Dynamics of connective-tissue localization during chronic Borrelia burgdorferi infection

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The etiologic agent of Lyme disease, Borrelia burgdorferi, localizes preferentially in the extracellular matrix during persistence. In chronically infected laboratory mice, there is a direct association between B. burgdorferi and the proteoglycan decorin, which suggests that decorin has a role in defining protective niches for persistent spirochetes. In this study, the tissue colocalization of B. burgdorferi with decorin and the dynamics of borrelial decorin tropism were evaluated during chronic infection. Spirochetes were found to colocalize absolutely with decorin, but not collagen I in chronically infected immunocompetent C3H mice. Passive immunization of infected C3H-scid mice with B. burgdorferi-specific immune serum resulted in the localization of spirochetes in decorin-rich microenvironments, with clearance of spirochetes from decorin-poor microenvironments. In passively immunized C3H-scid mice, tissue spirochete burdens were initially reduced, but increased over time as the B. burgdorferi-specific antibody levels waned. Concurrent repopulation of the previously cleared decorin-poor microenvironments was observed with the rising tissue spirochete burden and declining antibody titer. These findings indicate that the specificity of B. burgdorferi tissue localization during chronic infection is determined by decorin, driven by the borrelia-specific antibody response, and fluctuates with the antibody response.

Original languageEnglish (US)
Pages (from-to)900-910
Number of pages11
JournalLaboratory Investigation
Volume93
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Borrelia Infections
Decorin
Borrelia burgdorferi
Connective Tissue
Spirochaetales
Inbred C3H Mouse
Antibody Formation
Borrelia
Tropism
Passive Immunization
Lyme Disease
Antibodies
Proteoglycans
Infection
Extracellular Matrix
Immune Sera
Collagen

Keywords

  • Borrelia burgdorferi
  • Decorin
  • Decorin-binding proteins
  • Lyme disease
  • Mouse model

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Dynamics of connective-tissue localization during chronic Borrelia burgdorferi infection. / Imai, Denise; Feng, Sunlian; Hodzic, Emir; Barthold, Stephen W.

In: Laboratory Investigation, Vol. 93, No. 8, 08.2013, p. 900-910.

Research output: Contribution to journalArticle

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