Early life allergen and air pollutant exposures alter longitudinal blood immune profiles in infant rhesus monkeys

Candace M. Crowley, Justin H. Fontaine, Joan E. Gerriets, Edward S Schelegle, Dallas M. Hyde, Lisa Miller

Research output: Contribution to journalArticle

Abstract

Early life is a critical period for the progressive establishment of immunity in response to environmental stimuli; the impact of airborne challenges on this process is not well defined. In a longitudinal fashion, we determined the effect of episodic house dust mite (HDM) aerosol and ozone inhalation, both separately and combined, on peripheral blood immune cell phenotypes and cytokine expression from 4 to 25 weeks of age in an infant rhesus monkey model of childhood development. Immune profiles in peripheral blood were compared with lung lavage at 25 weeks of age. Independent of exposure, peripheral blood cell counts fluctuated with chronologic age of animals, while IFNγ and IL-4 mRNA levels increased over time in a linear fashion. At 12 weeks of age, total WBC, lymphocyte numbers, FoxP3 mRNA and IL-12 mRNA were dramatically reduced relative to earlier time points, but increased to a steady state with age. Exposure effects were observed for monocyte numbers, as well as CCR3, FoxP3, and IL-12 mRNA levels in peripheral blood. Significant differences in cell surface marker and cytokine expression were detected following in vitro HDM or PMA/ionomycin stimulation of PBMC isolated from animals exposed to either HDM or ozone. Lavage revealed a mixed immune phenotype of FoxP3, IFNγ and eosinophilia in association with combined HDM plus ozone exposure, which was not observed in blood. Collectively, our findings show that airborne challenges during postnatal development elicit measureable cell and cytokine changes in peripheral blood over time, but exposure-induced immune profiles are not mirrored in the lung.

Original languageEnglish (US)
Pages (from-to)60-69
Number of pages10
JournalToxicology and Applied Pharmacology
Volume328
DOIs
StatePublished - Aug 1 2017

Fingerprint

Pyroglyphidae
Air Pollutants
Macaca mulatta
Allergens
Ozone
Blood
Messenger RNA
Dust
Interleukin-12
Cytokines
Phenotype
Ionomycin
Blood Cell Count
Therapeutic Irrigation
Lymphocyte Count
Eosinophilia
Animals
Bronchoalveolar Lavage
Aerosols
Interleukin-4

Keywords

  • Cytokine
  • House dust mite
  • Infant
  • Ozone
  • Peripheral blood

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

Early life allergen and air pollutant exposures alter longitudinal blood immune profiles in infant rhesus monkeys. / Crowley, Candace M.; Fontaine, Justin H.; Gerriets, Joan E.; Schelegle, Edward S; Hyde, Dallas M.; Miller, Lisa.

In: Toxicology and Applied Pharmacology, Vol. 328, 01.08.2017, p. 60-69.

Research output: Contribution to journalArticle

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