Early events in naphthalene-induced acute Clara cell toxicity comparison of membrane permeability and ultrastructure

Laura S. Van Winkle, Zachary A. Johnson, Susan J. Nishio, Collette D. Brown, Charles Plopper

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Naphthalene causes severe dose-and site-selective injury to mouse nonciliated bronchiolar (Clara) epithelial cells. Toxicity is characterized by exfoliation of injured Clara cells into the airway lumen 24 h after exposure. The purpose of this study was to define the temporal pattern of intracellular changes immediately following naphthalene treatment, with the goal of identifying critical early events involved in cytotoxicity. Mice were injected with naphthalene or carrier and were killed 1, 2, 3, and 6 h after treatment (PT). Loss of membrane integrity was assessed by ethidium homodimer-1 permeability and confocal microscopy. Cell morphology and ultrastructure were evaluated using high-resolution light and electron microscopy. Permeable cells were found only in terminal bronchioles and increased in abundance with time PT. At 2 and 3 h PT, when most Clara cells had early signs of injury, few permeable cells were detected. Many Clara cells had apical membrane blebs that contained abundant, swollen, smooth endoplasmic reticulum (SER) and few other organelles. By 6 h PT many Clara cells were membrane-permeable. However, many permeable Clara cells lacked apical blebs and SER was less abundant in these cells. Cytoplasmic blebbing may be a mechanism to protect the cell by isolating and removing damaged SER. We conclude that the early stages of injury include SER swelling and bleb formation which precede increases in cell membrane permeability after acute naphthalene injury to bronchiolar Clara cells in vivo.

Original languageEnglish (US)
Pages (from-to)44-53
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume21
Issue number1
StatePublished - 1999

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Toxicity
Permeability
Membranes
Cell membranes
Smooth Endoplasmic Reticulum
Blister
Confocal microscopy
Cytotoxicity
Electron microscopy
Optical microscopy
Swelling
Wounds and Injuries
naphthalene
Cell Membrane Permeability
Bronchioles
Confocal Microscopy
Organelles
Electron Microscopy
Epithelial Cells
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Early events in naphthalene-induced acute Clara cell toxicity comparison of membrane permeability and ultrastructure. / Van Winkle, Laura S.; Johnson, Zachary A.; Nishio, Susan J.; Brown, Collette D.; Plopper, Charles.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 21, No. 1, 1999, p. 44-53.

Research output: Contribution to journalArticle

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