1 Variant in African Black-Footed Cats (Felis nigripes). In: Scientific Reports. 2017 ; Vol. 7.
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abstract = "African black-footed cats (Felis nigripes) are endangered wild felids. One Male and full-sibling feMale African black-footed cat developed vision deficits and mydriasis as early as 3 months of age. The diagnosis of early-onset progressive retinal atrophy (PRA) was supported by reduced direct and consensual pupillary light reflexes, phenotypic presence of retinal degeneration, and a non-recordable electroretinogram with negligible amplitudes in both eyes. Whole genome sequencing, conducted on two unaffected parents and one affected offspring was compared to a variant database from 51 domestic cats and a Pallas cat, revealed 50 candidate variants that segregated concordantly with the PRA phenotype. Testing in additional affected cats confirmed that cats homozygous for a 2 base pair (bp) deletion within IQ calmodulin-binding motif-containing protein-1 (IQCB1), the gene that encodes for nephrocystin-5 (NPHP5), had vision loss. The variant segregated concordantly in other related individuals within the pedigree supporting the identification of a recessively inherited early-onset feline PRA. Analysis of the black-footed cat studbook suggests additional captive cats are at risk. Genetic testing for IQCB1 and avoidance of matings between carriers should be added to the species survival plan for captive management.",
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T1 - Early-Onset Progressive Retinal Atrophy Associated with an IQCB1 Variant in African Black-Footed Cats (Felis nigripes)

AU - Oh, Annie

AU - Pearce, Jacqueline W.

AU - Gandolfi, Barbara

AU - Creighton, Erica K.

AU - Suedmeyer, William K.

AU - Selig, Michael

AU - Bosiack, Ann P.

AU - Castaner, Leilani J.

AU - Whiting, Rebecca E H

AU - Belknap, Ellen B.

AU - Lyons, Leslie A

AU - Aderdein, Danielle

AU - Alves, Paulo C.

AU - Barsh, Gregory S.

AU - Beale, Holly C.

AU - Boyko, Adam R.

AU - Castelhano, Marta G.

AU - Chan, Patricia

AU - Ellinwood, N. Matthew

AU - Garrick, Dorian J.

AU - Helps, Christopher R.

AU - Kaelin, Christopher B.

AU - Leeb, Tosso

AU - Lohi, Hannes

AU - Longeri, Maria

AU - Malik, Richard

AU - Montague, Michael J.

AU - Munday, John S.

AU - Murphy, William J.

AU - Pedersen, Niels C

AU - Rothschild, Max F.

AU - Swanson, William F.

AU - Terio, Karen A.

AU - Todhunter, Rory J.

AU - Warren, Wesley C.

PY - 2017/3/21

Y1 - 2017/3/21

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