E-tubulin is required for centriole duplication and microtubule organization

Paul Chang, Thomas H. Giddings, Mark Winey, Tim Stearns

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified E-tubulin as a new member of the tubulin super-family that localizes asymmetrically to the two centrosomes after duplication. We show that recruitment of E-tubulin to the new centrosome can only occur after exit from S phase and that E-tubulin is associated with the sub-distal appendages of mature centrioles. Xenopus laevis E-tubulin was cloned and shown to be similar to human E-tubulin in both sequence and localization. Depletion of E-tubulin from Xenopus egg extracts blocks centriole duplication in S phase and formation of organized centrosome-independent microtubule asters in M phase. We conclude that E-tubulin is a component of the sub-distal appendages of the centriole, explaining its asymmetric localization to old and new centrosomes, and that E-tubulin is required for centriole duplication and organization of the pericentriolar material.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalNature Cell Biology
Volume5
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

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Centrioles
Tubulin
Microtubules
Centrosome
S Phase
Spindle Apparatus
Xenopus laevis
Xenopus
Cell Division
Ovum
Cell Cycle

ASJC Scopus subject areas

  • Cell Biology

Cite this

E-tubulin is required for centriole duplication and microtubule organization. / Chang, Paul; Giddings, Thomas H.; Winey, Mark; Stearns, Tim.

In: Nature Cell Biology, Vol. 5, No. 1, 01.01.2003, p. 71-76.

Research output: Contribution to journalArticle

Chang, Paul ; Giddings, Thomas H. ; Winey, Mark ; Stearns, Tim. / E-tubulin is required for centriole duplication and microtubule organization. In: Nature Cell Biology. 2003 ; Vol. 5, No. 1. pp. 71-76.
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