Abstract
Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified E-tubulin as a new member of the tubulin super-family that localizes asymmetrically to the two centrosomes after duplication. We show that recruitment of E-tubulin to the new centrosome can only occur after exit from S phase and that E-tubulin is associated with the sub-distal appendages of mature centrioles. Xenopus laevis E-tubulin was cloned and shown to be similar to human E-tubulin in both sequence and localization. Depletion of E-tubulin from Xenopus egg extracts blocks centriole duplication in S phase and formation of organized centrosome-independent microtubule asters in M phase. We conclude that E-tubulin is a component of the sub-distal appendages of the centriole, explaining its asymmetric localization to old and new centrosomes, and that E-tubulin is required for centriole duplication and organization of the pericentriolar material.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 71-76 |
| Number of pages | 6 |
| Journal | Nature Cell Biology |
| Volume | 5 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2003 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
Cite this
E-tubulin is required for centriole duplication and microtubule organization. / Chang, Paul; Giddings, Thomas H.; Winey, Mark; Stearns, Tim.
In: Nature Cell Biology, Vol. 5, No. 1, 01.01.2003, p. 71-76.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - E-tubulin is required for centriole duplication and microtubule organization
AU - Chang, Paul
AU - Giddings, Thomas H.
AU - Winey, Mark
AU - Stearns, Tim
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified E-tubulin as a new member of the tubulin super-family that localizes asymmetrically to the two centrosomes after duplication. We show that recruitment of E-tubulin to the new centrosome can only occur after exit from S phase and that E-tubulin is associated with the sub-distal appendages of mature centrioles. Xenopus laevis E-tubulin was cloned and shown to be similar to human E-tubulin in both sequence and localization. Depletion of E-tubulin from Xenopus egg extracts blocks centriole duplication in S phase and formation of organized centrosome-independent microtubule asters in M phase. We conclude that E-tubulin is a component of the sub-distal appendages of the centriole, explaining its asymmetric localization to old and new centrosomes, and that E-tubulin is required for centriole duplication and organization of the pericentriolar material.
AB - Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified E-tubulin as a new member of the tubulin super-family that localizes asymmetrically to the two centrosomes after duplication. We show that recruitment of E-tubulin to the new centrosome can only occur after exit from S phase and that E-tubulin is associated with the sub-distal appendages of mature centrioles. Xenopus laevis E-tubulin was cloned and shown to be similar to human E-tubulin in both sequence and localization. Depletion of E-tubulin from Xenopus egg extracts blocks centriole duplication in S phase and formation of organized centrosome-independent microtubule asters in M phase. We conclude that E-tubulin is a component of the sub-distal appendages of the centriole, explaining its asymmetric localization to old and new centrosomes, and that E-tubulin is required for centriole duplication and organization of the pericentriolar material.
UR - http://www.scopus.com/inward/record.url?scp=0037228254&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037228254&partnerID=8YFLogxK
U2 - 10.1038/ncb900
DO - 10.1038/ncb900
M3 - Article
C2 - 12510196
AN - SCOPUS:0037228254
VL - 5
SP - 71
EP - 76
JO - Nature Cell Biology
JF - Nature Cell Biology
SN - 1465-7392
IS - 1
ER -