Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters

Carrie T. Drake, Gregory W. Terman, Michele L. Simmons, Teresa A. Milner, Dennis D. Kunkel, Philip A Schwartzkroin, Charles Chavkin

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

The granule cell population response to perforant path stimulation decreased significantly within seconds following release of endogenous dynorphin from dentate granule cells. The depression was blocked by the opioid receptor antagonists naloxone and norbinaltorphimine, suggesting that the effect was mediated by dynorphin activation of κ(i) type opioid receptors. Pharmacological application of dynorphin B in the molecular layer was effective at reducing excitatory synaptic transmission from the perforant path, but application in the hilus had no significant effect. These results suggest that endogenous dynorphin peptides may be released from a local source within the dentate molecular layer. By light microscopy, dynorphin- like immunoreactivity (dynorphin-LI) was primarily found in granule cell axons in the hilus and stratum lucidum with only a few scattered fibers evident in the molecular layer. At the extreme ventral pole of the hippocampus, a diffuse band of varicose processes was also seen in the molecular layer, but this band was not present in more dorsal sections similar to those used for the electrophysiological studies. Electron microscopic analysis of the molecular layer midway along the septotemporal axis revealed that dynorphin-LI was present in dense-core vesicles in both spiny dendrites and unmyelinated axons with the majority (74%) of the dynorphin-LI-containing dense-core vesicles found in dendrites. Neuronal processes containing dynorphin-LI were observed throughout the molecular layer. The results suggest that dynorphin release from granule cell processes in the molecular layer regulates excitatory inputs entering the hippocampus from cerebral cortex, thus potentially counteracting such excitation-induced phenomena as epileptogenesis or long-term potentiation.

Original languageEnglish (US)
Pages (from-to)3736-3750
Number of pages15
JournalJournal of Neuroscience
Volume14
Issue number6
StatePublished - Jun 1994
Externally publishedYes

Fingerprint

Dynorphins
Opioid Analgesics
Neurotransmitter Agents
Perforant Pathway
Secretory Vesicles
Dendrites
Axons
Hippocampus
Narcotic Antagonists
Long-Term Potentiation
Opioid Receptors
Naloxone
Synaptic Transmission
Cerebral Cortex
Microscopy
Pharmacology
Electrons
Light
Peptides

Keywords

  • dendritic transmitter release
  • electron microscopy
  • endogenous opioids
  • hippocampus
  • light microscopy
  • peptide release

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Drake, C. T., Terman, G. W., Simmons, M. L., Milner, T. A., Kunkel, D. D., Schwartzkroin, P. A., & Chavkin, C. (1994). Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters. Journal of Neuroscience, 14(6), 3736-3750.

Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters. / Drake, Carrie T.; Terman, Gregory W.; Simmons, Michele L.; Milner, Teresa A.; Kunkel, Dennis D.; Schwartzkroin, Philip A; Chavkin, Charles.

In: Journal of Neuroscience, Vol. 14, No. 6, 06.1994, p. 3736-3750.

Research output: Contribution to journalArticle

Drake, CT, Terman, GW, Simmons, ML, Milner, TA, Kunkel, DD, Schwartzkroin, PA & Chavkin, C 1994, 'Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters', Journal of Neuroscience, vol. 14, no. 6, pp. 3736-3750.
Drake, Carrie T. ; Terman, Gregory W. ; Simmons, Michele L. ; Milner, Teresa A. ; Kunkel, Dennis D. ; Schwartzkroin, Philip A ; Chavkin, Charles. / Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters. In: Journal of Neuroscience. 1994 ; Vol. 14, No. 6. pp. 3736-3750.
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