Abstract
Glucose is important to the maturation of the oocyte and development of the embryo, while hyperglycemia results in profound reproductive and developmental consequences. However, the normal physiology of glucose in the ovary remains poorly understood. The goal of this study was to determine intra-follicular glucose dynamics during the periovulatory interval in non-human primates undergoing controlled ovarian stimulation protocols. Follicular fluid and mural granulosa cells were isolated before or up to 24. h after an ovulatory hCG bolus, and the human granulosa-lutein cell line hGL5 was used. Intra-follicular glucose increased 3. h after hCG, and remained at that level until 12. h when levels decline back to pre-hCG concentrations. Pyruvate and lactate concentrations in the follicle were not strongly altered by hCG. Mural granulosa cell expression of hexokinase 1 and 2, and glucose-6-phosphate dehydrogenase mRNA decreased following hCG, while glycogen phosphorylase (liver form) increased following hCG. Glucose uptake by hGL5 cells was delayed until 24. h following stimulation. In summary, intra-follicular glucose increases following an ovulatory stimulus and mural granulosa cells do not appear able to utilize it, sparing the glucose for the cumulus-oocyte complex.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 189-195 |
| Number of pages | 7 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 332 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jan 30 2011 |
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Keywords
- Glucose
- Glycolysis
- Granulosa cells
- Luteinization
- Macaque
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Biochemistry
Cite this
Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles. / Brogan, Rebecca S.; MacGibeny, Margaret; Mix, Scott; Thompson, Christopher; Puttabyatappa, Muraly; VandeVoort, Catherine A.; Chaffin, Charles L.
In: Molecular and Cellular Endocrinology, Vol. 332, No. 1-2, 30.01.2011, p. 189-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles
AU - Brogan, Rebecca S.
AU - MacGibeny, Margaret
AU - Mix, Scott
AU - Thompson, Christopher
AU - Puttabyatappa, Muraly
AU - VandeVoort, Catherine A.
AU - Chaffin, Charles L.
PY - 2011/1/30
Y1 - 2011/1/30
N2 - Glucose is important to the maturation of the oocyte and development of the embryo, while hyperglycemia results in profound reproductive and developmental consequences. However, the normal physiology of glucose in the ovary remains poorly understood. The goal of this study was to determine intra-follicular glucose dynamics during the periovulatory interval in non-human primates undergoing controlled ovarian stimulation protocols. Follicular fluid and mural granulosa cells were isolated before or up to 24. h after an ovulatory hCG bolus, and the human granulosa-lutein cell line hGL5 was used. Intra-follicular glucose increased 3. h after hCG, and remained at that level until 12. h when levels decline back to pre-hCG concentrations. Pyruvate and lactate concentrations in the follicle were not strongly altered by hCG. Mural granulosa cell expression of hexokinase 1 and 2, and glucose-6-phosphate dehydrogenase mRNA decreased following hCG, while glycogen phosphorylase (liver form) increased following hCG. Glucose uptake by hGL5 cells was delayed until 24. h following stimulation. In summary, intra-follicular glucose increases following an ovulatory stimulus and mural granulosa cells do not appear able to utilize it, sparing the glucose for the cumulus-oocyte complex.
AB - Glucose is important to the maturation of the oocyte and development of the embryo, while hyperglycemia results in profound reproductive and developmental consequences. However, the normal physiology of glucose in the ovary remains poorly understood. The goal of this study was to determine intra-follicular glucose dynamics during the periovulatory interval in non-human primates undergoing controlled ovarian stimulation protocols. Follicular fluid and mural granulosa cells were isolated before or up to 24. h after an ovulatory hCG bolus, and the human granulosa-lutein cell line hGL5 was used. Intra-follicular glucose increased 3. h after hCG, and remained at that level until 12. h when levels decline back to pre-hCG concentrations. Pyruvate and lactate concentrations in the follicle were not strongly altered by hCG. Mural granulosa cell expression of hexokinase 1 and 2, and glucose-6-phosphate dehydrogenase mRNA decreased following hCG, while glycogen phosphorylase (liver form) increased following hCG. Glucose uptake by hGL5 cells was delayed until 24. h following stimulation. In summary, intra-follicular glucose increases following an ovulatory stimulus and mural granulosa cells do not appear able to utilize it, sparing the glucose for the cumulus-oocyte complex.
KW - Glucose
KW - Glycolysis
KW - Granulosa cells
KW - Luteinization
KW - Macaque
UR - http://www.scopus.com/inward/record.url?scp=78650519572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650519572&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2010.10.011
DO - 10.1016/j.mce.2010.10.011
M3 - Article
C2 - 20969917
AN - SCOPUS:78650519572
VL - 332
SP - 189
EP - 195
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -