Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles

Rebecca S. Brogan, Margaret MacGibeny, Scott Mix, Christopher Thompson, Muraly Puttabyatappa, Catherine A. VandeVoort, Charles L. Chaffin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glucose is important to the maturation of the oocyte and development of the embryo, while hyperglycemia results in profound reproductive and developmental consequences. However, the normal physiology of glucose in the ovary remains poorly understood. The goal of this study was to determine intra-follicular glucose dynamics during the periovulatory interval in non-human primates undergoing controlled ovarian stimulation protocols. Follicular fluid and mural granulosa cells were isolated before or up to 24. h after an ovulatory hCG bolus, and the human granulosa-lutein cell line hGL5 was used. Intra-follicular glucose increased 3. h after hCG, and remained at that level until 12. h when levels decline back to pre-hCG concentrations. Pyruvate and lactate concentrations in the follicle were not strongly altered by hCG. Mural granulosa cell expression of hexokinase 1 and 2, and glucose-6-phosphate dehydrogenase mRNA decreased following hCG, while glycogen phosphorylase (liver form) increased following hCG. Glucose uptake by hGL5 cells was delayed until 24. h following stimulation. In summary, intra-follicular glucose increases following an ovulatory stimulus and mural granulosa cells do not appear able to utilize it, sparing the glucose for the cumulus-oocyte complex.

Original languageEnglish (US)
Pages (from-to)189-195
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume332
Issue number1-2
DOIs
StatePublished - Jan 30 2011

Fingerprint

Luteinization
Ovarian Follicle
Macaca
Glucose
Granulosa Cells
Liver Form Glycogen Phosphorylase
Oocytes
Luteal Cells
Lutein
Follicular Fluid
Hexokinase
Ovulation Induction
Glucosephosphate Dehydrogenase
Physiology
Pyruvic Acid
Hyperglycemia
Primates
Embryonic Development
Ovary
Lactic Acid

Keywords

  • Glucose
  • Glycolysis
  • Granulosa cells
  • Luteinization
  • Macaque

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Brogan, R. S., MacGibeny, M., Mix, S., Thompson, C., Puttabyatappa, M., VandeVoort, C. A., & Chaffin, C. L. (2011). Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles. Molecular and Cellular Endocrinology, 332(1-2), 189-195. https://doi.org/10.1016/j.mce.2010.10.011

Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles. / Brogan, Rebecca S.; MacGibeny, Margaret; Mix, Scott; Thompson, Christopher; Puttabyatappa, Muraly; VandeVoort, Catherine A.; Chaffin, Charles L.

In: Molecular and Cellular Endocrinology, Vol. 332, No. 1-2, 30.01.2011, p. 189-195.

Research output: Contribution to journalArticle

Brogan, RS, MacGibeny, M, Mix, S, Thompson, C, Puttabyatappa, M, VandeVoort, CA & Chaffin, CL 2011, 'Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles', Molecular and Cellular Endocrinology, vol. 332, no. 1-2, pp. 189-195. https://doi.org/10.1016/j.mce.2010.10.011
Brogan RS, MacGibeny M, Mix S, Thompson C, Puttabyatappa M, VandeVoort CA et al. Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles. Molecular and Cellular Endocrinology. 2011 Jan 30;332(1-2):189-195. https://doi.org/10.1016/j.mce.2010.10.011
Brogan, Rebecca S. ; MacGibeny, Margaret ; Mix, Scott ; Thompson, Christopher ; Puttabyatappa, Muraly ; VandeVoort, Catherine A. ; Chaffin, Charles L. / Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles. In: Molecular and Cellular Endocrinology. 2011 ; Vol. 332, No. 1-2. pp. 189-195.
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