Early bioelectric activities mediate redox-modulated regeneration

Fernando Ferreira, Guillaume Luxardi, Brian Reid, Min Zhao

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) and electric currents modulate regeneration; however, the interplay between biochemical and biophysical signals during regeneration remains poorly understood. We investigate the interactions between redox and bioelectric activities during tail regeneration in Xenopus laevis tadpoles. We show that inhibition of NADPH oxidase-mediated production of ROS, or scavenging or blocking their diffusion into cells, impairs regeneration and consistently regulates the dynamics of membrane potential, transepithelial potential (TEP) and electric current densities (JI) during regeneration. Depletion of ROS mimics the altered TEP and JI observed in the non-regenerative refractory period. Short-term application of hydrogen peroxide (H2O2) rescues (from depleted ROS) and induces (from the refractory period) regeneration, TEP increase and JI reversal. H2O2 is therefore necessary for and sufficient to induce regeneration and to regulate TEP and JI. Epistasis assays show that voltage-gated Na+ channels act downstream of H2O2 to modulate regeneration. Altogether, these results suggest a novel mechanism for regeneration via redoxbioelectric orchestration.

Original languageEnglish (US)
Pages (from-to)4582-4594
Number of pages13
JournalDevelopment (Cambridge)
Volume143
Issue number24
DOIs
StatePublished - Dec 15 2016

Fingerprint

Oxidation-Reduction
Regeneration
Reactive Oxygen Species
NADPH Oxidase
Xenopus laevis
Membrane Potentials
Hydrogen Peroxide
Larva
Tail

Keywords

  • Electric current density
  • Membrane potential
  • NADPH oxidases
  • Reactive oxygen species
  • Regeneration
  • Transepithelial potential
  • Voltage-gated Na channels
  • Xenopus laevis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Early bioelectric activities mediate redox-modulated regeneration. / Ferreira, Fernando; Luxardi, Guillaume; Reid, Brian; Zhao, Min.

In: Development (Cambridge), Vol. 143, No. 24, 15.12.2016, p. 4582-4594.

Research output: Contribution to journalArticle

Ferreira, Fernando ; Luxardi, Guillaume ; Reid, Brian ; Zhao, Min. / Early bioelectric activities mediate redox-modulated regeneration. In: Development (Cambridge). 2016 ; Vol. 143, No. 24. pp. 4582-4594.
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