Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified E-tubulin as a new member of the tubulin super-family that localizes asymmetrically to the two centrosomes after duplication. We show that recruitment of E-tubulin to the new centrosome can only occur after exit from S phase and that E-tubulin is associated with the sub-distal appendages of mature centrioles. Xenopus laevis E-tubulin was cloned and shown to be similar to human E-tubulin in both sequence and localization. Depletion of E-tubulin from Xenopus egg extracts blocks centriole duplication in S phase and formation of organized centrosome-independent microtubule asters in M phase. We conclude that E-tubulin is a component of the sub-distal appendages of the centriole, explaining its asymmetric localization to old and new centrosomes, and that E-tubulin is required for centriole duplication and organization of the pericentriolar material.
ASJC Scopus subject areas
- Cell Biology