Dynamics of antifreeze glycoproteins in the presence of ice

Nelly M. Tsvetkova, Brian L. Phillips, Viswanathan V Krishnan, Robert E. Feeney, William H. Fink, John H. Crowe, Subhash H. Risbud, Fern Tablin, Yin Yen

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38 Scopus citations

Abstract

Antifreeze glycoproteins from the Greenland cod Boreogadus saida were dimethylated at the N-terminus (m*AFGP) and their dynamics and conformational properties were studied in the presence of ice using 13C-NMR and FTIR spectroscopy. 13C-NMR experiments of m*AFGP in D2O, in H2O, and of freeze-dried m*AFGP were performed as a function of temperature. Dynamic parameters (1H T1p and TCH) obtained by varying the contact time revealed notable differences in the motional properties of AFGP between the different states. AFGP/ice dynamics was dominated by fast-scale motions (nanosecond to picosecond time scale), suggesting that the relaxation is markedly affected by the protein hydration. The data suggest that AFGP adopts a similar type of three-dimensional fold both in the presence of ice and in the freeze-dried state. FTIR studies of the amide I band did not show a single prevailing secondary structure in the frozen state. The high number of conformers suggests a high flexibility, and possibly reflects the necessity to expose more ice-binding groups. The data suggest that the effect of hydration on the local mobility of AFGP and the lack of significant change in the backbone conformation in the frozen state may play a role in inhibiting the ice crystal growth.

Original languageEnglish (US)
Pages (from-to)464-473
Number of pages10
JournalBiophysical Journal
Volume82
Issue number1
StatePublished - 2002

ASJC Scopus subject areas

  • Biophysics

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    Tsvetkova, N. M., Phillips, B. L., Krishnan, V. V., Feeney, R. E., Fink, W. H., Crowe, J. H., Risbud, S. H., Tablin, F., & Yen, Y. (2002). Dynamics of antifreeze glycoproteins in the presence of ice. Biophysical Journal, 82(1), 464-473.