Abstract
Increasing evidence suggests that intraneuronal amyloid-β (Aβ) accumulation may be an early event in Alzheimer's disease (AD) pathogenesis. However direct in vivo evidence regarding initial Aβ seeding is missing. Using an APP transgenic mouse model, our sensitive immunocytochemical procedures revealed a novel intraneuronal Aβ deposition in the somas of hippocampal CAI/subiculum neurons far in advance of the occurrence of extracellular Aβ plaques. These deposits increased exponentially with age and were elevated ∼4-fold (p < 0.001) by high fat/high cholesterol diet. Aβ40 and Aβ42 were the major constituents of these deposits and were co-localized with lysosomal markers. Our results are consistent with the notion that the earliest Aβ deposition occurs intraneuronally, prior to extracellular amyloid plaque formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 123-129 |
| Number of pages | 7 |
| Journal | NeuroReport |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 20 2003 |
| Externally published | Yes |
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Keywords
- Aβ
- Alzheimer's disease
- Cholesterol
- Hippocampus
- Intraneuronal
- Lysosome
- Mice
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Early intraneuronal Aβ deposition in the hippocampus of APP transgenic mice. / Shie, Feng Shiun; LeBoeur, Renée C.; Jin, Lee-Way.
In: NeuroReport, Vol. 14, No. 1, 20.01.2003, p. 123-129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Early intraneuronal Aβ deposition in the hippocampus of APP transgenic mice
AU - Shie, Feng Shiun
AU - LeBoeur, Renée C.
AU - Jin, Lee-Way
PY - 2003/1/20
Y1 - 2003/1/20
N2 - Increasing evidence suggests that intraneuronal amyloid-β (Aβ) accumulation may be an early event in Alzheimer's disease (AD) pathogenesis. However direct in vivo evidence regarding initial Aβ seeding is missing. Using an APP transgenic mouse model, our sensitive immunocytochemical procedures revealed a novel intraneuronal Aβ deposition in the somas of hippocampal CAI/subiculum neurons far in advance of the occurrence of extracellular Aβ plaques. These deposits increased exponentially with age and were elevated ∼4-fold (p < 0.001) by high fat/high cholesterol diet. Aβ40 and Aβ42 were the major constituents of these deposits and were co-localized with lysosomal markers. Our results are consistent with the notion that the earliest Aβ deposition occurs intraneuronally, prior to extracellular amyloid plaque formation.
AB - Increasing evidence suggests that intraneuronal amyloid-β (Aβ) accumulation may be an early event in Alzheimer's disease (AD) pathogenesis. However direct in vivo evidence regarding initial Aβ seeding is missing. Using an APP transgenic mouse model, our sensitive immunocytochemical procedures revealed a novel intraneuronal Aβ deposition in the somas of hippocampal CAI/subiculum neurons far in advance of the occurrence of extracellular Aβ plaques. These deposits increased exponentially with age and were elevated ∼4-fold (p < 0.001) by high fat/high cholesterol diet. Aβ40 and Aβ42 were the major constituents of these deposits and were co-localized with lysosomal markers. Our results are consistent with the notion that the earliest Aβ deposition occurs intraneuronally, prior to extracellular amyloid plaque formation.
KW - Aβ
KW - Alzheimer's disease
KW - Cholesterol
KW - Hippocampus
KW - Intraneuronal
KW - Lysosome
KW - Mice
UR - http://www.scopus.com/inward/record.url?scp=0037454477&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037454477&partnerID=8YFLogxK
U2 - 10.1097/00001756-200301200-00023
DO - 10.1097/00001756-200301200-00023
M3 - Article
VL - 14
SP - 123
EP - 129
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 1
ER -